Biliary excretion of dye in dogs infused with BSP or its glutathione conjugate

A comparison of the maximal rates of biliary excretion (Tm), of dye in dogs infused with either BSP or its glutathione conjugate (BSP-GSH) was carried out. Tm was much higher when BSP-GSH rather than BSP was infused. This was accounted for by a significantly higher concentration of dye in bile of dogs receiving BSP-GSH. Evidence is presented that BSP and its conjugated metabolites compete for a common transport carrier and that BSP disproportionately depresses the biliary excretion of conjugated dye compounds. This latter observation accounts for the depressed dye Tm found during infusion of BSP. Choleresis invariably accompanied dye excretion. When BSP-GSH was infused, enhanced bile flow could be accounted for by the predicted osmotic activity of dye transported into bile. By contrast, the choleresis measured during infusion of BSP was significantly greater than that predicted. An additional mechanism for choleresis is operative, therefore, when unconjugated BSP is infused. Administration of taurocholate enhanced dye Tm when BSP-GSH was infused. Since increments of canalicular bile flow induced by theophylline and glucagon did not enhance dye excretion into bile, this effect by taurocholate appears to be related to taurocholate excretion per se rather than to the enhanced canalicular bile flow which accompanies its excretion.

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Relationship of Bile Salts and Bile Flow to Biliary Excretion of Iopanoic Acid

Investigative Radiology ◽

10.1097/00004424-197207010-00002 ◽

1972 ◽

Vol 7 (1) ◽

pp. 11-15

Author(s):

Albert A. Moss ◽

John R. Amberg ◽

Scott R. Jones

Keyword(s):

Biliary Excretion ◽

Bile Flow ◽

Bile Salts ◽

Iopanoic Acid ◽

Relationship Of

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The bile flow and biliary excretion of ursocholate in the rat

Life Sciences ◽

10.1016/0024-3205(83)90631-8 ◽

1983 ◽

Vol 33 (24) ◽

pp. 2377-2386 ◽

Cited By ~ 6

Author(s):

Kenichi Kitani ◽

Setsuko Kanai ◽

Yuko Sato ◽

Kiyoshisa Uchida

Keyword(s):

Biliary Excretion ◽

Bile Flow

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Influence of Canalicular Bile Flow on Sulfobromophthalein Transport Maximum in Bile in the Dog

The Liver. Quantitative Aspects of Structure and Function ◽

10.1159/000394823 ◽

2015 ◽

pp. 306-314 ◽

Cited By ~ 5

Author(s):

S. Erlinger ◽

M. Dumont

Keyword(s):

Bile Flow ◽

Canalicular Bile

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Intracellular alkalinization stimulates bile flow and vesicular-mediated exocytosis in IPRL

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1993.265.2.g347 ◽

1993 ◽

Vol 265 (2) ◽

pp. G347-G353 ◽

Cited By ~ 1

Author(s):

R. Bruck ◽

A. Benedetti ◽

M. Strazzabosco ◽

J. L. Boyer

Keyword(s):

Horseradish Peroxidase ◽

Biliary Excretion ◽

Bile Flow ◽

Channel Blocker ◽

Rat Hepatocytes ◽

Disulfonic Acid ◽

K Channel ◽

Rat Livers ◽

Intracellular Alkalinization ◽

Stimulation Of

Intracellular pH recovery from an acute alkaline load in rat hepatocytes is mediated by a Cl(-)-HCO3- exchanger, which is electroneutral, Na+ independent, and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) sensitive. Stimulation of this Cl(-)-HCO3- exchanger requires intact microtubules, suggesting that vesicular transport may be required to activate this exchanger. To determine if intracellular alkalinization stimulates biliary HCO3- excretion and bile flow in the intact liver by vesicle-mediated exocytosis, isolated perfused rat livers (IPRL) were alkalinized by two protocols. Isohydric changes in CO2 and HCO3- concentrations induced transient increases in bile flow by 36% (P < 0.01), which were abolished by DIDS (0.01 mM), inhibited by pretreatment with colchicine (P = 0.01), but not affected by membrane depolarization with the K(+)-channel blocker BaCl2 (1 mM). Similarly, perfusion with 20 mM NH4Cl produced a 42% increase in bile flow (P < 0.01) and a 26% increase in biliary HCO3- excretion. Both the increases in bile flow and HCO3- excretion were almost completely blocked by DIDS and inhibited by pretreatment with colchicine (P < 0.01). Biliary excretion of horseradish peroxidase was also increased during intracellular alkalinization with either protocol (P < 0.01). These findings suggest that intracellular alkalinization stimulates bile flow and biliary HCO3- excretion. Microtubule-dependent vesicular-mediated exocytosis is involved in this response.

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ENHANCED BILIARY THYROXINE EXCRETION IN RATS TREATED WITH PREGNENOLONE-16α-CARBONITRILE

Acta Endocrinologica ◽

10.1530/acta.0.0810110 ◽

1976 ◽

Vol 81 (1) ◽

pp. 110-119 ◽

Cited By ~ 4

Author(s):

M. M. Japundžić ◽

C. H. Bastomsky ◽

I. P. Japundžić

Keyword(s):

Thyroid Hormone ◽

Biliary Excretion ◽

Clearance Rate ◽

Bile Flow ◽

Stomach Tube ◽

Biliary Clearance ◽

Gunn Rats ◽

Peripheral Metabolism ◽

Hormonal Release ◽

Serum Tsh

ABSTRACT Normal rats were treated with pregnenolone-16α-carbonitrile (PCN) 10 mg/100 g by stomach tube twice daily for 3 days. In these animals the biliary excretion of intravenously injected 125I-thyroxine (T4) was enhanced and the bile: plasma 125I ratio (B/P ratio) and the biliary clearance rate of plasma 125I-T4 was increased. Normal rats were treated with PCN for 3 days and homozygous Gunn rats for 13 days. In both groups PCN enhanced the bile flow and elevated the B/P ratios and the biliary clearance rate of plasma T4 following ip injection of 125I-T4 17 h previously. PCN-treatment had no effect on the fractions of biliary 125I present as T4-glucuronide, T4 and I− in either the normal or Gunn rats. Treatment with PCN for 10 days produced goitres in normal and Gunn rats and in normal rats elevated the serum TSH (bioassay) levels and the 17 h thyroid 131I uptake as well as the serum PB125I concentrations, without affecting stable PBI concentrations. These data indicated increased pituitary TSH release in response to increased peripheral metabolism of thyroid hormone; enhanced hormonal release from the thyroid kept pace with the accelerated peripheral loss.

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