Effects of Membrane Directed Neurotoxicants on the Contractile Activity of Cultured Skeletal Muscle Cells

1990 ◽  
Vol 17 (3) ◽  
pp. 215-217
Author(s):  
Michael Gulden ◽  
Cordula Burghoff
Keyword(s):  
Skeletal Muscle ◽  
Electrical Properties ◽  
Contractile Activity ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Rat Skeletal Muscle ◽  
Excitable Membranes ◽  
Anemonia Sulcata ◽  
Quantifying In

The effects of the sodium channel toxins saxitoxin, veratridine, deltamethrin and Anemonia sulcata toxin II on the spontaneous contractile activity of cultured rat skeletal muscle cells, were investigated. In addition, the influence of the external concentrations of K+ and Ca2+ ions was studied. The results indicate that the spontaneous contractility of cultured muscle cells is a suitable endpoint for assessing subtle alterations in the electrical properties of excitable membranes, and for quantifying in vitro the potency of neurotoxicants acting on the excitable membranes of nerve and muscle cells.

Characterization of β-adrenoceptors on rat skeletal muscle cells grown in vitro

1990 ◽  
Vol 40 (5) ◽  
pp. 1043-1048 ◽  
Author(s):  
Marie-Helene Disatnik ◽  
Sanford R. Sampson ◽  
Asher Shainberg
Keyword(s):  
Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Rat Skeletal Muscle

scholarly journals In vitro anti-diabetic assessment of guavanoic acid functionalized gold nanoparticles in regulating glucose transport using L6 rat skeletal muscle cells

2020 ◽  
Vol 11 (7) ◽  
pp. 814-822
Author(s):  
K. Govindaraju ◽  
K. S. Uma Suganya
Keyword(s):  
Skeletal Muscle ◽  
Gold Nanoparticles ◽  
Glucose Uptake ◽  
Glucose Transport ◽  
Muscle Cells ◽  
Pi3k Inhibitor ◽  
Skeletal Muscle Cells ◽  
Rat Skeletal Muscle ◽  
Functionalized Gold Nanoparticles

Glucose uptake patterns of guavanoic acid and guavanoic acid functionalized gold nanoparticles in the presence of genistein (IRTK inhibitor) and wortmannin (PI3K inhibitor).

Effects of the First Twenty MEIC Reference Chemicals on Viability, Glucose Consumption and Spontaneous Contractility of Primary Cultured Rat Skeletal Muscle Cells

1992 ◽  
Vol 20 (2) ◽  
pp. 222-225
Author(s):  
Michael Gulden ◽  
Jutta Finger
Keyword(s):  
Skeletal Muscle ◽  
Biological Activities ◽  
Glucose Consumption ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Cytotoxic Action ◽  
Rat Skeletal Muscle ◽  
Site Specific ◽  
Excitable Membranes ◽  
A Site

Primary cultured rat skeletal muscle cells were used to determine concentration-dependent effects of the first twenty MEIC chemicals on three endpoints, spontaneous contractility and viability after 1 and 24 hours, and glucose consumption during 24 hours of exposure. The contractions of cultured muscle cells depend on spontaneous electrical activity of the excitable cell membranes. The majority of the test compounds inhibited contractility at concentrations which affected neither viability nor glucose consumption. Most of these compounds are known to interact with excitable membranes in a site-specific or non-site-specific manner, thereby causing therapeutically intended or toxic effects. The results indicate that inhibition of spontaneous contractility of cultured skeletal muscle cells may reflect important non-cytotoxic biological activities of test chemicals which might be more relevant for their acute toxicity than cytotoxic action.

scholarly journals AN IN VITRO STUDY OF SYZYGIUM CUMINI SEED EXTRACT ON GLUCOSE UPTAKE ACTIVITY IN L-6 CELL LINES

2019 ◽  
Vol 9 (4-A) ◽  
pp. 256-259
Author(s):  
Maneemegalai Sivaprakasam ◽  
Narmatha M
Keyword(s):  
Diabetes Mellitus ◽  
Skeletal Muscle ◽  
Glucose Uptake ◽  
Ethanol Extract ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Syzygium Cumini ◽  
Rat Skeletal Muscle ◽  
Uptake Activity

Diabetes mellitus is the most common endocrine disorder. The plant Syzygium cumini has been used in traditional medicine for the treatment of diabetes. The present study investigated the effect of ethanol extract of S. cumini seeds on uptake of glucose by L-6 rat skeletal muscle cells. S. cumini seeds were extracted with varying solvents and quantitative phytochemical analysis was carried out, ethanol extract of seeds exhibited higher content of tested phytochemicals. The effect of different concentrations (300µg/ml – 1000µg/ml) of ethanol extract of seeds were studied on glucose uptake activity of L-6 rat skeletal muscle cells. It was observed that with the increase in concentration, the glucose uptake activity was also increased. The results of the study supports and demonstrates the antidiabetic potential of ethanol seed extracts of Syzygium cumini utilizing in vitro model. KEY WORDS: Diabetes mellitus, Syzygium cumini, phytochemicals, glucose uptake, L-6 cells

Characterization of contraction-inducible CXC chemokines and their roles in C2C12 myocytes

2009 ◽  
Vol 297 (4) ◽  
pp. E866-E878 ◽  
Author(s):  
Taku Nedachi ◽  
Hiroyasu Hatakeyama ◽  
Tatsuyoshi Kono ◽  
Masaaki Sato ◽  
Makoto Kanzaki
Keyword(s):  
Skeletal Muscle ◽  
Skeletal Muscles ◽  
Contractile Activity ◽  
Electric Pulse ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Negative Feedback Regulation ◽  
Chemokine Production

Physical exercise triggers the release of several cytokines/chemokines from working skeletal muscles, but the underlying mechanism(s) by which skeletal muscles decipher and respond to highly complex contractile stimuli remains largely unknown. In an effort to investigate the regulatory mechanisms of the expressions of two contraction-inducible CXC chemokines, CXCL1/KC and CXCL5/LIX, in contracting skeletal muscle cells, we took advantage of our in vitro exercise model using highly developed contractile C2C12 myotubes, which acquire properties similar to those of in vivo skeletal muscle via manipulation of Ca2+ transients with electric pulse stimulation (EPS). Production of these CXC chemokines was immediately augmented by EPS-evoked contractile activity in a manner dependent on the activities of JNK and NF-κB, but not p38, ERK1/2, or calcineurin. Intriguingly, exposure of myotubes to cyclic mechanical stretch also induced expression of these CXC chemokines; however, a much longer period of stimulation (∼12 h) was required, despite rapid JNK phosphorylation. We also demonstrate herein that CXCL1/KC and CXCL5/LIX have the ability to raise intracellular Ca2+ concentrations via CXCR2-mediated activation of pertussis toxin-sensitive Gαi proteins in C2C12 myoblasts, an action at least partially responsible for their migration and differentiation. Although we revealed a possible negative feedback regulation of their own production in response to the contractile activity in differentiated myotubes, exogenous administration of these CXC chemokines did not acutely influence either insulin-induced Akt phosphorylation or GLUT4 translocation in C2C12 myotubes. Taken together, these data shed light on the fundamental characteristics of contraction-inducible CXC chemokine production and their potential roles in skeletal muscle cells.

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