Slime plugs do not inhibit surge flow in sieve tubes

Slime plugs, composed largely of P protein, on sieve plates in phloem of Heracleum mantegazzianum L. and Heracleum sphondylium Somm. and Lev. do not seem to be effective in preventing surge flow caused by loss of turgor, therefore calling into question the role of slime plugs in phloem.

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The Role of Cytokinin in Organised Differentiation of Vascular Tissues

Functional Plant Biology ◽

10.1071/pp9930601 ◽

1993 ◽

Vol 20 (5) ◽

pp. 601 ◽

Cited By ~ 10

Author(s):

R Aloni

Keyword(s):

Optimal Level ◽

Ipt Gene ◽

Vascular Differentiation ◽

Vascular Tissues ◽

Plant Body ◽

Vessel Maturation ◽

Sieve Tubes ◽

Sieve Plates ◽

Vessel Regeneration

The role of cytokinin as a limiting and controlling factor in the differentiation of vascular tissues in the plant body is discussed. Cytokinin controls the early stages of fibre differentiation in Helianthus stems and the regeneration of vessels and sieve tubes around a wound in Coleus internodes. The influence of cytokinin on cell differentiation in the vascular tissues varies according to its physiological levels and the levels of auxin. Cytokinin induces an acropetal polar pattern of vessel regeneration around a wound in internodes of Coleus. Similarly, adventitious roots induce acropetal polar patterns of vessel maturation in hypocotyls of Cucurbita. Cytokinin increases the sensitivity of the vascular cambium to the auxin stimulation, resulting in the highest ratio of phloem/xylem under the optimal level of cytokinin. High levels of cytokinin promote callose production on sieve plates. Studies of transgenic plants with altered levels of cytokinin (overexpressing the ipt gene) confirm the involvement of cytokinin in vascular differentiation.

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The role of auxin in the removal of dormancy callose and resumption of phloem activity in Vitis vinifera

Canadian Journal of Botany ◽

10.1139/b91-232 ◽

1991 ◽

Vol 69 (8) ◽

pp. 1825-1832 ◽

Cited By ~ 32

Author(s):

Roni Aloni ◽

Ayala Raviv ◽

Carol A. Peterson

Keyword(s):

Vitis Vinifera ◽

Key Words ◽

Radial Direction ◽

Fluorescent Dye ◽

Vitis Vinifera L ◽

Naphthaleneacetic Acid ◽

Bud Break ◽

Sieve Tubes ◽

Sieve Plates

During winter the phloem of Vitis vinifera L. is dormant and is characterized by heavy deposits of callose on the sieve plates and lateral sieve areas of the sieve tubes. After bud break, the maturing leaves stimulate a nonpolar breakdown of this dormancy callose along the branch axis in the internodes located both above and below the leaves. However, the pattern of callose degradation in the radial direction is polar. It proceeds in a centrifugal direction so that the sieve tubes near the cambium become free of callose first and those adjacent to the periderm last. The effect of the leaves on the removal of dormancy callose can be replaced by auxin. Application of naphthaleneacetic acid to either the top or basal ends of excised dormant branches resulted in the removal of callose from sieve tubes, usually in less than a week. The fluorescent dye fluorescein was used to test phloem reactivation. Both acropetal and basipetal fluorescein movement occurred in sieve tubes in branches that were pretreated for 1 week with auxin, while much less movement of fluorescein occurred in the control branches, which remained dormant. Fluorescein translocation was observed in sieve tubes that had a reduced amount of callose and were wider than 20 μm, but was not detected in the narrow sieve tubes (diameters less than 15 μm) located next to the cambium. The possible roles of auxin, ethylene, and cytokinin in controlling callose levels in the sieve tubes are discussed. Key words: auxin, callose, fluorescein, Vitis vinifera, phloem, dormancy.

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Studies on the Phloem Sealing Mechanism in Ricinus Fruit Stalks

Functional Plant Biology ◽

10.1071/pp9830561 ◽

1983 ◽

Vol 10 (6) ◽

pp. 561 ◽

Cited By ~ 3

Author(s):

J Kallarackal ◽

JA Milburn

Keyword(s):

Electron Microscopy ◽

Sieve Tube ◽

Sieve Plate ◽

Tube System ◽

Phloem Sap ◽

Starch Grains ◽

P Protein ◽

Sieve Tubes ◽

Sealing Mechanism ◽

Sieve Plates

Fruit stalks of R. communis were made to exude phloem sap by repeated slicing at intervals of a few minutes. Samples 1 mm thick from the fruit stalks were fixed for electron microscopy. Samples were also fixed and processed for electron microscopy from previously intact (non-exuding) fruit stalks. Examination of the sieve tubes from these two different samples showed predominantly open sieve-plate pores in the exuding fruit stalk. The sieve plates of the non-exuding fruit stalk showed occlusion of the sieve-plate pores by P-protein. The starch grains from the broken plastids also had characteristic distributions. The implications of these observations are discussed in relation to comprehending the mechanism by which sieve-plate pores become choked, and so sealing the sieve-tube system as a result of injury.

