Ca2+ Accumulation in Isolated Rat Heart Mitochondria under Maintenance of Mitochondrial Membrane Potential

2016 ◽  
Vol 7 (4) ◽  
pp. 309-319
Author(s):  
Alina Yu. Budko ◽  
Nataliya A. Strutynska ◽  
Iryna Yu. Okhay ◽  
Olena M. Semenykhina ◽  
Vadim F. Sagach
Keyword(s):  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Rat Heart ◽  
Mitochondrial Membrane ◽  
Isolated Rat Heart ◽  
Heart Mitochondria ◽  
Rat Heart Mitochondria ◽  
Isolated Rat

Effects of hydrogen peroxide and hypochlorite on membrane potential of mitochondria in situ in rat heart cells

1991 ◽  
Vol 69 (11) ◽  
pp. 1705-1712 ◽  
Author(s):  
Noburu Konno ◽  
K. J. Kako
Keyword(s):  
Hydrogen Peroxide ◽  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Rat Heart ◽  
Mitochondrial Membrane ◽  
Isolated Rat Heart ◽  
Heart Mitochondria ◽  
High Concentrations ◽  
Isolated Rat

Hydrogen peroxide (H2O2) and hypochlorite (HOCl) cause a variety of cellular dysfunctions. In this study we examined the effects of these agents on the electrical potential gradient across the inner membrane of mitochondria in situ in isolated rat heart myocytes. Myocytes were prepared by collagenase digestion and incubated in the presence of H2O2 or HOCl. Transmembrane electrical gradients were measured by distribution of [3H]triphenylmethylphosphonium+, a lipophilic cation. The particulate fraction was separated from the cytosolic compartment first by permeabilization using digitonin, followed by rapid centrifugal sedimentation through a bromododecane layer. We found that the mitochondrial membrane potential (161 ± 7 mV, negative inside) was relatively well maintained under oxidant stress, i.e., the potential was decreased only at high concentrations of HOCl and H2O2 and gradually with time. The membrane potential of isolated rat heart mitochondria was affected similarly by H2O2 and HOCl in a concentration- and time-dependent manner. High concentrations of oxidants also reduced the cellular ATP level but did not significantly change the matrix volume. When the extra-mitochondrial free calcium concentration was increased in permeabilized myocytes, the transmembrane potential was decreased proportionally, and this decrease was potentiated further by H2O2. These results support the view that heart mitochondria are equipped with well-developed defense mechanisms against oxidants, but the action of H2O2 on the transmembrane electrical gradient is exacerbated by an increase in cytosolic calcium. Keywords: ATP, calcium, cardiomyocyte, cell defense, mitochondrial membrane potential, oxidant, triphenylmethylphosphonium.

Stabilization of mitochondrial membrane potential prevents doxorubicin-induced cardiotoxicity in isolated rat heart

2010 ◽  
Vol 244 (3) ◽  
pp. 300-307 ◽  
Author(s):  
David Montaigne ◽  
Xavier Marechal ◽  
Riadh Baccouch ◽  
Thomas Modine ◽  
Sebastien Preau ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Rat Heart ◽  
Mitochondrial Membrane ◽  
Isolated Rat Heart ◽  
Isolated Rat

Effects of pinacidil and calcium on isolated rat heart mitochondria

2012 ◽  
Vol 443 (1) ◽  
pp. 113-117 ◽  
Author(s):  
S. M. Korotkov ◽  
L. V. Emel’yanova ◽  
I. V. Brailovskaya ◽  
V. P. Nesterov
Keyword(s):  
Rat Heart ◽  
Isolated Rat Heart ◽  
Heart Mitochondria ◽  
Rat Heart Mitochondria ◽  
Isolated Rat

Uncoupling and Antioxidant Effects of Ursolic Acid in Isolated Rat Heart Mitochondria

2011 ◽  
Vol 74 (7) ◽  
pp. 1640-1644 ◽  
Author(s):  
Julius Liobikas ◽  
Daiva Majiene ◽  
Sonata Trumbeckaite ◽  
Lolita Kursvietiene ◽  
Ruta Masteikova ◽  
...  
Keyword(s):  
Ursolic Acid ◽  
Rat Heart ◽  
Isolated Rat Heart ◽  
Heart Mitochondria ◽  
Rat Heart Mitochondria ◽  
Antioxidant Effects ◽  
Isolated Rat

scholarly journals Celecoxib Decreases Mitochondrial Complex IV Activity and Induces Oxidative Stress in Isolated Rat Heart Mitochondria: An Analysis for its Cardiotoxic Adverse Effect

2020 ◽  
Author(s):  
Saman Atashbar ◽  
Elham Mohammad Khanlou ◽  
Saleh Khezri ◽  
Peyman Kurdpour ◽  
Ahmad Salimi
Keyword(s):  
Lipid Peroxidation ◽  
Rat Heart ◽  
Isolated Rat Heart ◽  
Mitochondrial Swelling ◽  
Heart Mitochondria ◽  
Isolated Mitochondria ◽  
Biochemical Assays ◽  
Ros Formation ◽  
Rat Heart Mitochondria ◽  
Isolated Rat

Abstract Background In spite of the cardiotoxic effect of selective cyclooxygenase-2 inhibitors, they are most widely used as anti-inflammatory and analgesic drugs. Today, valdecoxib and rofecoxib have been withdrawn on the market but celecoxib remains. In this study, we focused on an analysis of celecoxib toxic effects on isolated mitochondrial. Methods isolated rat heart mitochondria were obtained using differential centrifugation. Using flowcytometry and biochemical assays we searched succinate dehydrogenases (SDH), mitochondrial membrane potential (MMP), reactive oxygen species (ROS) formation, mitochondrial swelling, lipid peroxidation and mitochondrial complexes activity in rat heart isolated mitochondria. Results In here our results indicated a significant decrease in activity of complexes IV after exposure with celecoxib (16 µg/ml). This decrease in activity of complexes IV is paralleled by the MMP collapse, ROS formation, mitochondrial swelling and lipid peroxidation. Conclusion For the first time, this introductory study has showed a significant decrease in activity of complexes IV and mitochondrial dysfunction after exposure with celecoxib in rat heart isolated mitochondria.

scholarly journals Actions of Nitric Oxide Synthase Inhibitors on Mitochondrial Respiration in Isolated Rat Heart Mitochondria

2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
Monica H. Dholakia ◽  
Siva SVP. Sakamuri ◽  
Venkata N. Sure ◽  
Jared A. Sperling ◽  
Ryousuke Satou ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Mitochondrial Respiration ◽  
Rat Heart ◽  
Isolated Rat Heart ◽  
Heart Mitochondria ◽  
Nitric Oxide Synthase Inhibitors ◽  
Rat Heart Mitochondria ◽  
Oxide Synthase ◽  
Isolated Rat
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