antisense rna
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Bioengineered ◽  
2022 ◽  
Vol 13 (2) ◽  
pp. 2296-2307
Yabo Liu ◽  
Huibo Li ◽  
Yanqiu Zhao ◽  
Dandan Li ◽  
Qian Zhang ◽  

mBio ◽  
2022 ◽  
Taylor Van Gundy ◽  
Edward Martin ◽  
Jeremy Bono ◽  
Olivia Hatton ◽  
Meghan C. Lybecker

Next-generation RNA sequencing of numerous organisms has revealed that transcription is widespread across the genome, termed pervasive transcription, and does not adhere to annotated gene boundaries. The function of pervasive transcription is enigmatic and has generated considerable controversy as to whether it is transcriptional noise or biologically relevant.

2022 ◽  
Vol 23 (1) ◽  
pp. 576
Laurène Bastet ◽  
Pilar Bustos-Sanmamed ◽  
Arancha Catalan-Moreno ◽  
Carlos J. Caballero ◽  
Sergio Cuesta ◽  

Bacterial genomes are pervasively transcribed, generating a wide variety of antisense RNAs (asRNAs). Many of them originate from transcriptional read-through events (TREs) during the transcription termination process. Previous transcriptome analyses revealed that the lexA gene from Staphylococcus aureus, which encodes the main SOS response regulator, is affected by the presence of an asRNA. Here, we show that the lexA antisense RNA (lexA-asRNA) is generated by a TRE on the intrinsic terminator (TTsbrB) of the sbrB gene, which is located downstream of lexA, in the opposite strand. Transcriptional read-through occurs by a natural mutation that destabilizes the TTsbrB structure and modifies the efficiency of the intrinsic terminator. Restoring the mispairing mutation in the hairpin of TTsbrB prevented lexA-asRNA transcription. The level of lexA-asRNA directly correlated with cellular stress since the expressions of sbrB and lexA-asRNA depend on the stress transcription factor SigB. Comparative analyses revealed strain-specific nucleotide polymorphisms within TTsbrB, suggesting that this TT could be prone to accumulating natural mutations. A genome-wide analysis of TREs suggested that mispairings in TT hairpins might provide wider transcriptional connections with downstream genes and, ultimately, transcriptomic variability among S. aureus strains.

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