Neural differentiation arrest in embryonal carcinoma cells with forced expression of EWS-FLI1

2008 ◽  
Vol 90 (2) ◽  
pp. 141-150 ◽  
Author(s):  
Yu Yang ◽  
Lanjing Zhang ◽  
Yanyu Wei ◽  
Hua Wang ◽  
Mariko Fukuma ◽  
...  
Keyword(s):  
Neural Differentiation ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells

scholarly journals Curcumin Induces Neural Differentiation of Human Pluripotent Embryonal Carcinoma Cells through the Activation of Autophagy

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Nudjanad Heebkaew ◽  
Narawadee Rujanapun ◽  
Phongsakorn Kunhorm ◽  
Thiranut Jaroonwitchawan ◽  
Nipha Chaicharoenaudomrung ◽  
...  
Keyword(s):  
Neural Differentiation ◽  
Embryonal Carcinoma ◽  
Curcuma Longa ◽  
Neuronal Morphology ◽  
Carcinoma Cells ◽  
Ros Generation ◽  
Dependent Manner ◽  
Embryonal Carcinoma Cells ◽  
Positive Role ◽  
Neural Lineage

Curcumin is a natural polyphenolic compound, isolated from Curcuma longa, and is an important ingredient of Asian foods. Curcumin has revealed its strong activities of anti-inflammatory, antioxidant, and anticancer. The efficient amount of curcumin could induce differentiation of stem cells and promoted the differentiation of glioma-initiating cells; however, the mechanisms underlying neural induction of curcumin have not yet been revealed. In this study, neural-inducing ability of curcumin was explored by using human pluripotent embryonal carcinoma cells, NTERA2 cells. The cells were induced toward neural lineage with curcumin and were compared with a standard neutralizing agent (retinoic acid). It was found that, after 14 days of the induction by curcumin, NTERA2 cells showed neuronal morphology and expressed neural-specific genes, including NeuroD, TUJ1, and PAX6. Importantly, curcumin activated neurogenesis of NTERA2 cells via the activation of autophagy, since autophagy-related genes, such as LC3, LAMP1, and ATG5, were upregulated along with the expression of neural genes. The inhibition of autophagy by chloroquine suppressed both autophagy and neural differentiation, highlighting the positive role of autophagy during neural differentiation. This autophagy-mediated neural differentiation of curcumin was found to be an ROS-dependent manner; curcumin induced ROS generation and suppressed antioxidant gene expression. Altogether, this study proposed the neural-inducing activity of curcumin via the regulation of autophagy within NTERA2 cells and underscored the health beneficial effects of curcumin for neurodegenerative disorders, such as Alzheimer’s disease and Parkinson’s disease.

scholarly journals Genome-wide demethylation during neural differentiation of P19 embryonal carcinoma cells

2008 ◽  
Vol 53 (2) ◽  
pp. 185-191 ◽  
Author(s):  
Izuho Hatada ◽  
Sumiyo Morita ◽  
Mika Kimura ◽  
Takuro Horii ◽  
Riu Yamashita ◽  
...  
Keyword(s):  
Neural Differentiation ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Genome Wide ◽  
P19 Embryonal Carcinoma Cells

scholarly journals Activation of c-Jun amino-terminal kinase is required for retinoic acid-induced neural differentiation of P19 embryonal carcinoma cells

FEBS Letters ◽  
2001 ◽  
Vol 503 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Hong Wang ◽  
Shoko Ikeda ◽  
Shin-ichiro Kanno ◽  
Li Ming Guang ◽  
Motoko Ohnishi ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Neural Differentiation ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Amino Terminal ◽  
P19 Embryonal Carcinoma Cells

Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation

2015 ◽  
Vol 43 (1) ◽  
pp. 9-18 ◽  
Author(s):  
Jovana Jasnic-Savovic ◽  
Andrijana Klajn ◽  
Milena Milivojevic ◽  
Marija Mojsin ◽  
Gordana Nikcevic
Keyword(s):  
Neural Differentiation ◽  
In Vitro Model ◽  
Embryonal Carcinoma ◽  
Model System ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Serum Free ◽  
Vitro Model ◽  
Serum Free Conditions

scholarly journals Okadaic Acid Suppresses Neural Differentiation-dependent Expression of the Neurofilament-L Gene in P19 Embryonal Carcinoma Cells by Post-transcriptional Modification

1996 ◽  
Vol 271 (42) ◽  
pp. 25950-25957 ◽  
Author(s):  
Yoji Sasahara ◽  
Takayasu Kobayashi ◽  
Hiroshi Onodera ◽  
Masatoshi Onoda ◽  
Motoko Ohnishi ◽  
...  
Keyword(s):  
Okadaic Acid ◽  
Neural Differentiation ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
P19 Embryonal Carcinoma Cells
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