scholarly journals Use of Polarized Light in the Diagnosis of “Fuzz” Granuloma in the Conjunctiva of a Child

2021 ◽  
pp. 239-242
Author(s):  
Andrew R. Dorion ◽  
Gordon Crabtree ◽  
R. Patrick Dorion
Keyword(s):  
Microscopic Examination ◽  
Polarized Light ◽  
Synthetic Fiber ◽  
Resected Specimen ◽  
Foreign Material ◽  
Teddy Bear ◽  
Resected Tissue

Synthetic fiber granulomas, also known as “Teddy Bear” granulomas or “Fuzz Ball granulomas,” are usually seen in childhood. We present a case of a 5-year-old child with a “Fuzz Ball” granuloma of the conjunctiva. The resected specimen was processed routinely. The use of polarized light during microscopic examination of the resected tissue made the diagnosis evident due to the prominent birefringence of the synthetic foreign material fibers.

scholarly journals A Concise Review of Amyloidosis in Animals

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Moges Woldemeskel
Keyword(s):  
Microscopic Examination ◽  
Protein Misfolding ◽  
Polarized Light ◽  
Amyloid Deposit ◽  
Clinical Findings ◽  
Wild Animals ◽  
Concise Review ◽  
Underlying Causes ◽  
Congo Red Staining ◽  
Misfolding Diseases

Amyloidosis refers to a group of protein misfolding diseases characterized by deposition of a particular amyloid protein in various organs and tissues of animals and humans. Various types and clinical forms of amyloidosis, in which the pathology and pathogenesis is diverse depending upon the underlying causes and species affected, are reported in domestic and wild animals. The clinical findings are also quite variable consequent to the variation of the tissues and organs involved and the extent of functional disruption of the affected organs in various animal species. The affected organs may be enlarged and exhibit variable pallor grossly, or the amyloid deposit may be discernible only after microscopic examination of the affected tissues. Amyloid appears as a pale eosinophilic homogenous extracellular deposit in tissues. However, microscopic examination and Congo red staining with green birefringence under polarized light are needed to confirm amyloid and differentiate it from other apparently similar extracellular deposits such as collagen and fibrin. Identifying the type of amyloid deposit needs immunohistochemical staining, ultrastructural characterization of the amyloid fibril, and if feasible also genetic studies of the involved species for clinical and prognostic purposes. This paper provides a concise review of the occurrence of amyloidosis in domestic and wild animals.

scholarly journals Synthetic fiber from a teddy bear causing keratitis and conjunctival granuloma: case report

2011 ◽  
Vol 11 (1) ◽  
Author(s):  
Mohammed K Farooq ◽  
Jan U Prause ◽  
Steffen Heegaard
Keyword(s):  
Case Report ◽  
Synthetic Fiber ◽  
Teddy Bear

Compound Odontoma in Three Dogs

2003 ◽  
Vol 20 (2) ◽  
pp. 79-83 ◽  
Author(s):  
Cláudia Ronca Felizzola ◽  
Marília Trierveiler Martins ◽  
Ângelo Stopiglia ◽  
Ney Soares de Araújo ◽  
Suzana Orsini Machado de Sousa
Keyword(s):  
Microscopic Examination ◽  
Young Female ◽  
Stage Iii ◽  
Resective Surgery ◽  
Resected Tissue ◽  
Compound Odontoma

Three young, female dogs were operated for compound odontoma. All tumors were considered stage III with treatment consisting of partial mandibulectomy or maxillectomy. Microscopic examination of the resected tissue confirmed the diagnosis. Relatively aggressive, resective surgery resulted in prolonged tumor-free intervals.

A new method for the preparation of thin sections of clays

1940 ◽  
Vol 25 (169) ◽  
pp. 529-533 ◽  
Author(s):  
A. V. Weatherhead
Keyword(s):  
Microscopic Examination ◽  
Polarized Light ◽  
Microscopic Investigation ◽  
New Method ◽  
Thin Sections

Thin sections of clays are not easily prepared by the routine petrographic methods. The chief difficulties are due to the extremely fine texture of the clays and to the soft and plastic nature of these materials. These difficulties are only too well known, and, although in some cases they have been partially overcome by impregnation in vacuo, it is safe to assume that really little work has been accomplished in the microscopic examination of clays on account of the difficulty of producing satisfactory thin sections. The writer has attempted to extend the scope of the microscopic investigation of clay rocLs by means of the technique outlined below.The method has been devised in response to an appeal by a friend, who, working in a laboratory at a well-known pottery works and thus connected with the clay industry, desired a method that would make possible the examination of thin sections of clays under high powers with polarized light.

