Helicobacter pylori in gastric biopsy specimens. Comparison of culture, modified Giemsa stain, and immunohistochemistry. A retrospective study

1991 ◽  
Vol 165 (1) ◽  
pp. 69-73 ◽  
Author(s):  
R. J. L. F. Loffeld ◽  
E. Stobberingh ◽  
J. A. Flendrig ◽  
J. W. Arends
Keyword(s):  
Helicobacter Pylori ◽  
Retrospective Study ◽  
Gastric Biopsy ◽  
Giemsa Stain ◽  
Biopsy Specimens

scholarly journals Detection of Helicobacter pylori in gastric biopsy and resection specimens

2005 ◽  
Vol 62 (1) ◽  
pp. 39-43 ◽  
Author(s):  
Tatjana Babic ◽  
Hakija Basic ◽  
Biljana Selimovic-Miljkovic ◽  
Branislava Kocic ◽  
Gordana Tasic
Keyword(s):  
Duodenal Ulcer ◽  
Alkaline Phosphatase ◽  
Helicobacter Pylori ◽  
Gastric Biopsy ◽  
Giemsa Stain ◽  
Biopsy Specimens ◽  
H Pylori ◽  
Antral Biopsy ◽  
Better Than ◽  
Immunohistochemical Identification

Aim. To compare the sensitivity of detecting H. pylori in gastric biopsy and resection specimens using modified Giemsa stain and immunohistochemistry, using a commercially available anti-H. pylori antibody (Dako, Denmark). Methods. Gastric antral biopsy specimens showing chronic gastritis (28 cases) together with tissue blocks from gastrectomy specimens for duodenal ulcer (2 cases) were stained with modified Giemsa and immunoenzymatic alkaline phosphatase - anti-alkaline phosphatase (APAAP) method, and were carefully examined for the presence of H. pylori. Results. Using a modified Giemsa stain, the spiral shaped bacteria of H. pylori stained blue, were attached to the brush border of the gastric foveolar epithelial cells. However, the specificity of modified Giemsa stain depended on the morphological appearance of H. pylori. The specificity of immunostaining permitted detection of low numbers or even single organisms. In all cases bacteria were more prominent and easier to detect in immunostained preparations. H. pylori was identified in 22 (73.3%) of 30 sections stained with modified Giemsa stain, but it could be identified with greater frequency in sections stained with APAAP, in 27 (90%) of 30 sections. Conclusion. Immunohistochemical identification of H. pylori was better than Giemsa stain for detecting that organism.

Detection of Helicobacter pylori CagA EPIYA in gastric biopsy specimens and its relation to gastric diseases

2015 ◽  
Vol 83 (2) ◽  
pp. 89-92 ◽  
Author(s):  
Júlia Silveira Vianna ◽  
Ivy Bastos Ramis ◽  
Priscila Cristina Bartolomeu Halicki ◽  
Otávio Leite Gastal ◽  
Renato Azevedo Silva ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Biopsy ◽  
Gastric Diseases ◽  
Biopsy Specimens

Evaluation of Selective and Nonselective Media for Isolation of Helicobacter pylori from Gastric Biopsy Specimens

2007 ◽  
Vol 10 (22) ◽  
pp. 4156-4159 ◽  
Author(s):  
M.Y. Alikhani ◽  
N. Sadeghifar ◽  
Safar Farajnia . ◽  
M. Hajia ◽  
M.M. Aslani ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Biopsy ◽  
Biopsy Specimens

scholarly journals Evaluation of diagnostic methods for the detection of Helicobacter pylori in gastric biopsy specimens of dyspeptic patients

2012 ◽  
Vol 43 (3) ◽  
pp. 903-908 ◽  
Author(s):  
Ivy Bastos Ramis ◽  
Ernani Pinho de Moraes ◽  
Márcia Silveira Fernandes ◽  
Raul Mendoza-Sassi ◽  
Obirajara Rodrigues ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Biopsy ◽  
Diagnostic Methods ◽  
Biopsy Specimens

scholarly journals Rapid colorimetric hybridization assay for detecting amplified Helicobacter pylori DNA in gastric biopsy specimens.

1996 ◽  
Vol 34 (3) ◽  
pp. 530-533 ◽  
Author(s):  
A P Lage ◽  
A Fauconnier ◽  
A Burette ◽  
Y Glupczynski ◽  
A Bollen ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Biopsy ◽  
Hybridization Assay ◽  
Biopsy Specimens

scholarly journals Optimal combination of media for primary isolation of Helicobacter pylori from gastric biopsy specimens.

1997 ◽  
Vol 35 (6) ◽  
pp. 1541-1544 ◽  
Author(s):  
R Piccolomini ◽  
G Di Bonaventura ◽  
D Festi ◽  
G Catamo ◽  
F Laterza ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Biopsy ◽  
Optimal Combination ◽  
Primary Isolation ◽  
Biopsy Specimens

Genotyping of Helicobacter pylori in paraffin-embedded gastric biopsy specimens: relation to histological parameters and effects on therapy

2002 ◽  
Vol 97 (7) ◽  
pp. 1687-1695 ◽  
Author(s):  
Ger H.A. Scholte ◽  
Leen-Jan Doorn ◽  
Annemieke Cats ◽  
Elisabeth Bloemena ◽  
Jan Lindeman ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Biopsy ◽  
Biopsy Specimens
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