scholarly journals A New Bioartificial Liver Using Porcine Hepatocyte Spheroids in High-Cell-Density Suspension Perfusion Culture: In Vitro Performance in Synthesized Culture Medium and in 100% Human Plasma

1999 ◽  
Vol 8 (5) ◽  
pp. 531-541 ◽  
Author(s):  
Yasuyuki Sakai ◽  
Katsutoshi Naruse ◽  
Ikuo Nagashima ◽  
Tetsuichiro Muto ◽  
Motoyuki Suzuki
Keyword(s):  
Human Plasma ◽  
Culture Medium ◽  
High Cell Density ◽  
Perfusion Culture ◽  
Bioartificial Liver ◽  
High Cell ◽  
Porcine Hepatocyte ◽  
Hepatocyte Spheroids ◽  
In Vitro Performance

Comparisons of Porcine Hepatocyte Spheroids and Single Hepatocytes in the Non-Woven Fabric Bioartificial Liver Module

1996 ◽  
Vol 19 (10) ◽  
pp. 605-609 ◽  
Author(s):  
K. Naruse ◽  
Y. Sakai ◽  
I. Nagashima ◽  
G.X. Jiang ◽  
M. Suzuki ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Bioartificial Liver ◽  
Woven Fabric ◽  
Culture Vessel ◽  
Lower Efficiency ◽  
Pig Liver ◽  
Cell Basis ◽  
Porcine Hepatocyte ◽  
Hepatocyte Spheroids

We previously developed a new bioreactor of the bioartificial liver composed of non-woven fabric. We have also experimented with hepatocyte spheroids, with the aim of improving the efficiency of this NWF bioreactor. In this study, we compared the efficiencies of NWF bioreactors employing hepatocyte spheroids versus single hepatocytes. Hepatocytes were isolated from a whole pig liver by Seglen's method. 1.0 × 1010 single hepatocytes were immobilized in the NWF bioreactor. Another 1.0 × 1010 hepatocytes were allowed to form spheroids by 24 hr suspension culture in a 4-L culture vessel, before being immobilized in the bioreactor. Hepatocyte spheroids were found to be functionally superior, on a per-cell basis, to single hepatocytes in the NWF bioreactor. However, the NWF bioreactor employing hepatocyte spheroids exhibited lower efficiency than that employing single hepatocytes, because the total number of the hepatocytes had decreased during the 24 hr suspension culture.

scholarly journals High Cell Density Perfusion Culture has a Maintained Exoproteome and Metabolome

2018 ◽  
Vol 13 (10) ◽  
pp. 1800036 ◽  
Author(s):  
Leila Zamani ◽  
Magnus Lundqvist ◽  
Ye Zhang ◽  
Magnus Aberg ◽  
Fredrik Edfors ◽  
...  
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
Perfusion Culture ◽  
High Cell

scholarly journals Cold storage of porcine hepatocyte spheroids for spheroid bioartificial liver

2019 ◽  
Vol 26 (4) ◽  
Author(s):  
Yi Li ◽  
Harvey S. Chen ◽  
Mohammed Shaheen ◽  
Dong Jin Joo ◽  
Bruce P. Amiot ◽  
...  
Keyword(s):  
Cold Storage ◽  
Bioartificial Liver ◽  
Porcine Hepatocyte ◽  
Hepatocyte Spheroids

Contribution of bone marrow mesenchymal stem cells to porcine hepatocyte culture in vitro

2009 ◽  
Vol 87 (4) ◽  
pp. 595-604 ◽  
Author(s):  
Jinyang Gu ◽  
Xiaolei Shi ◽  
Xuehui Chu ◽  
Yue Zhang ◽  
Yitao Ding
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bioartificial Liver ◽  
Urea Synthesis ◽  
Soluble Factors ◽  
Cell Functions ◽  
Marrow Mesenchymal Stem Cells ◽  
Porcine Hepatocyte

One of the greatest challenges in the attempt to create functional bioartificial liver designs is the maintenance of porcine hepatocyte differentiated functions in vitro. Co-cultivation of hepatocytes with nonparenchymal cells may be beneficial for optimizing cell functions via mimicry of physiological microenvironment. However, the underlying mechanisms remain to be elucidated. An equal number of freshly isolated porcine hepatocytes and purified bone marrow mesenchymal stem cells (MSCS) was randomly co-cultured and the morphological and functional changes of heterotypic interactions were characterized. Furthermore, contributions of soluble factors involved in the separated co-culture system were evaluated. The purity of the third-passage MSCS and primary hepatocytes was more than 90% and 99%, respectively. Hepatocyte viability was greater than 95%. A rapid attachment and self-organization of three-dimensional hepatocyte spheroids were encouraged, which was due to the supporting MSCS of high motility. The elevated induction of both albumin production and urea synthesis was achieved in co-culture (P < 0.05). Data from semipermeable membrane cultures suggested that interleukin-6 is one of the key stimulators in hepatic functional enhancement. These results demonstrate for the first time that soluble factors have beneficial effects on the preservation of hepatic morphology and functionality in the co-culture of hepatocytes with MSCS in vitro, which could represent a promising tool for tissue engineering, cell biology, and bioartificial liver devices.

scholarly journals TECHNIQUE OF CULTIVATING HUMAN TISSUES IN VITRO

1916 ◽  
Vol 24 (4) ◽  
pp. 367-372 ◽  
Author(s):  
Robert A. Lambert
Keyword(s):  
Human Serum ◽  
Human Plasma ◽  
Culture Medium ◽  
Mechanical Injury ◽  
Human Tissues ◽  
Tissue Cultures ◽  
Active Growth ◽  
Homologous Serum

1. Unmodified human plasma is not a satisfactory culture medium for human tissues owing to the susceptibility of human fibrin to digestion by tissue ferments. The necessary framework is thus destroyed before the cells begin to migrate. The difficulty can be overcome by adding to human plasma or serum a small quantity of fowl or pigeon plasma, the fibrin of which is highly resistant to digestion. Human tissues have been propagated in this medium for several months through subcultures, and growth in vitro can probably be maintained indefinitely. 2. Human tissues show no greater sensitiveness to changes in temperature and mechanical injury associated with preparation of cultures than those of lower animals. They may be preserved in an ordinary ice box at 10–15°C. as long as 6 or 8 days. Tissues obtained at operation give best results, but pieces of organs removed at autopsy 1 to 4 hours after death sometimes show active growth. 3. The presence of normally existing iso-antibodies (agglutinins and hemolysins) in human serum is without influence on the growth of human tissues in vitro. In other words, autogenous serum has no advantage in tissue cultures over homologous serum.

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