The identification of restriction fragment length polymorphisms linked to seed colour genes in Brassica napus

Genome ◽  
1995 ◽  
Vol 38 (3) ◽  
pp. 534-542 ◽  
Author(s):  
A. E. Van Deynze ◽  
B. S. Landry ◽  
K. P. Pauls
Keyword(s):  
Brassica Napus ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Rflp Markers ◽  
Seed Colour ◽  
Dh Population ◽  
Length Polymorphisms ◽  
Dh Lines ◽  
Restriction Fragment Length

Restriction fragment length polymorphisms (RFLPs) linked to genes controlling seed colour were identified in rapeseed (Brassica napus). The efficiency of the RFLP analysis was enhanced by utilizing bulked segregant analysis, DNA clones that had previously been used to construct a RFLP map of B. napus, and a doubled-haploid (DH) population segregating for seed colour. Markers for two of the three seed colour genes segregating in the DH population were identified on the basis of χ2 analyses of marker distributions among visually classified black-, brown-, and yellow-seeded DH lines as well as ANOVA and quantitative trait locus analysis of light-reflectance measurements from seeds of the DH lines. The RFLP markers linked to seed colour that were identified in the present study will allow breeding strategies based on genotype selection to be developed for seed colour in rapeseed.Key words: RFLP markers, seed colour genes, rapeseed.

A genetic map for Brassica napus based on restriction fragment length polymorphisms detected with expressed DNA sequences

Genome ◽  
1991 ◽  
Vol 34 (4) ◽  
pp. 543-552 ◽  
Author(s):  
Benoit S. Landry ◽  
Nathalie Hubert ◽  
Takeomi Etoh ◽  
John J. Harada ◽  
Stephen E. Lincoln
Keyword(s):  
Brassica Napus ◽  
Genetic Map ◽  
Restriction Fragment ◽  
Dna Sequences ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Rflp Markers ◽  
Cdna Clones ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

F2 segregation analyses of DNA restriction fragment length polymorphisms (RFLPs) detected between two cultivars of canola ('Westar' × Topas') was used to construct a detailed genetic map of Brassica napus. RFLP markers were from a seedling-specific cDNA library. They were either randomly selected or previously characterized as seedling-specific cDNA clones. The 120 loci so far mapped in B. napus covered 1413 recombination units. They are assembled into 19 linkage groups. Seventeen loci were found unlinked to any other markers. Few polymorphisms were detected with the seedling-specific cDNAs and only seven loci could be mapped in this cross. Duplication of RFLP loci was extensive and reflects the amphidiploid nature of this species. However, several rearrangements of the linear order of duplicated loci could be seen. This RFLP study in B. napus provides important information on genome organization of functional DNA sequences and complements our current RFLP mapping effort in Brassica oleracea. The genetic markers of this map are currently being used in several breeding applications, such as tagging important agronomic traits and fingerprinting breeding lines and cultivars of canola, a major oilseed crop.Key words: genetic mapping, Brassica napus, breeding, restriction fragment length polymorphisms.

scholarly journals A genetic linkage map for the domesticated silkworm, Bombyx mori, based on restriction fragment length polymorphisms

1995 ◽  
Vol 66 (2) ◽  
pp. 109-126 ◽  
Author(s):  
Jinrui Shi ◽  
David G. Heckel ◽  
Marian R. Goldsmith
Keyword(s):  
Linkage Map ◽  
Bombyx Mori ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Genetic Maps ◽  
Crossing Over ◽  
Linkage Groups ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

SummaryWe present data for the initial construction of a molecular linkage map for the domesticated silkworm, Bombyx mori, based on 52 progeny from an F2 cross from a pair mating of inbred strains p50 and C108, using restriction fragment length polymorphisms (RFLPs). The map contains 15 characterized single copy sequences, 36 anonymous sequences derived from a follicular cDNA library, and 10 loci corresponding to a low copy number retrotransposon, mag. The 15 linkage groups and 8 ungrouped loci account for 23 of the 28 chromosomes and span a total recombination length of 413 cM; 10 linkage groups were correlated with established classic genetic maps. Scoring data from Southern blots were analysed using two Pascal programs written specifically to analyse linkage data in Lepidoptera, where females are the heterogametic sex and have achiasmatic meiosis (no crossing-over). These first examine evidence for linkage by calculating the maximum lod score under the hypothesis that the two loci are linked over the likelihood under the hypothesis that the two loci assort independently, and then determine multilocus linkage maps for groups of putatively syntenic loci by calculating the maximum likelihood estimate of the recombination fractions and the log likelihood using the EM algorithm for a specified order of loci along the chromosome. In addition, the possibility of spurious linkage was exhaustively tested by searching for genotypes forbidden by the absence of crossing-over in one sex.

Analysis of Restriction Fragment Length Polymorphisms in Rheumatic Diseases

1987 ◽  
Vol 13 (2) ◽  
pp. 353-367
Author(s):  
D. Olga McDaniel ◽  
Bruce O. Barger ◽  
John D. Reveille ◽  
Graciela S. Alarcón ◽  
William J. Koopman ◽  
...  
Keyword(s):  
Rheumatic Diseases ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Length Polymorphisms ◽  
Restriction Fragment Length
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