Identification and molecular characterization of a sex chromosome rearrangement causing a soft and pliable (spli) larval body phenotype in the silkworm, Bombyx mori

Genome ◽  
2010 ◽  
Vol 53 (1) ◽  
pp. 45-54 ◽  
Author(s):  
Tsuguru Fujii ◽  
Seigo Kuwazaki ◽  
Kimiko Yamamoto ◽  
Hiroaki Abe ◽  
Akio Ohnuma ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Sex Chromosome ◽  
Chromosome Rearrangement ◽  
Artificial Chromosome ◽  
Chromosome Breakage ◽  
Z Chromosome ◽  
Larval Body ◽  
Cytogenetic Analyses ◽  
Eye Pigmentation ◽  
Silkworm Bombyx Mori

We carried out genetic and cytogenetic analyses of X-ray-induced deleterious Z chromosomes that result in a soft and pliable (spli) phenotype in the silkworm, Bombyx mori . In a B. mori strain with a spli phenotype, we found the Z chromosome broken between the sch (1–21.5) and od (1–49.6) loci. We also found a chromosomal fragment bearing a fifth-chromosome locus for egg and eye pigmentation fused to a Z chromosome fragment. By means of fluorescence in situ hybridization using bacterial artificial chromosome clones as probes, we confirmed that the fused chromosome is composed of a fragment of chromosome 5 and a fragment of the Z chromosome. Moreover, a predicted gene, GA002017, the Bombyx ortholog of the Drosophila gene acj6 (Bmacj6), was completely deleted by the Z chromosome breakage event. The relationship between Bmacj6 and the spli phenotype is discussed.

scholarly journals Chromosomal fragment responsible for genetic mosaicism in larval body marking of the silkworm, Bombyx mori

1991 ◽  
Vol 57 (1) ◽  
pp. 11-16 ◽  
Author(s):  
Haruhiko Fujiwara ◽  
Osamu Ninaki ◽  
Masahiko Kobayashi ◽  
Jun Kusuda ◽  
Hideaki Maekawa
Keyword(s):  
Bombyx Mori ◽  
Short Chromosome ◽  
Larval Body ◽  
Chromosomal Fragment ◽  
X Ray ◽  
Chorion Genes ◽  
Genetic Mosaicism ◽  
Chromosome Fragments ◽  
Dna Fragment ◽  
Silkworm Bombyx Mori

SummarySeveral genetic mosaics for larval body marking of the silkworm, Bombyx mori, have been induced by X-ray irradiation. It is hypothesized that the occasional loss of chromosomal fragments carrying the genes for body marking during development may give rise to this type of mosaicism. Using pulsed field gel electrophoresis (PFGE), we find that a DNA molecule of about 2·5 megabases (Mb) is present in one type of mosaic (mottled striped strains pSm788 and pSm872), and not in any other strain. This DNA fragment hybridizes strongly with some chorion genes which are less than 6·9 cM away from the ps locus, and hence it corresponds to a chromosomal fragment containing genes for both striped marking (ps) and the chorion. In the non-mottled ps strain, the phenotype before X-ray irradiation, no band was detected either on a PFGE gel or after hybridization with the chorion probe. These results suggest that the mottled ps strains carry short chromosome fragments which are lost differentially during cell divisions.

Dosage analysis of Z chromosome genes using microarray in silkworm, Bombyx mori

2009 ◽  
Vol 39 (5-6) ◽  
pp. 315-321 ◽  
Author(s):  
Xingfu Zha ◽  
Qingyou Xia ◽  
Jun Duan ◽  
Chunyun Wang ◽  
Ningjia He ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Z Chromosome ◽  
Silkworm Bombyx Mori

scholarly journals A Dense Genetic Map of the Silkworm, Bombyx mori, Covering All Chromosomes Based on 1018 Molecular Markers

Genetics ◽  
1998 ◽  
Vol 150 (4) ◽  
pp. 1513-1525 ◽  
Author(s):  
Yuji Yasukochi
Keyword(s):  
Drosophila Melanogaster ◽  
Molecular Markers ◽  
Linkage Map ◽  
Bombyx Mori ◽  
Genetic Markers ◽  
Genetic Map ◽  
Z Chromosome ◽  
Linkage Groups ◽  
Average Interval ◽  
Silkworm Bombyx Mori

