Inhibition of antibody production at high cell density following mitogen stimulation and isotype switching in vitro

AbstractCellular models are needed to study human development and disease in vitro, and to screen drugs for toxicity and efficacy. Current approaches are limited in the engineering of functional tissue models with requisite cell densities and heterogeneity to appropriately model cell and tissue behaviors. Here, we develop a bioprinting approach to transfer spheroids into self-healing support hydrogels at high resolution, which enables their patterning and fusion into high-cell density microtissues of prescribed spatial organization. As an example application, we bioprint induced pluripotent stem cell-derived cardiac microtissue models with spatially controlled cardiomyocyte and fibroblast cell ratios to replicate the structural and functional features of scarred cardiac tissue that arise following myocardial infarction, including reduced contractility and irregular electrical activity. The bioprinted in vitro model is combined with functional readouts to probe how various pro-regenerative microRNA treatment regimes influence tissue regeneration and recovery of function as a result of cardiomyocyte proliferation. This method is useful for a range of biomedical applications, including the development of precision models to mimic diseases and the screening of drugs, particularly where high cell densities and heterogeneity are important.

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Expression of putative pathogenicity-related genes inXylella fastidiosagrown at low and high cell density conditions in vitro

FEMS Microbiology Letters ◽

10.1016/s0378-1097(03)00251-9 ◽

2003 ◽

Vol 222 (1) ◽

pp. 83-92 ◽

Cited By ~ 38

Author(s):

Leandra M. Scarpari ◽

Marcio R. Lambais ◽

Denise S. Silva ◽

Dirce M. Carraro ◽

Helaine Carrer

Keyword(s):

Cell Density ◽

High Cell Density ◽

High Cell

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3D bioprinting of high cell-density heterogeneous tissue models through spheroid fusion within self-healing hydrogels

10.1101/2020.05.21.103127 ◽

2020 ◽

Author(s):

Andrew C. Daly ◽

Matthew D. Davidson ◽

Jason A. Burdick

Keyword(s):

Cell Density ◽

Spatial Organization ◽

High Cell Density ◽

Self Healing ◽

High Cell ◽

Cellular Models ◽

Functional Features ◽

Cell Densities ◽

Tissue Models

AbstractCellular models are needed to study human development and disease in vitro, including the screening of drugs for toxicity and efficacy. However, current approaches are limited in the engineering of functional tissue models with requisite cell densities and heterogeneity to appropriately model cell and tissue behaviors. Here, we develop a new bioprinting approach to transfer spheroids into self-healing support hydrogels at high resolution, which enables their patterning and fusion into high-cell density microtissues of prescribed spatial organization. As an example application, we bioprint induced pluripotent stem cell-derived cardiac microtissue models with spatially controlled cardiomyocyte and fibroblast cell ratios to replicate the structural and functional features of scarred cardiac tissue that arise following myocardial infarction, including reduced contractility and irregular electrical activity. The bioprinted in vitro model is combined with functional readouts to probe how various pro-regenerative microRNA treatment regimes influence tissue regeneration and recovery of function as a result of cardiomyocyte proliferation. This method is useful for a range of biomedical applications, including the development of precision models to mimic diseases and for the screening of drugs, particularly where high cell densities and heterogeneity are important.

Download Full-text