scholarly journals Pancreatic Islet Hyperplasia

2018 ◽  
Vol 8 (3) ◽  
pp. 777-785
Author(s):  
Jared Weinand ◽  
Walter L. Kemp
Keyword(s):  
Pancreatic Islet ◽  
Athletic Performance ◽  
Islet Cells ◽  
Islet Hyperplasia ◽  
Organ Systems ◽  
Personal Appearance ◽  
Androgenic Steroids ◽  
Recreational Use ◽  
Histologic Changes

It has been estimated that up to four million Americans have used anabolic-androgenic steroids (AAS) to increase muscle mass – either for improved athletic performance, enhanced personal appearance, or both. While the pathologic effects of supra-physiologic doses of AAS have been well-described for some organ systems, such as the cardiovascular system, the effects on other organ systems are less well-described; for example, there is a dearth of knowledge in the medical literature regarding the effects of recreational use of AAS on the islet cells of the endocrine pancreas. As pancreatic islet hyperplasia has previously been described in the literature in a group of patients receiving long-term AAS treatment for Fanconi anemia, it is reasonable to suggest that the use of AAS by bodybuilders could produce the same (or similar) histologic changes. We present a case that offers support for the association of anabolic-androgenic steroid use and pancreatic islet hyperplasia.

scholarly journals Systematic prevention of bubble formation and accumulation for long-term culture of pancreatic islet cells in microfluidic device

2012 ◽  
Vol 14 (2) ◽  
pp. 419-426 ◽  
Author(s):  
Yong Wang ◽  
Dongyoung Lee ◽  
Lisa Zhang ◽  
Hyojin Jeon ◽  
Joshua E. Mendoza-Elias ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Microfluidic Device ◽  
Islet Cells ◽  
Bubble Formation ◽  
Pancreatic Islet Cells ◽  
Long Term Culture

The Effects of Long Term Ketamine Use on Pancreatic Islet Cells

2013 ◽  
Vol 33 (4) ◽  
pp. 1088-1096 ◽  
Author(s):  
Habib BOSTAN ◽  
Yakup TOMAK ◽  
Deniz ÜNAL ◽  
Levent TÜMKAYA ◽  
Durdu ALTUNER ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells

Long-term culture of pancreatic islet cells with special reference to the β-cell function

In Vitro ◽  
1979 ◽  
Vol 15 (2) ◽  
pp. 95-102 ◽  
Author(s):  
Junko Ono ◽  
Ryosaburo Takaki ◽  
Hiromitsu Okano ◽  
Michio Fukuma
Keyword(s):  
Special Reference ◽  
Pancreatic Islet ◽  
Cell Function ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Β Cell ◽  
Long Term Culture ◽  
Β Cell Function

scholarly journals In VitroProliferation of Porcine Pancreatic Islet Cells forβ-Cell Therapy Applications

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Guoguang Niu ◽  
John P. McQuilling ◽  
Yu Zhou ◽  
Emmanuel C. Opara ◽  
Giuseppe Orlando ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Diabetic Patients ◽  
Pancreatic Progenitors ◽  
Insulin Expression ◽  
Insulin Secreting Cells ◽  
Alternative Sources ◽  
Different Sources

β-Cell replacement through transplantation is the only curative treatment to establish a long-term stable euglycemia in diabetic patients. Owing to the shortage of donor tissue, attempts are being made to develop alternative sources of insulin-secreting cells. Stem cells differentiation and reprograming as well as isolating pancreatic progenitors from different sources are some examples; however, no approach has yet yielded a clinically relevant solution. Dissociated islet cells that are cultured in cell numbers byin vitroproliferation provide a promising platform for redifferentiation towardsβ-cells phenotype. In this study, we cultured islet-derived cellsin vitroand examined the expression ofβ-cell genes during the proliferation. Islets were isolated from porcine pancreases and enzymatically digested to dissociate the component cells. The cells proliferated well in tissue culture plates and were subcultured for no more than 5 passages. Only 10% of insulin expression, as measured by PCR, was preserved in each passage. High glucose media enhanced insulin expression by about 4–18 fold, suggesting a glucose-dependent effect in the proliferated islet-derived cells. The islet-derived cells also expressed other pancreatic genes such as Pdx1, NeuroD, glucagon, and somatostatin. Taken together, these results indicate that pancreatic islet-derived cells, proliferatedin vitro, retained the expression capacity for key pancreatic genes, thus suggesting that the cells may be redifferentiated into insulin-secretingβ-like cells.

Long-Term Culture of Isolated Pancreatic Islet Cells

1975 ◽  
Vol 149 (2) ◽  
pp. 402-406 ◽  
Author(s):  
R. Takaki ◽  
J. Ono ◽  
M. Fukuma ◽  
T. Ikegami
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Long Term Culture

scholarly journals Alginate encapsulation as long-term immune protection of allogeneic pancreatic islet cells transplanted into the omental bursa of macaques

2018 ◽  
Vol 2 (11) ◽  
pp. 810-821 ◽  
Author(s):  
Matthew A. Bochenek ◽  
Omid Veiseh ◽  
Arturo J. Vegas ◽  
James J. McGarrigle ◽  
Meirigeng Qi ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Immune Protection ◽  
Pancreatic Islet Cells ◽  
Alginate Encapsulation ◽  
Omental Bursa

Silver Staining of Pancreatic Islets as Revealed by Electron Microscopy

1967 ◽  
Vol 25 ◽  
pp. 44-45
Author(s):  
Kazuaki Misugi ◽  
Nobuko Misugi ◽  
Hiroshi Yamada
Keyword(s):  
Pancreatic Islet ◽  
Silver Staining ◽  
Islet Cells ◽  
Severe Hypoglycemia ◽  
Granular Cell ◽  
Electron Microscopic Level ◽  
Staining Reaction ◽  
Electron Microscopic ◽  
Pancreatic Islet Cell ◽  
D Cell

The authors had described the fine structure of a type of pancreatic islet cell, which appeared different from typical alpha and beta cells, and tentatively considered that this third type of granular cell probably represents the D cell (Figure 1).Since silver staining has been widely used to differentiate different types of pancreatic islet cells by light microscopy, an attempt to examine this staining reaction at the electron microscopic level was made.Material and Method: Surgically removed specimens from three infants who suffered from severe hypoglycemia were used. The specimens were fixed and preserved in 20% neutral formalin. Frozen sections, 30 to 40 micron thick, were prepared and they were stained by Bielschowsky's method as modified by Suzuki (2). The stained sections were examined under a microscope and islet tissues were isolated. They were fixed in 1% osmium tetroxide in phosphate buffer for one hour and embedded in Epon 812 following dehydration through a series of alcohols and propylene oxide.

Monolayer cultures of adult pancreatic islet cells on osmotically disrupted fibroblasts

Diabetes ◽  
1980 ◽  
Vol 29 (6) ◽  
pp. 497-500 ◽  
Author(s):  
P. Meda ◽  
E. L. Hooghe-Peters ◽  
L. Orci
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Monolayer Cultures
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