Determination of reduction in leaf photosynthesis due to drought conditions is complicated by plant-to-plant variability in the progressive onset of drought stress. In this study a method of analysis was developed to quantitatively compare the reduction in leaf photosynthesis due to drought stress for an old and a new maize (Zea mays L.) hybrid introduced 30 yr apart in Ontario.Key words: Maize, leaf photosynthesis, drought stress, non-linear fitting, hybrid difference