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Heracleum asperum M. B. Fl Heracleum leskovii A. Grossh Heracleum mantegazzianum Sommier & Levier Heracleum persicum Desf. ex Fisch Heracleum sibiricum L. Heracleum sosnowskyi Manden Heracleum sphondylium L. Heracleum wilhelmsii Fisch. & Ave-Lall Apiaceae

Ethnobotany of Mountain Regions ◽

10.1007/978-3-030-28940-9_71 ◽

2020 ◽

pp. 477-494

Author(s):

Rainer W. Bussmann ◽

Ketevan Batsatsashvili ◽

Zaal Kikvidze ◽

Seyed Hamed Moazzami Farida ◽

Abdolbaset Ghorbani ◽

...

Keyword(s):

Heracleum Sosnowskyi ◽

Heracleum Mantegazzianum ◽

Heracleum Persicum ◽

Heracleum Sphondylium

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A crystalline inclusion in sieve element nuclei of Amsinckia. II. The inclusion in maturing cells

Journal of Cell Science ◽

10.1242/jcs.38.1.11 ◽

1979 ◽

Vol 38 (1) ◽

pp. 11-22

Author(s):

K. Esau ◽

A.C. Magyarosy

Keyword(s):

Hydrostatic Pressure ◽

Sieve Element ◽

Crystalline Inclusion ◽

Sieve Elements ◽

P Protein ◽

Sudden Release ◽

Sieve Plates ◽

Tubular Components

The compounds crystalloids formed in sieve element nuclei of Amsinckia douglasiana A. DC. (Boraginaceae) during differentiation of the cell become disaggregated during the nuclear breakdown characteristic of a maturing sieve element. The phenomenon occurs in both healthy and virus-infected plants. The crystalloid component termed cy, which is loosely aggregated, separates from the densely aggregated component termed cx and disperses. The cx component may become fragmented, or broken into large pieces, or remain intact after the cell matures. After their release from the nucleus both crystalloid components become spatially associated with the dispersed P-protein originating in the cytoplasm, but remain distinguishable from it. The component tubules of P-protein are hexagonal in transections and are somewhat wider than the 6-sided cy tubules. The cx tubules are much narrower than the P-protein or the cy tubules and have square transections. Both the P-protein and the products of disintegrated crystalloids accumulate at sieve plates in sieve elements subjected to sudden release of hydrostatic pressure by cutting the phloem. The question of categorizing the tubular components of the nuclear crystalloid of a sieve element with reference to the concept of P-protein is discussed.

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A set of primers for plastid indels and nuclear microsatellites in the invasive plant Heracleum mantegazzianum (Apiaceae) and their transferability to Heracleum sphondylium

Molecular Ecology Resources ◽

10.1111/j.1471-8286.2007.01911.x ◽

2008 ◽

Vol 8 (1) ◽

pp. 161-163 ◽

Cited By ~ 5

Author(s):

P. HENRY ◽

J. PROVAN ◽

J. GOUDET ◽

A. GUISAN ◽

Š. JAHODOVÁ ◽

...

Keyword(s):

Invasive Plant ◽

Nuclear Microsatellites ◽

Heracleum Mantegazzianum ◽

Heracleum Sphondylium

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Fibronectin deficiency and intestinal transvascular fluid balance during bacteremia

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1982.242.4.h557 ◽

1982 ◽

Vol 242 (4) ◽

pp. H557-H564

Author(s):

B. C. Dillon ◽

T. M. Saba

Keyword(s):

Vascular Permeability ◽

Fluid Balance ◽

Protein Concentration ◽

Lymph Flow ◽

Fluid Flux ◽

Fluid Filtration ◽

Coated Particles ◽

P Protein ◽

Small Intestinal

Reticuloendothelial (RE) clearance dysfunction, which can be induced by opsonic fibronectin deficiency, has been correlated with organ failure during sepsis. We investigate the role of opsonic fibronectin deficiency and RE blockade in modulating alterations in intestinal transvascular fluid balance induced by Pseudomonas bacteremia using an isolated, innervated, and autoperfused canine small intestinal segment. Intravenous infusion of gelatin-coated particles was used to induce fibronectin deficiency and RE blockade. Lymph flow and lymph/plasma (L/P) protein concentration ratios were stable following intravenous challenge with bacteria or gelatin-coated particles. In contrast, lymph flow increased and L/P ratio decreased significantly when bacteremia coexisted with particle-induced opsonic fibronectin deficiency and RE blockade. This elevation in lymph flow and decline in L/P ratio was associated with normal vascular permeability to albumin, IgG, and IgM. The increase in intestinal fluid flux during bacteremia with RE blockade appears to be due to an increase in microvascular hydrostatic pressure and not to an increase in vascular permeability. These findings emphasize a potentially important role for fibronectin and associated RE system function as determinants of fluid filtration during sepsis.

