scholarly journals Self-Organization Phenomena in Embryonic Stem Cell-Derived Embryoid Bodies: Axis Formation and Breaking of Symmetry during Cardiomyogenesis

2012 ◽  
Vol 195 (5) ◽  
pp. 377-391 ◽  
Author(s):  
Christiane Fuchs ◽  
Matthias Scheinast ◽  
Waltraud Pasteiner ◽  
Sabine Lagger ◽  
Manuela Hofner ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Embryoid Bodies ◽  
Self Organization ◽  
Axis Formation

Tie-1-directed expression of Cre recombinase in endothelial cells of embryoid bodies and transgenic mice

2001 ◽  
Vol 114 (4) ◽  
pp. 671-676 ◽  
Author(s):  
E. Gustafsson ◽  
C. Brakebusch ◽  
K. Hietanen ◽  
R. Fassler
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Cre Recombinase ◽  
Embryoid Bodies ◽  
Lymphoid Cells ◽  
Adult Brain ◽  
Specific Gene

Tissue-specific gene inactivation using the Cre-loxP system has become an important tool to unravel functions of genes when the conventional null mutation is lethal. We report here the generation of a transgenic mouse line expressing Cre recombinase in endothelial cells. In order to avoid the production and screening of multiple transgenic lines we used embryonic stem cell and embryoid body technology to identify recombinant embryonic stem cell clones with high, endothelial-specific Cre activity. One embryonic stem cell clone that showed high Cre activity in endothelial cells was used to generate germline chimeras. The in vivo efficiency and specificity of the transgenic Cre was analysed by intercrossing the tie-1-Cre line with the ROSA26R reporter mice. At initial stages of vascular formation (E8-9), LacZ staining was detected in almost all cells of the forming vasculature. Between E10 and birth, LacZ activity was detected in most endothelial cells within the embryo and of extra-embryonic tissues such as yolk sac and chorioallantoic placenta. Ectopic expression of Cre was observed in approximately 12–20% of the adult erythroid, myeloid and lymphoid cells and in subregions of the adult brain. These results show that the tie-1-Cre transgenic strain can efficiently direct deletion of floxed genes in endothelial cells in vivo.

scholarly journals Identification of genes involved in VEGF-mediated vascular morphogenesis using embryonic stem cell-derived cystic embryoid bodies

2004 ◽  
Vol 84 (9) ◽  
pp. 1209-1218 ◽  
Author(s):  
Yin-Shan Ng ◽  
Markus Ramsauer ◽  
Robyn M B Loureiro ◽  
Patricia A D'Amore
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Embryoid Bodies ◽  
Vascular Morphogenesis

Activin B mediated induction of Pdx1 in human embryonic stem cell derived embryoid bodies

2007 ◽  
Vol 362 (3) ◽  
pp. 568-574 ◽  
Author(s):  
Ulrik Frandsen ◽  
Ann Dorte Porneki ◽  
Charlotte Floridon ◽  
Basem M. Abdallah ◽  
Moustapha Kassem
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Embryoid Bodies

scholarly journals Hydrophobic surfaces for enhanced differentiation of embryonic stem cell-derived embryoid bodies

2008 ◽  
Vol 105 (38) ◽  
pp. 14459-14464 ◽  
Author(s):  
B. Valamehr ◽  
S. J. Jonas ◽  
J. Polleux ◽  
R. Qiao ◽  
S. Guo ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Embryoid Bodies ◽  
Hydrophobic Surfaces
Sign in / Sign up

Export Citation Format

Share Document