scholarly journals Inter-laboratory comparison of 2 ELISA kits used for foot-and-mouth disease virus nonstructural protein serology

2020 ◽  
Vol 32 (6) ◽  
pp. 933-937
Author(s):  
Clare F. J. Browning ◽  
Antonello Di Nardo ◽  
Lissie Henry ◽  
Tim Pollard ◽  
Lynne Hendry ◽  
...  
Keyword(s):  
Disease Virus ◽  
Disease Surveillance ◽  
Nonstructural Protein ◽  
Foot And Mouth Disease ◽  
Screen Test ◽  
Mouth Disease ◽  
Nonstructural Proteins ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Thermo Fisher Scientific

Serologic assays used to detect antibodies to nonstructural proteins (NSPs) of foot-and-mouth disease virus (FMDV) are used for disease surveillance in endemic countries, and are essential to providing evidence for freedom of the disease with or without vaccination and to recover the free status of a country after an outbreak. In a 5-site inter-laboratory study, we compared the performance of 2 commercial NSP ELISA kits (ID Screen FMD NSP ELISA single day [short] and overnight protocols, ID.Vet; PrioCHECK FMDV NS antibody ELISA, Thermo Fisher Scientific). The overall concordance between the PrioCHECK and ID Screen test was 93.8% (95% CI: 92.0–95.2%) and 94.8% (95% CI: 93.1–96.1%) for the overnight and short ID Screen incubation protocols, respectively. Our results indicate that the assays (including the 2 different formats of the ID Screen test) can be used interchangeably in post-outbreak serosurveillance.

scholarly journals Bovine Serum Panel for Evaluating Foot-and-Mouth Disease Virus Nonstructural Protein Antibody Tests

2007 ◽  
Vol 19 (5) ◽  
pp. 539-544 ◽  
Author(s):  
Satya Parida ◽  
Lucy Fleming ◽  
Debi Gibson ◽  
Pip A. Hamblin ◽  
Santina Grazioli ◽  
...  
Keyword(s):  
Disease Virus ◽  
Nonstructural Protein ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Infected Cattle ◽  
Nonstructural Proteins ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Contact Exposure ◽  
Antibody Tests

A panel of 36 sera has been assembled from experimental cattle that had been infected by inoculation or contact exposure with 4 serotypes of foot-and-mouth disease virus (FMDV) with or without prior vaccination. Virus replication and persistence had been characterized in all of the animals. The proportion of the sera scored positive by 5 tests for antibodies to the nonstructural proteins of FMDV varied, suggesting that the panel can discriminate between the sensitivity with which such tests are able to identify infected cattle. Use of this panel will help in assessment of new tests and quality control of existing methods.

scholarly journals Foot‐and‐mouth disease virus nonstructural protein 2B interacts with cyclophilin A, modulating virus replication

2018 ◽  
Vol 32 (12) ◽  
pp. 6706-6723 ◽  
Author(s):  
Huisheng Liu ◽  
Qiao Xue ◽  
Weijun Cao ◽  
Fan Yang ◽  
Linna Ma ◽  
...  
Keyword(s):  
Disease Virus ◽  
Virus Replication ◽  
Nonstructural Protein ◽  
Foot And Mouth Disease ◽  
Cyclophilin A ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Delivery of a foot-and-mouth disease virus empty capsid subunit antigen with nonstructural protein 2B improves protection of swine

Vaccine ◽  
2008 ◽  
Vol 26 (45) ◽  
pp. 5689-5699 ◽  
Author(s):  
Lindomar Pena ◽  
Mauro Pires Moraes ◽  
Marla Koster ◽  
Thomas Burrage ◽  
Juan M. Pacheco ◽  
...  
Keyword(s):  
Disease Virus ◽  
Nonstructural Protein ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Empty Capsid ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Diagnostic potential of recombinant nonstructural protein 3B to detect antibodies induced by foot-and-mouth disease virus infection in bovines

2014 ◽  
Vol 159 (9) ◽  
pp. 2359-2369 ◽  
Author(s):  
Amiya Kumar Mohapatra ◽  
Jajati Keshari Mohapatra ◽  
Laxmi Kant Pandey ◽  
Aniket Sanyal ◽  
Bramhadev Pattnaik
Keyword(s):  
Virus Infection ◽  
Disease Virus ◽  
Nonstructural Protein ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Diagnostic Potential ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Foot-and-Mouth Disease Virus Nonstructural Protein 2C Interacts with Beclin1, Modulating Virus Replication

2012 ◽  
Vol 86 (22) ◽  
pp. 12080-12090 ◽  
Author(s):  
D. P. Gladue ◽  
V. O'Donnell ◽  
R. Baker-Branstetter ◽  
L. G. Holinka ◽  
J. M. Pacheco ◽  
...  
Keyword(s):  
Disease Virus ◽  
Virus Replication ◽  
Nonstructural Protein ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Mutagenesis Mapping of RNA Structures within the Foot-and-Mouth Disease Virus Genome Reveals Functional Elements Localized in the Polymerase (3D pol )-Encoding Region

mSphere ◽  
2021 ◽  
Author(s):  
Lidia Lasecka-Dykes ◽  
Fiona Tulloch ◽  
Peter Simmonds ◽  
Garry A. Luke ◽  
Paolo Ribeca ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Virus Genome ◽  
Mouth Disease ◽  
Rna Structures ◽  
Nonstructural Proteins ◽  
Functional Elements ◽  
Subgenomic Replicon ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Some RNA structures formed by the genomes of RNA viruses are critical for viral replication. Our study shows that of 46 conserved RNA structures located within the regions of the foot-and-mouth disease virus (FMDV) genome that encode the nonstructural proteins, only 3 are essential for replication of an FMDV subgenomic replicon.

scholarly journals Identification of T-Cell Epitopes in Nonstructural Proteins of Foot-and-Mouth Disease Virus

2001 ◽  
Vol 75 (7) ◽  
pp. 3164-3174 ◽  
Author(s):  
E. Blanco ◽  
M. Garcia-Briones ◽  
A. Sanz-Parra ◽  
P. Gomes ◽  
E. De Oliveira ◽  
...  
Keyword(s):  
T Cell ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
T Cell Epitopes ◽  
Nonstructural Proteins ◽  
Mouth Disease Virus ◽  
Foot And Mouth
Sign in / Sign up

Export Citation Format

Share Document