Chronic Perilymphatic Fistula: Experimental Model in the Guinea Pig

1988 ◽  
Vol 99 (4) ◽  
pp. 380-388 ◽  
Author(s):  
Paul Flint ◽  
Larry G. Duckert ◽  
Robert A. Dobie ◽  
Edwin W. Rubel
Keyword(s):  
Guinea Pig ◽  
Hair Cell ◽  
Round Window ◽  
Cell Loss ◽  
Hair Cell Loss ◽  
Scala Tympani ◽  
Rubber Tubing ◽  
Perilymphatic Fistula ◽  
Similar Threshold ◽  
Baseline Levels

Chronic perilymphatic fistulas were created in guinea pig cochleas using silicone rubber tubing placed into the scala tympani through the round window. Fistula patency was determined by fluorescein perfusion into cerebral spinal fluid. Fistulas were found to be patent in 6 of 6 animals at 7 days and 8 of 13 animals at 28 days. Analysis of ABRs revealed threshold increases of 10 to 15 dB across all frequencies at 1 hour and 7 days. However, thresholds returned to pre-fistula levels by 28 days. Animals with acute fistulas (simple laceration of the round window) had similar threshold increases at 1 hour; however, recovery to baseline levels occurred by day 7. Control animals with intact round windows did not have threshold shifts. Scanning electron microscopy revealed hair cell loss localized to the apical and basal turns of the cochlea. The morphologic changes observed occurred acutely (within 7 days) and were not progressive, despite the presence of a fistula. Hair cell loss or degeneration did not correlate with hearing loss.

Hair Cell Loss in the Aged Guinea Pig Cochlea

1999 ◽  
Vol 119 (1) ◽  
pp. 42-47 ◽  
Author(s):  
Neil J. Ingham, Spiro D. Comis, Deb
Keyword(s):  
Guinea Pig ◽  
Hair Cell ◽  
Cell Loss ◽  
Hair Cell Loss ◽  
Guinea Pig Cochlea

Effects of lonomer Cement on Guinea Pig Cochleas

1997 ◽  
Vol 76 (4) ◽  
pp. 223-230 ◽  
Author(s):  
Taisuke Kobayashi ◽  
Yasuyuki Hinohira ◽  
Masamitsu Hyodo ◽  
Goran Bredberg ◽  
Edel Alsterborg
Keyword(s):  
Hearing Loss ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Morphological Study ◽  
Round Window ◽  
Cell Loss ◽  
Round Window Membrane ◽  
Hair Cell Loss ◽  
Ear Surgery ◽  
Hearing Thresholds

The inner ear toxicity of ionomeric cement (lonocem®) when used as a reconstructive material in ear surgery was evaluated in 47 guinea pigs used in a morphological study. The cement was implanted on either the promontory, the round window membrane or the stapes. There was no significant hair cell loss compared with the control ear at three weeks, two months or three months after implantation. Using electrocochleography, the hearing thresholds before, and three weeks, two and three months after the implantation were compared in an additional seven animals. No evident hearing loss was observed in any animal during the study period. No morphological or functional evidence for an ototoxic effect of the ionomeric cement was found in this study.

Idiopathic hair cell loss in the guinea pig

1978 ◽  
Vol 221 (1) ◽  
pp. 7-13 ◽  
Author(s):  
Libuše Úlehlová ◽  
L. Voldřich
Keyword(s):  
Guinea Pig ◽  
Hair Cell ◽  
Cell Loss ◽  
Hair Cell Loss

Permanent Threshold Shift and Cochlear Hair Cell Loss in the Kanamycin-Treated Guinea Pig

1978 ◽  
Vol 86 (6) ◽  
pp. ORL-886-ORL-887 ◽  
Author(s):  
Cynthia A. Prosen ◽  
Michael R. Petersen ◽  
David. B. Moody ◽  
William C. Stebbins ◽  
Joseph E. Hawkins
Keyword(s):  
Guinea Pig ◽  
Hair Cell ◽  
Hair Cells ◽  
Guinea Pigs ◽  
Cell Loss ◽  
Outer Hair Cells ◽  
Hair Cell Loss ◽  
Hearing Sensitivity ◽  
Cochlear Hair Cell ◽  
Differential Contribution

The differential contribution of the inner hair cells (IHC) and the outer hair cells (OHC) in the mammalian cochlea to hearing sensitivity was assessed in six behaviorally-trained guinea pigs by comparing audiograms preadministration and postadministration of kanamycin, an antibiotic that predominantly destroys guinea pig OHC while leaving the IHC structurally unchanged. The results support the hypothesis that only the IHC of the cochlea responds to tones approximately 50 to 60 dB above the threshold of the intact cochlea.

Round Window Application of D-Methionine Provides Complete Cisplatin Otoprotection

2002 ◽  
Vol 126 (6) ◽  
pp. 683-689 ◽  
Author(s):  
Kurtis D. Korver ◽  
Leonard P. Rybak ◽  
Craig Whitworth ◽  
Kathleen M. Campbell
Keyword(s):  
Hearing Loss ◽  
Hair Cell ◽  
Outer Hair Cell ◽  
Round Window ◽  
Organ Of Corti ◽  
Cell Loss ◽  
Auditory Brainstem Responses ◽  
Round Window Membrane ◽  
Hair Cell Loss ◽  
Minute Amount

OBJECTIVE: Cisplatin is a widely used, very effective chemotherapeutic agent that can cause severe ototoxicity. In this study, D-methionine was tested as an otoprotectant via round window membrane (RWM) application in the chinchilla. METHODS: A minute amount of cisplatin alone, or D-methionine followed by cisplatin, was applied topically directly to the intact RWM of anesthetized adult chinchillas. Auditory brainstem responses were measured before and 1 week after topical round window application. Animals were killed, and the cochleas were examined. RESULTS: The ears pretreated with D-methionine were completely protected from hearing loss and hair cell loss in the organ of Corti compared with controls. The ears receiving cisplatin without D-methionine protection sustained nearly complete hearing loss with threshold shifts of >60 dB, with extensive outer hair cell loss throughout the organ of Corti but particularly in the basal turn. CONCLUSION: These results demonstrate that topical D-methionine provides excellent otoprotection against cisplatin-induced ototoxicity both electrophysiologically and structurally.

Quercetin protects against hair cell loss in the zebrafish lateral line and guinea pig cochlea

2016 ◽  
Vol 342 ◽  
pp. 80-85 ◽  
Author(s):  
Yoshinobu Hirose ◽  
Kazuma Sugahara ◽  
Eiju Kanagawa ◽  
Yousuke Takemoto ◽  
Makoto Hashimoto ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Hair Cell ◽  
Lateral Line ◽  
Cell Loss ◽  
Hair Cell Loss ◽  
Guinea Pig Cochlea
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