Abstract A dense linkage map was constructed for the silkworm, Bombyx mori, containing 1018 genetic markers on all 27 autosomes and the Z chromosome. Most of the markers, covering ∼2000 cM, were randomly amplified polymorphic DNAs amplified with primer-pairs in combinations of 140 commercially available decanucleotides. In addition, eight known genes and five visible mutations were mapped. Bombyx homologues of engrailed and invected genes were found to be closely linked, as in Drosophila melanogaster. The average interval between markers was ∼2 cM, equal to ∼500 kb. The correspondence of seven linkage groups to counterparts of the conventional linkage map was determined. This map is the first linkage map in insects having a large number of chromosomes (n = 28) that covers all chromosomes without any gaps.

Molecular analysis of sex chromosome-linked mutants in the silkworm Bombyx mori

2010 ◽  
Vol 89 (3) ◽  
pp. 365-374 ◽  
Author(s):  
Tsuguru Fujii ◽  
Hiroaki Abe ◽  
Toru Shimada
Keyword(s):  
Bombyx Mori ◽  
Molecular Analysis ◽  
Sex Chromosome ◽  
Silkworm Bombyx Mori

scholarly journals Mutation of a Cuticular Protein,BmorCPR2, Alters Larval Body Shape and Adaptability in Silkworm,Bombyx mori

Genetics ◽  
2014 ◽  
Vol 196 (4) ◽  
pp. 1103-1115 ◽  
Author(s):  
Liang Qiao ◽  
Gao Xiong ◽  
Ri-xin Wang ◽  
Song-zhen He ◽  
Jie Chen ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Body Shape ◽  
Cuticular Protein ◽  
Larval Body ◽  
Silkworm Bombyx Mori

scholarly journals End-sequencing and characterization of silkworm (Bombyx mori) bacterial artificial chromosome libraries

BMC Genomics ◽  
2007 ◽  
Vol 8 (1) ◽  
pp. 314 ◽  
Author(s):  
Yoshitaka Suetsugu ◽  
Hiroshi Minami ◽  
Michihiko Shimomura ◽  
Shun-ichi Sasanuma ◽  
Junko Narukawa ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Bombyx Mori ◽  
Artificial Chromosome ◽  
Silkworm Bombyx Mori

scholarly journals Effect of SSP – 11 on Weight and Tissue Composition of 4th Instar Larvae of Silkworm, Bombyx mori

2017 ◽  
Vol 11 ◽  
pp. 11
Author(s):  
K. Υenkata Rami Reddy ◽  
K. Sashindran Nair ◽  
S. Β. Magadum ◽  
R. Κ. Datta
Keyword(s):  
Bombyx Mori ◽  
Artificial Diet ◽  
Fat Body ◽  
Activity Levels ◽  
Tissue Composition ◽  
Fresh Weight ◽  
Total Proteins ◽  
Larval Body ◽  
Total Carbohydrates ◽  
Silkworm Bombyx Mori

The anti-juvenoid, SSP-11 ((E) 4-chloro-a, a, a-trifluro-N [ 1 (1 H-imidazole- 1 –y1)- 2-propoxy ethylyledene] O, toludine) was used to induce trimoulters from tetramoulter larvae of the silkworm, Bombyx mori (race NB7). The compound was fed for two days through an artificial diet at the concentration of 200 ppm to newly ecdysed 4th stadium larvae. Body and silkgland fresh weight were recorded daily in the 4th larval stadium and protein profiles of haemolymph, fat body and silkgland were determined at the end of the stadium. In larvae treated with SSP-11, feeding period in stadium 4 was prolonged by 3-4 days and 80-85% of the larvae started to spin as trimoulters. Eight and thirty fold increases in the larval body and silkgland weights respectively were the result of SSP-11. Further, total proteins, total carbohydrates, glycogen and nucleic acid (DNA & RNA) also increased. The activity levels of alanine aminotransferase and aspartate aminotransferasc increased significantly than in the control indicating increased mobilization of aminoacids into transamination activities.

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