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Interferon-Inducible GTPase 1 Impedes the Dimerization of Rabies Virus Phosphoprotein and Restricts Viral Replication

Journal of Virology ◽

10.1128/jvi.01203-20 ◽

2020 ◽

Vol 94 (21) ◽

Author(s):

Bin Tian ◽

Yueming Yuan ◽

Yu Yang ◽

Zhaochen Luo ◽

Baokun Sui ◽

...

Keyword(s):

Viral Replication ◽

Viral Infection ◽

Rabies Virus ◽

Parasite Infection ◽

Major Public Health Problem ◽

Intracellular Parasite ◽

Neurotropic Virus ◽

Pathogen Invasion ◽

P Protein

ABSTRACT Rabies, caused by rabies virus (RABV), is an ancient zoonosis and still a major public health problem for humans, especially in developing countries. RABV can be recognized by specific innate recognition receptors, resulting in the production of hundreds of interferon-stimulated genes (ISGs), which can inhibit viral replication at different stages. Interferon-inducible GTPase 1 (IIGP1) is a mouse-specific ISG and belongs to the immunity-related GTPases (IRGs) family. IIGP is reported to constrain intracellular parasite infection by disrupting the parasitophorous vacuole membrane. However, the role of IIGP1 in restricting viral replication has not been reported. In this present study, we found that IIGP1 was upregulated in cells and mouse brains upon RABV infection. Overexpression of IIGP1 limited RABV replication in cell lines and reduced viral pathogenicity in a mouse model. Consistently, deficiency of IIGP1 enhanced RABV replication in different parts of mouse brains. Furthermore, we found that IIGP1 could interact with RABV phosphoprotein (P protein). Mutation and immunoprecipitation analyses revealed that the Y128 site of P protein is critical for its interaction with IIGP1. Further study demonstrated that this interaction impeded the dimerization of P protein and thus suppressed RABV replication. Collectively, our findings for the first reveal a novel role of IIGP1 in restricting a typical neurotropic virus, RABV, which will provide fresh insight into the function of this mouse-specific ISG. IMPORTANCE Interferon and its downstream products, ISGs, are essential in defending against pathogen invasion. One of the ISGs, IIGP1, has been found to constrain intracellular parasite infection by disrupting their vacuole membranes. However, the role of IIGP1 in limiting viral infection is unclear. In this study, we show that infection with a typical neurotropic virus, RABV, can induce upregulation of IIGP1, which, in turn, suppresses RABV by interacting with its phosphoprotein (P protein) and thus blocking the dimerization of P protein. Our study provides the first evidence that IIGP1 functions in limiting viral infection and provides a basis for comprehensive understanding of this important ISG.

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Über den Zuckergehalt der Siebröhren- bzw. Siebzellensäfte von Heracleum Mantegazzianum und Picea abies (L.) KARST.

Zeitschrift für Naturforschung B ◽

10.1515/znb-1959-0414 ◽

1959 ◽

Vol 14 (4) ◽

pp. 278-281 ◽

Cited By ~ 26

Author(s):

Hubert Ziegler ◽

Tom E. Mittler

Keyword(s):

Picea Abies ◽

Sieve Tube ◽

Vascular Bundles ◽

Heracleum Mantegazzianum ◽

Sieve Tubes

Sieve-tube sap from the petioles of Heracleum Mantegazzianum and Picea abies stems was obtained by severing the proboscides of aphids tapping the sieve tubes of these plants. Sucrose was the only sugar detected in the sieve-tube sap, and occurred at concentrations of 24% (Heracleum) and 10% (w/v) (Picea). Volumes of sieve-tube sap equal to 5500 sieve-tube cells of Heracleum and 50 sievetube cells of Picea exuded from severed aphid probiscides.The mouth-parts of the aphids living within hollow Heracleum petioles normally penetrate the xylem of the vascular bundles in order to reach the phloem sieve-tubes. The aphids also tap the sievetubes of isolated phloem strands.

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