Clinicopathologic Features of Two Rare Cases of Dedifferentiated Adamantinomas, Including Diagnostic Implications

2018 ◽  
Vol 27 (2) ◽  
pp. 193-202 ◽  
Author(s):  
Bharat Rekhi ◽  
Ayushi Sahay ◽  
Ajay Puri
Keyword(s):  
Microscopic Examination ◽  
Correct Diagnosis ◽  
Epithelial Differentiation ◽  
Resected Specimen ◽  
Intense Staining ◽  
Metastatic Lesions ◽  
First Case ◽  
Mitotic Figures ◽  
Aggressive Clinical Course

A dedifferentiated adamantinoma is a rare subtype of an adamantinoma, associated with a relatively aggressive clinical course, with less than 10 such cases reported so far. A 25-year-old-male presented with pain in his right leg of 1-year duration. Imaging disclosed a well-defined lytic, destructive lesion in his proximal tibia with a cortical break and a soft tissue component. Microscopic examination of the biopsy and resected specimen showed nests and clusters of atypical epithelial cells, along with significant areas showing markedly pleomorphic and spindly sarcomatous cells with interspersed mitotic figures and areas of stromal hyalinization. By immunohistochemistry, the areas of epithelial differentiation showed intense positivity for cytokeratin and p63, whereas the sarcomatous areas showed reduced to absent immunostaining. A 51-year-old lady presented with a recurrent tumor in her right tibia, which was initially diagnosed as an adamantinoma, along with metastatic lesions in her lung. Microscopic examination of the recurrent and metastatic tumors showed areas of epithelial differentiation along with spindly sarcomatous cells, arranged in fascicles. By immunohistochemistry, the areas of epithelial differentiation showed positivity for pan cytokeratin. Additionally, p63 was diffusely positive. p53 showed diffuse and intense staining pattern in the sarcomatous component (dedifferentiation). While the first case is disease-free, the second case is on follow-up. The 2 cases of dedifferentiated adamantinoma further confirm the rare occurrence of this tumor in our population. Its correct diagnosis has treatment implications. Differential diagnoses and literature review of similar reported cases are also presented in this article.

scholarly journals Synthetic fiber "Teddy Bear" conjunctival granuloma; A case report

2015 ◽  
Vol 10 (3) ◽  
pp. 342 ◽  
Author(s):  
Roshanak Aliakbar-Navahi ◽  
MohammadHossein Roozitalab ◽  
MohammadJavad Ashraf ◽  
Afsoon Hakimzadeh
Keyword(s):  
Case Report ◽  
Synthetic Fiber ◽  
Teddy Bear

Collagen-Rich Crystalloids in a Scarred Vascularized Cornea

2005 ◽  
Vol 129 (9) ◽  
pp. 1179-1182
Author(s):  
Natalie A. Afshari ◽  
Tarra M. Wright ◽  
Thomas J. Cummings ◽  
Alan D. Proia
Keyword(s):  
Salivary Gland ◽  
Alcian Blue ◽  
Microscopic Examination ◽  
Penetrating Keratoplasty ◽  
Corneal Stroma ◽  
Polarized Light ◽  
Myoepithelial Differentiation ◽  
Elastic Stain ◽  
Methenamine Silver ◽  
Hematoxylin Eosin

Abstract Collagen-rich crystalloids, also referred to as collagenous crystalloids, are uncommon findings in benign salivary gland tumors with myoepithelial differentiation and in cutaneous neoplasms. Herein, we report the presence of collagen-rich crystalloids in the scarred, vascularized cornea of a 56-year-old woman. The patient underwent penetrating keratoplasty, and microscopic examination of hematoxylin-eosin–stained sections disclosed large aggregates of refractile material within the corneal stroma. The deposits were partially birefringent when viewed with polarized light and composed of radially arranged columns (long rectangles) with rounded to pointed tips. The deposits had tinctorial properties of collagen using Masson trichrome and the van Gieson method for collagen, and they stained with Alcian blue, pH 2.5, and Verhoeff elastic stain. They did not stain with Gomori methenamine silver, Snook reticulin stain, or tyrosine using the Baker modification of the Millon reaction. This is the first report, to our knowledge, of collagen-rich crystalloids in the cornea. Their presence in the cornea indicates that these structures may occur in the absence of neoplasia.

High Voltage Electron Microscopy of Ion-Milled Dental Enamel

1974 ◽  
Vol 32 ◽  
pp. 60-61
Author(s):  
L. D. Ackerman ◽  
S. H. Y. Wei
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Third Molar ◽  
Polarized Light ◽  
Dental Enamel ◽  
Longitudinal Section ◽  
Ion Milling ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron ◽  
High Voltage Electron

Mature human dental enamel has presented investigators with several difficulties in ultramicrotomy of specimens for electron microscopy due to its high degree of mineralization. This study explores the possibility of combining ion-milling and high voltage electron microscopy as a means of circumventing the problems of ultramicrotomy.A longitudinal section of an extracted human third molar was ground to a thickness of about 30 um and polarized light micrographs were taken. The specimen was attached to a single hole grid and thinned by argon-ion bombardment at 15° incidence while rotating at 15 rpm. The beam current in each of two guns was 50 μA with an accelerating voltage of 4 kV. A 20 nm carbon coating was evaporated onto the specimen to prevent an electron charge from building up during electron microscopy.

Sign in / Sign up

Export Citation Format

Share Document