Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation

2015 ◽  
Vol 43 (1) ◽  
pp. 9-18 ◽  
Author(s):  
Jovana Jasnic-Savovic ◽  
Andrijana Klajn ◽  
Milena Milivojevic ◽  
Marija Mojsin ◽  
Gordana Nikcevic
Keyword(s):  
Neural Differentiation ◽  
In Vitro Model ◽  
Embryonal Carcinoma ◽  
Model System ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Serum Free ◽  
Vitro Model ◽  
Serum Free Conditions

scholarly journals Exposure of ichnovirus particles to digitonin leads to enhanced infectivity and induces fusion from without in an in vitro model system

2007 ◽  
Vol 88 (11) ◽  
pp. 2977-2984 ◽  
Author(s):  
Don Stoltz ◽  
Renée Lapointe ◽  
Andrea Makkay ◽  
Michel Cusson
Keyword(s):  
Outer Membrane ◽  
In Vitro Model ◽  
Model System ◽  
Host Cells ◽  
Fusion Event ◽  
Susceptible Host ◽  
In Vitro Model System ◽  
Vitro Model

Unlike most viruses, the mature ichnovirus particle possesses two unit membrane envelopes. Following loss of the outer membrane in vivo, nucleocapsids are believed to gain entry into the cytosol via a membrane fusion event involving the inner membrane and the plasma membrane of susceptible host cells; accordingly, experimentally induced damage to the outer membrane might be expected to increase infectivity. Here, in an attempt to develop an in vitro model system for studying ichnovirus infection, we show that digitonin-induced disruption of the virion outer membrane not only increases infectivity, but also uncovers an activity not previously associated with any polydnavirus: fusion from without.

Neural differentiation arrest in embryonal carcinoma cells with forced expression of EWS-FLI1

2008 ◽  
Vol 90 (2) ◽  
pp. 141-150 ◽  
Author(s):  
Yu Yang ◽  
Lanjing Zhang ◽  
Yanyu Wei ◽  
Hua Wang ◽  
Mariko Fukuma ◽  
...  
Keyword(s):  
Neural Differentiation ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells

scholarly journals Substratum-growth factor collaborations are required for the mitogenic activities of activin and FGF on embryonal carcinoma cells.

1991 ◽  
Vol 114 (4) ◽  
pp. 841-846 ◽  
Author(s):  
D Schubert ◽  
H Kimura
Keyword(s):  
Tissue Culture ◽  
Embryonal Carcinoma ◽  
Activin A ◽  
Carcinoma Cells ◽  
Matrix Proteins ◽  
P19 Cells ◽  
Embryonal Carcinoma Cells ◽  
Tissue Culture Plastic ◽  
Mitogenic Response ◽  
Serum Free

When P19 mouse embryonal carcinoma cells are grown in a serum-free N2 medium on surfaces of tissue culture plastic, they die within two days. The death of these P19 cells is prevented by activin A and basic FGF (bFGF). The cells do not divide under these conditions. However, when P19 cells are cultured on substrata of extracellular matrix proteins such as laminin and fibronectin, activin A and bFGF are potent mitogens. These data show that the substratum to which cells are exposed can regulate their mitogenic response to growth factors.

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