scholarly journals Air-Liquid Interface System To Understand Epstein-Barr Virus-Associated Nasopharyngeal Carcinoma

mSphere ◽  
2018 ◽  
Vol 3 (4) ◽  
Author(s):  
Sharon E. Hopcraft ◽  
Cary A. Moody ◽  
Blossom Damania
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Cell Types ◽  
Liquid Interface ◽  
Carcinoma Cells ◽  
Easy Method ◽  
Barr Virus ◽  
Ebv Reactivation ◽  
Epstein Barr ◽  
Air Liquid Interface

ABSTRACT Epstein-Barr virus (EBV) infects epithelial cells and is associated with epithelial malignancies. Although EBV reactivation is induced by epithelial differentiation, the available methods for differentiation are not widely used. In a recent study, Caves et al. (mSphere 3:e00152-18, 2018, https://doi.org/10.1128/mSphere.00152-18) explored the use of a new transwell-based air-liquid interface (ALI) system to differentiate EBV-infected nasopharyngeal carcinoma cells. They found that cells cultured in the ALI system expressed markers of differentiation and supported complete EBV reactivation. This system offers an easy method for differentiation that could be widely adopted. This system could be extended to other epithelial cell types.

scholarly journals A three-dimensional Air-Liquid Interface Culture Model for the Study of Epstein-Barr virus Infection in the Nasopharynx

2020 ◽  
Author(s):  
Phillip Ziegler ◽  
Yulong Bai ◽  
Yarong Tian ◽  
Sanna Abrahamsson ◽  
Anthony Green ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
Three Dimensional ◽  
Cell Types ◽  
Liquid Interface ◽  
Epstein Barr Virus Infection ◽  
Barr Virus ◽  
Ebv Infection ◽  
Transcription Profiles ◽  
Epstein Barr ◽  
Air Liquid Interface

ABSTRACTEpstein-Barr virus (EBV) infection is ubiquitous in humans and is associated with the cancer, nasopharyngeal carcinoma. EBV replicates in the differentiated layers of stratified keratinocytes but whether the other cell types of the airway epithelium are susceptible to EBV is unknown. Here, we demonstrate with primary nasopharyngeal cells grown at the air-liquid interface that the pseudostratified epithelium can be susceptible to EBV infection and we report that susceptible cell types with distinct EBV transcription profiles can be identified by single-cell RNA-sequencing. Although EBV infection in the nasopharynx has evaded detection in asymptomatic carriers, these findings demonstrate that EBV latent and lytic infection can occur in the cells of the nasopharyngeal epithelium.

scholarly journals Epstein-Barr virus-encoded LMP1 regulated Pim1 kinase expression promotes nasopharyngeal carcinoma cells proliferation

2019 ◽  
Vol Volume 12 ◽  
pp. 1137-1146 ◽  
Author(s):  
Ran-ran Ding ◽  
Jian-ling Yuan ◽  
Ya-nan Jia ◽  
Xiao-min Liao ◽  
Si-si Wang ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Carcinoma Cells ◽  
Barr Virus ◽  
Pim1 Kinase ◽  
Epstein Barr ◽  
Kinase Expression

scholarly journals Epstein-Barr Virus Zta-Induced Immunomodulators from Nasopharyngeal Carcinoma Cells Upregulate Interleukin-10 Production from Monocytes

2011 ◽  
Vol 85 (14) ◽  
pp. 7333-7342 ◽  
Author(s):  
C.-H. Lee ◽  
T.-H. Yeh ◽  
H.-C. Lai ◽  
S.-Y. Wu ◽  
I.-J. Su ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Interleukin 10 ◽  
Carcinoma Cells ◽  
Barr Virus ◽  
Epstein Barr

NF-κB inhibitors induce lytic cytotoxicity in Epstein-Barr virus-positive nasopharyngeal carcinoma cells

2008 ◽  
Vol 32 (8) ◽  
pp. 1006-1013 ◽  
Author(s):  
Su-Fang Liu ◽  
Hai Wang ◽  
Xu-Chi Lin ◽  
Hui Xiang ◽  
Xi-Yun Deng ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Carcinoma Cells ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Response of Nasopharyngeal Carcinoma Cells to Epstein-Barr Virus Infection In Vitro

2000 ◽  
Vol 80 (8) ◽  
pp. 1149-1160 ◽  
Author(s):  
Chin-Tarng Lin ◽  
Hsiao-Jung Kao ◽  
Jau-Liang Lin ◽  
Wing-Yee Chan ◽  
Han-Chung Wu ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Virus Infection ◽  
Epstein Barr Virus ◽  
Carcinoma Cells ◽  
Epstein Barr Virus Infection ◽  
Barr Virus ◽  
Epstein Barr ◽  
Barr Virus Infection
Sign in / Sign up

Export Citation Format

Share Document