scholarly journals The Function of Cytochrome b in a Soluble Nonphosphorylating Heart Muscle Preparation

1965 ◽  
Vol 240 (7) ◽  
pp. 3165-3169
Author(s):  
Joseph D. Shore ◽  
W.W. Wainio
Keyword(s):  
Cytochrome B ◽  
Heart Muscle ◽  
Muscle Preparation

Convenient apparatus for recording contractions of isolated heart muscle

1965 ◽  
Vol 20 (4) ◽  
pp. 755-757 ◽  
Author(s):  
John R. Blinks
Keyword(s):  
Papillary Muscle ◽  
Heart Muscle ◽  
Isolated Heart ◽  
Organ Bath ◽  
Muscle Preparation ◽  
Isolated Atria ◽  
Double Muscle ◽  
Stimulating Electrodes ◽  
Chronotropic Effects ◽  
Isolated Organ Bath

A simple and convenient apparatus for physiological or pharmacological experiments on preparations of isolated heart muscle is described. Provision is made for recording independently from two preparations mounted in the same bath. Electrodes for stimulation or recording are incorporated in the clamps that hold the tissue. The construction ensures the maintenance of a high oxygen tension at the surface of the tissue without the mechanical artifacts that result from bubbling oxygen directly past it. papillary muscle; isolated organ bath; isolated atria; double muscle preparation; circulating salt solution; stimulating electrodes; concentration-effect curves; inotropic and chronotropic effects; oxygenation Submitted on November 5, 1964

STUDIES ON THE INHIBITION OF THE MITOCHONDRIAL ATP-ase BY REDUCTION OF THE RESPIRATORY CHAIN

1960 ◽  
Vol 38 (1) ◽  
pp. 1195-1214 ◽  
Author(s):  
W. Chefurka
Keyword(s):  
Electron Transport ◽  
Oxidative Phosphorylation ◽  
Respiratory Chain ◽  
Transport System ◽  
Heart Muscle ◽  
Rat Heart ◽  
Complete Inhibition ◽  
Muscle Preparation ◽  
Rat Heart Mitochondria ◽  
Stimulation Of

The effects of inhibition of the electron transport system on the stimulation of the ATP-ase reaction in liver and rat-heart mitochondria by dinitrophenol were studied. Cyanide at concentrations 10−3M and higher effectively reduced the stimulation of ATP-ase by dinitrophenol. A similar but less striking inhibition was observed for rat-heart sarcosomes. This ATP-ase reaction was also inhibited in the presence of 10−4M cyanide with glutamate, β-hydroxybutyrate, DPNH, and succinate as reductants of the respiratory chain. Anaerobiosis also caused a substantial decrease in the ATP-ase reaction. In all instances, complete inhibition of the ATP-ase reaction could not be achieved when the respiratory chain was reduced. The magnesium-stimulated ATP-ase of rat-heart sarcosomes, of aged mitochondria, and of the Keilin–Hartree heart-muscle preparation was insensitive to reduction of the carriers suggesting that this reaction may constitute only part of the total ATP-ase reaction. The compatability of the various mechanisms of oxidative phosphorylation with these results is discussed.

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