Comparison of Polarized and Depolarized Arrest in the Isolated Rat Heart for Long-term Preservation

Circulation ◽  
1997 ◽  
Vol 96 (9) ◽  
pp. 3148-3156 ◽  
Author(s):  
A. K. Snabaitis ◽  
M. J. Shattock ◽  
D. J. Chambers
Keyword(s):  
Rat Heart ◽  
Isolated Rat Heart ◽  
Long Term Preservation ◽  
Isolated Rat

Long-term prenylamine therapy: Effects on responses to myocardial ischaemia in the isolated rat heart

1982 ◽  
Vol 77 (1) ◽  
pp. 33-38 ◽  
Author(s):  
Allan S. Manning ◽  
Michael J. Shattock ◽  
Steven C. Dennis ◽  
David J. Hearse
Keyword(s):  
Myocardial Ischaemia ◽  
Rat Heart ◽  
Isolated Rat Heart ◽  
Isolated Rat

Effect of Hydrogen Sulfide on Reactions of Isolated Rat Heart under Volume Load and Ischemia-Reperfusion

2013 ◽  
Vol 4 (3) ◽  
pp. 201-212
Author(s):  
Tetyana V Shimanskaya ◽  
Yulia V. Goshovska ◽  
Olena M. Semenykhina ◽  
Vadim F. Sagach
Keyword(s):  
Hydrogen Sulfide ◽  
Rat Heart ◽  
Ischemia Reperfusion ◽  
Isolated Rat Heart ◽  
Volume Load ◽  
Isolated Rat

‘Cross Talk’ Between Opioid Peptide and Adrenergic Receptor Signaling in Isolated Rat Heart

Circulation ◽  
1997 ◽  
Vol 95 (8) ◽  
pp. 2122-2129 ◽  
Author(s):  
Salvatore Pepe ◽  
Rui-Ping Xiao ◽  
Charlene Hohl ◽  
Ruth Altschuld ◽  
Edward G. Lakatta
Keyword(s):  
Cross Talk ◽  
Rat Heart ◽  
Adrenergic Receptor ◽  
Opioid Peptide ◽  
Isolated Rat Heart ◽  
Receptor Signaling ◽  
Isolated Rat

scholarly journals Correction to: Peculiar Aspects in Influence of α1-Adrenoceptor Stimulation on Isolated Rat Heart

2021 ◽  
Author(s):  
T. L. Zefirov ◽  
I. I. Khabibrakhmanov ◽  
N. I. Ziyatdinova ◽  
A. L. Zefirov
Keyword(s):  
Rat Heart ◽  
Isolated Rat Heart ◽  
Isolated Rat

Dobutamine responsiveness, PET mismatch, and lack of necrosis in low-flow ischemia: is this hibernation in the isolated rat heart?

2003 ◽  
Vol 285 (1) ◽  
pp. H316-H324 ◽  
Author(s):  
Richard Southworth ◽  
Pamela B. Garlick
Keyword(s):  
Rat Heart ◽  
Recovery Of Function ◽  
Electron Microscopic Examination ◽  
Left Ventricular ◽  
Isolated Rat Heart ◽  
Low Flow ◽  
Hibernating Myocardium ◽  
Heart Model ◽  
Electron Microscopic ◽  
Isolated Rat

The clinical hallmarks of hibernating myocardium include hypocontractility while retaining an inotropic reserve (using dobutamine echocardiography), having normal or increased [18F]fluoro-2-deoxyglucose-6-phosphate (18FDG6P) accumulation associated with decreased coronary flow [flow-metabolism mismatch by positron emission tomography (PET)], and recovering completely postrevascularization. In this study, we investigated an isolated rat heart model of hibernation using experimental equivalents of these clinical techniques. Rat hearts ( n = 5 hearts/group) were perfused with Krebs-Henseleit buffer for 40 min at 100% flow and 3 h at 10% flow and reperfused at 100% flow for 30 min (paced at 300 beats/min throughout). Left ventricular developed pressure fell to 30 ± 8% during 10% flow and recovered to 90 ± 7% after reperfusion. In an additional group, this recovery of function was found to be preserved over 2 h of reperfusion. Electron microscopic examination of hearts fixed at the end of the hibernation period demonstrated a lack of ischemic injury and an accumulation of glycogen granules, a phenomenon observed clinically. In a further group, hearts were challenged with dobutamine during the low-flow period. Hearts demonstrated an inotropic reserve at the expense of increased lactate leakage, with no appreciable creatine kinase release. PET studies used the same basic protocol in both dual- and globally perfused hearts (with 250MBq18FDG in Krebs buffer ± 0.4 mmol/l oleate). PET data showed flow-metabolism “mismatch;” whether regional or global,18FDG6P accumulation in ischemic tissue was the same as (glucose only) or significantly higher than (glucose + oleate) control tissue (0.023 ± 0.002 vs. 0.011 ± 0.002 normalized counts · s-1· g-1· min-1, P < 0.05) despite receiving 10% of the flow. This isolated rat heart model of acute hibernation exhibits many of the same characteristics demonstrated clinically in hibernating myocardium.

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