Convenient apparatus for recording contractions of isolated heart muscle

A simple and convenient apparatus for physiological or pharmacological experiments on preparations of isolated heart muscle is described. Provision is made for recording independently from two preparations mounted in the same bath. Electrodes for stimulation or recording are incorporated in the clamps that hold the tissue. The construction ensures the maintenance of a high oxygen tension at the surface of the tissue without the mechanical artifacts that result from bubbling oxygen directly past it. papillary muscle; isolated organ bath; isolated atria; double muscle preparation; circulating salt solution; stimulating electrodes; concentration-effect curves; inotropic and chronotropic effects; oxygenation Submitted on November 5, 1964

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The Function of Cytochrome b in a Soluble Nonphosphorylating Heart Muscle Preparation

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)97303-5 ◽

1965 ◽

Vol 240 (7) ◽

pp. 3165-3169

Author(s):

Joseph D. Shore ◽

W.W. Wainio

Keyword(s):

Cytochrome B ◽

Heart Muscle ◽

Muscle Preparation

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Isolated Papillary Muscle Preparation

Measurement of Cardiac Function ◽

10.1201/9781003071853-3 ◽

2020 ◽

pp. 41-59

Author(s):

Joseph M. Capasso

Keyword(s):

Papillary Muscle ◽

Muscle Preparation

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Identification of serotonergic (5HT1A-type) receptors in broiler small intestine by application of serotonin and its agonists and antagonists

Veterinarski glasnik ◽

10.2298/vetgl1102051m ◽

2011 ◽

Vol 65 (1-2) ◽

pp. 51-59 ◽

Cited By ~ 1

Author(s):

Indira Mujezinovic ◽

Vitomir Cupic ◽

Ahmed Smajlovic ◽

Mehmed Muminovic

Keyword(s):

Smooth Muscle ◽

Small Intestine ◽

Smooth Muscles ◽

Muscle Layer ◽

Force Transducer ◽

Mechanical Activity ◽

Organ Bath ◽

Pathological Conditions ◽

Isolated Organ Bath ◽

Longitudinal Layer

Serotonin or 5-hydroxytryptamine (5-HT), is a monoamine neurotransmitter synthesised from L-tryptophan in serotonergic neurons and enterochromaffin cells of the gastrointestinal tract. This neurotransmitter is widely distributed in the animal and plant kingdom and regulates some central and peripheral functions through several types of specific serotonergic (5-HT) receptors. Since it is known that the effect of serotonin, especially in pathological conditions, is very important, we believe that determining the types of receptors for this substance would make it possible to use their agonist or antagonists, which would undoubtedly enhance the pharmacotherapy of functional disruption of the small intestine in broilers. Investigations were carried out on isolated smooth muscle strips of the circular and longitudinal layer of the broiler small intestine (strip dimension 3-4 mm x 2 cm). The muscle strips were placed in an isolated organ bath. The mechanical activity of the preparations was recorded via an isotonic force transducer coupled to a pen recorder. This was done following the addition of serotonin (nonselective 5-HT agonist), 8-OH-DPAT (selective 5-HT1A agonist) and spiroxatrin (selective 5-HT1A antagonist). The sensitivity of the tissues to acetylcholine was tested before starting the experiments. Using the obtained results, it can be concluded that 5HT1A type receptors are present in smooth muscles of the broiler small intestine, duodenum and ileum, especially in the longitudinal smooth muscle layer which reacted with contractions even to low serotonin concentration (10-6), but not in the jejunum.

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