scholarly journals A Case of Renal Primitive Neuroectodermal Tumor Confirmed by Fluorescence in situ Hybridization

2015 ◽  
Vol 8 (1) ◽  
pp. 205-211 ◽  
Author(s):  
Toshiki Etani ◽  
Taku Naiki ◽  
Ryosuke Ando ◽  
Keitaro Iida ◽  
Aya Naiki-Ito ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Primitive Neuroectodermal Tumor ◽  
Left Kidney ◽  
Final Diagnosis ◽  
Fine Needle Biopsy ◽  
Neuroectodermal Tumor ◽  
Transmission Electron ◽  
Resected Tumor

Primitive neuroectodermal tumor (PNET) is a member of the Ewing's sarcoma family of tumors (ESFT). We report a case of PNET in a 66-year-old male who presented with a large solid tumor within the parenchyma of the middle pole of the left kidney with metastases to the left adrenal gland and right ischium. A fine-needle biopsy was performed and showed a small round cell tumor. Results of immunohistochemical staining suggested this tumor belonged to ESFT. Preoperative VDC-IE (combined vincristine, doxorubicin and cyclophosphamide followed by another combination of ifosfamide and etoposide) chemotherapy and left radical nephrectomy and adrenalectomy were performed. The histopathological findings of the resected tumor were similar to those in the biopsy specimen, but the results of AE1/AE3 were different. For the diagnosis, fluorescence in situ hybridization was performed. Split signals of the EWSR1 gene were detected, and transmission electron microscopy showed neuroendocrine granules and microtubules. The final diagnosis of this tumor was PNET of the kidney.

Primitive Neuroectodermal Tumor of the Kidney Confirmed by Fluorescence In Situ Hybridization

1997 ◽  
Vol 21 (4) ◽  
pp. 461-468 ◽  
Author(s):  
Michael Sheaff ◽  
Aidan McManus ◽  
Irene Scheimberg ◽  
Andrew Paris ◽  
Janet Shipley ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Primitive Neuroectodermal Tumor ◽  
Neuroectodermal Tumor

Primitive neuroectodermal tumor of the kidney confirmed by fluorescence in situ hybridization

2007 ◽  
Vol 41 (1) ◽  
pp. 75-76 ◽  
Author(s):  
Seiichi Kato ◽  
Toshimi Takeuchi ◽  
Tomonari Asano ◽  
Yoshihito Ban ◽  
Tetsuya Yamada ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Primitive Neuroectodermal Tumor ◽  
Neuroectodermal Tumor

Primitive neuroectodermal tumor of the breast: Immunohistochemistry and fluorescence in situ hybridization

2007 ◽  
Vol 57 (8) ◽  
pp. 509-512 ◽  
Author(s):  
Gen Tamura ◽  
Shun-ichi Sasou ◽  
Shun Kudoh ◽  
Jun Kikuchi ◽  
Akira Ishikawa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Primitive Neuroectodermal Tumor ◽  
Neuroectodermal Tumor

scholarly journals Use of Fluorescence In Situ Hybridization (FISH) in Diagnosis and Tailored Therapies in Solid Tumors

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1864
Author(s):  
Natalia Magdalena Chrzanowska ◽  
Janusz Kowalewski ◽  
Marzena Anna Lewandowska
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Tissue Sample ◽  
Soft Tissue Sarcomas ◽  
Standard Technique ◽  
Final Diagnosis ◽  
Probe Type ◽  
Genomic Imbalances ◽  
Formalin Fixed Paraffin Embedded

Fluorescence in situ hybridization (FISH) is a standard technique used in routine diagnostics of genetic aberrations. Thanks to simple FISH procedure is possible to recognize tumor-specific abnormality. Its applications are limited to designed probe type. Gene rearrangements e.g., ALK, ROS1 reflecting numerous translocational partners, deletions of critical regions e.g., 1p and 19q, gene fusions e.g., COL1A1-PDGFB, genomic imbalances e.g., 6p, 6q, 11q and amplifications e.g., HER2 are targets in personalized oncology. Confirmation of genetic marker is frequently a direct indication to start specific, targeted treatment. In other cases, detected aberration helps pathologists to better distinguish soft tissue sarcomas, or to state a final diagnosis. Our main goal is to show that applying FISH to formalin-fixed paraffin-embedded tissue sample (FFPE) enables assessing genomic status in the population of cells deriving from a primary tumor or metastasis. Although many more sophisticated techniques are available, like Real-Time PCR or new generation sequencing, FISH remains a commonly used method in many genetic laboratories.

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