scholarly journals A Versatile High Throughput Screen for Dioxygenase Activity Using Solid-Phase Digital Imaging

2001 ◽  
Vol 6 (4) ◽  
pp. 219-223 ◽  
Author(s):  
John M. Joern ◽  
Takeshi Sakamoto ◽  
Akira Arisawa ◽  
Frances H. Arnold
Keyword(s):  
Image Analysis ◽  
Directed Evolution ◽  
High Throughput ◽  
Digital Imaging ◽  
Solid Phase ◽  
High Throughput Screen ◽  
Microplate Reader ◽  
Dihydrodiol Dehydrogenase ◽  
Dioxygenase Activity

We have developed a solid-phase, high throughput (10,000 clones/day) screen for dioxygenase activity. The cis-di- hydrodiol product of dioxygenase bioconversion is converted to a phenol by acidification or to a catechol by reaction with cis-dihydrodiol dehydrogenase. Gibbs reagent reacts quickly with these oxygenated aromatics to yield colored products that are quantifiable using a microplate reader or by digital imaging and image analysis. The method is reproducible and quantitative at product concentrations of only 30,uM, with essentially no background from media components. This method is an effective general screen for aromatic oxidation and should be a useful tool for the discovery and directed evolution of oxygenases.

scholarly journals A high-throughput digital imaging screen for the discovery and directed evolution of oxygenases

1999 ◽  
Vol 6 (10) ◽  
pp. 699-706 ◽  
Author(s):  
Hyun Joo ◽  
Akira Arisawa ◽  
Zhanglin Lin ◽  
Frances H Arnold
Keyword(s):  
Directed Evolution ◽  
High Throughput ◽  
Digital Imaging

scholarly journals Automated screening ofC. elegansneurodegeneration mutants enabled by microfluidics and image analysis algorithms

2018 ◽  
Vol 10 (9) ◽  
pp. 539-548 ◽  
Author(s):  
Ivan de Carlos Cáceres ◽  
Daniel A. Porto ◽  
Ivan Gallotta ◽  
Pamela Santonicola ◽  
Josue Rodríguez-Cordero ◽  
...  
Keyword(s):  
Image Analysis ◽  
Spinal Muscular Atrophy ◽  
High Throughput ◽  
Muscular Atrophy ◽  
High Throughput Screen ◽  
C Elegans ◽  
Genetic Mechanisms ◽  
Automated Screening

A fully automated high-throughput screen usingC. elegansto investigate genetic mechanisms affecting spinal muscular atrophy (SMA).

scholarly journals Ultra-high throughput functional enrichment of large monoamine oxidase (MAO-N) libraries by fluorescence activated cell sorting

The Analyst ◽  
2018 ◽  
Vol 143 (19) ◽  
pp. 4747-4755 ◽  
Author(s):  
Joanna C. Sadler ◽  
Andrew Currin ◽  
Douglas B. Kell
Keyword(s):  
Monoamine Oxidase ◽  
Directed Evolution ◽  
High Throughput ◽  
Cell Sorting ◽  
Oxidase Activity ◽  
Functional Enrichment ◽  
High Throughput Screen ◽  
Fluorescence Activated Cell Sorting ◽  
E Coli

A novel ultra-high throughput screen forin vivodetection of oxidase activity inE. colicells and its application to directed evolution.

Combinatorial Mutagenesis Algorithms, Digital Imaging Spectroscopy, and Solid-Phase Assays for Directed Evolution

2003 ◽  
Author(s):  
Simon Delagrave ◽  
Edward Bylina ◽  
William Coleman ◽  
Steven Robles ◽  
Mary Yang ◽  
...  
Keyword(s):  
Directed Evolution ◽  
Digital Imaging ◽  
Solid Phase ◽  
Imaging Spectroscopy ◽  
Combinatorial Mutagenesis

Development of a High-Throughput Screen for Protein Catalysts: Application to the Directed Evolution of Antibody Aldolases

2003 ◽  
Vol 115 (48) ◽  
pp. 6153-6155
Author(s):  
Jeff Gildersleeve ◽  
Alex Varvak ◽  
Shane Atwell ◽  
Doug Evans ◽  
Peter G. Schultz
Keyword(s):  
Directed Evolution ◽  
High Throughput ◽  
High Throughput Screen

A High-Throughput Screen for Porphyrin Metal Chelatases: Application to the Directed Evolution of Ferrochelatases for Metalloporphyrin Biosynthesis

ChemBioChem ◽  
2004 ◽  
Vol 5 (8) ◽  
pp. 1069-1074 ◽  
Author(s):  
Seok Joon Kwon ◽  
Ralf Petri ◽  
Arjo L. deBoer ◽  
Claudia Schmidt-Dannert
Keyword(s):  
Directed Evolution ◽  
High Throughput ◽  
High Throughput Screen

scholarly journals High‐Throughput Solid‐Phase Assay for Substrate Profiling and Directed Evolution of Transketolase

ChemBioChem ◽  
2021 ◽  
Author(s):  
Nazim Ocal ◽  
Aurélie Lagarde ◽  
Mélanie L'enfant ◽  
Franck Charmantray ◽  
Laurence Hecquet
Keyword(s):  
Directed Evolution ◽  
High Throughput ◽  
Solid Phase ◽  
Solid Phase Assay

Development of a High-Throughput Screen for Protein Catalysts: Application to the Directed Evolution of Antibody Aldolases

2003 ◽  
Vol 42 (48) ◽  
pp. 5971-5973 ◽  
Author(s):  
Jeff Gildersleeve ◽  
Alex Varvak ◽  
Shane Atwell ◽  
Doug Evans ◽  
Peter G. Schultz
Keyword(s):  
Directed Evolution ◽  
High Throughput ◽  
High Throughput Screen

Antisense RNA-based High-Throughput Screen System for Directed Evolution of Quorum Quenching Enzymes

2015 ◽  
Vol 10 (11) ◽  
pp. 2598-2605 ◽  
Author(s):  
Sang-Soo Han ◽  
Won-Ji Park ◽  
Hak-Sung Kim ◽  
Geun-Joong Kim
Keyword(s):  
Directed Evolution ◽  
High Throughput ◽  
Antisense Rna ◽  
Quorum Quenching ◽  
High Throughput Screen ◽  
Screen System

scholarly journals Glycine Oxidase Based High-Throughput Solid-Phase Assay for Substrate Profiling and Directed Evolution of (R)- and (S)-Selective Amine Transaminases

2014 ◽  
Vol 86 (23) ◽  
pp. 11847-11853 ◽  
Author(s):  
Martin S. Weiß ◽  
Ioannis V. Pavlidis ◽  
Clare Vickers ◽  
Matthias Höhne ◽  
Uwe T. Bornscheuer
Keyword(s):  
Directed Evolution ◽  
High Throughput ◽  
Solid Phase ◽  
Solid Phase Assay ◽  
Glycine Oxidase
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