scholarly journals Aluminum phosphate visualisation of acid phosphatase activity: a biochemical and x-ray microanalysis study.

1982 ◽  
Vol 30 (1) ◽  
pp. 86-90 ◽  
Author(s):  
J P Berry ◽  
J Hourdry ◽  
M Sternberg ◽  
P Galle
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Rat Kidney ◽  
Aluminum Phosphate ◽  
Proximal Tubules ◽  
New Method ◽  
X Ray ◽  
Biochemical Studies

A new method is described that demonstrates acid phosphatase activity in the cells of the proximal tubules of the rat kidney. The method is based on the formation of an insoluble aluminum phosphate precipitate. Microanalysis was used to demonstrate the presence of intracellular aluminum and determine the quantity present under the probe. Parallel biochemical studies showed that the aluminum precipitate was indeed due to acid phosphatase activity.

Physiological studies on trematodes: phosphatase systems in Gastrothylax crumenifer

Parasitology ◽  
1966 ◽  
Vol 56 (1) ◽  
pp. 105-110 ◽  
Author(s):  
Madan M. Goil
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Maximum Activity ◽  
Fluoride Ions ◽  
Gastrothylax Crumenifer ◽  
Biochemical Studies ◽  
Physiological Studies ◽  
Phosphatase Enzyme

Biochemical studies on the phosphatase systems of Gastrothylax crumenifer have been made. The maximum activity of the phosphatase enzyme was found to be at 5 pH. The action of magnesium and fluoride ions on the acid phosphatase activity shows that both act as inhibitors. The day-to-day variation in the phosphatase activity of the samples, as measured by block differences, was found to be significant at different pH levels. The heat denatured extract showed low and fairly constant acid phosphatase activity.

An X-ray microanalytical azo dye technique for the localization of acid phosphatase activity

1976 ◽  
Vol 49 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Ivor D. Bowen ◽  
Timothy A. Ryder ◽  
Nerys L. Downing
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Azo Dye ◽  
X Ray

An improved procedure for the X-ray microanalysis of acid phosphatase activity in lysosomes

1987 ◽  
Vol 87 (1) ◽  
pp. 71-77 ◽  
Author(s):  
J. B. van Dorf ◽  
J. Ph. Zeelen ◽  
W. C. de Bruijn
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
X Ray
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