scholarly journals Effect of transport medium and transportation time on culture of Helicobacter pylori from gastric biopsy specimens.

1993 ◽  
Vol 46 (6) ◽  
pp. 561-563 ◽  
Author(s):  
R A Veenendaal ◽  
A T Lichtendahl-Bernards ◽  
A S Pena ◽  
H P Endtz ◽  
C P van Boven ◽  
...  
1998 ◽  
Vol 36 (10) ◽  
pp. 3048-3050 ◽  
Author(s):  
L. K. Siu ◽  
W. K. Leung ◽  
A. F. B. Cheng ◽  
J. Y. Sung ◽  
T. K. W. Ling ◽  
...  

Since the means of culturing Helicobacter pylori may not be available in some laboratories, prolonging the survival of this organism during transportation is a major concern in terms of improving detection rates. A selective transport medium was evaluated for the preservation of H. pylori from 254 gastric biopsy specimens collected from a rural area in China where culturing is not feasible. Gastric biopsy specimens were inoculated in sterile broth consisting of brain heart infusion (BHI) broth, horse serum, and yeast extract supplemented with vancomycin, amphotericin B, and nalidixic acid (VAN). Of the 254 biopsy specimens, 238 were identified by histology to haveH. pylori infection. Total rates of recovery ofH. pylori from the H. pylori-positive gastric biopsy specimens stored in the BHI-VAN broth ranged from 76 to 46% after storage of specimens for 5 to 9 days. In conclusion, the selective medium is useful for prolonging the survival of H. pylori in gastric biopsy specimens for which immediate culture is not feasible.


2015 ◽  
Vol 83 (2) ◽  
pp. 89-92 ◽  
Author(s):  
Júlia Silveira Vianna ◽  
Ivy Bastos Ramis ◽  
Priscila Cristina Bartolomeu Halicki ◽  
Otávio Leite Gastal ◽  
Renato Azevedo Silva ◽  
...  

2007 ◽  
Vol 10 (22) ◽  
pp. 4156-4159 ◽  
Author(s):  
M.Y. Alikhani ◽  
N. Sadeghifar ◽  
Safar Farajnia . ◽  
M. Hajia ◽  
M.M. Aslani ◽  
...  

2012 ◽  
Vol 43 (3) ◽  
pp. 903-908 ◽  
Author(s):  
Ivy Bastos Ramis ◽  
Ernani Pinho de Moraes ◽  
Márcia Silveira Fernandes ◽  
Raul Mendoza-Sassi ◽  
Obirajara Rodrigues ◽  
...  

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