Abstract
Background Canine tick-borne haemopathogens (TBH) constitute a significant concern worldwide. The detection of these TBH is mainly achieved by microscopic evaluation, seroprevalence, and molecular detection. The present study was designed to investigate the molecular detection of Anaplasma platys , Babesia gibsoni, Babesia vogeli , and Ehrlichia canis in shelter dogs and Rhipicephalus sanguineus ( sensu lato ) ticks infesting them in Malaysia.Results A total of 220 blood samples and 140 ticks were collected from 10 animal shelters in Peninsular Malaysia. The presence of haemopathogens was detected using conventional PCR, sequenced, and identified at the species level. Of the 220 blood samples, 77 (35%) were positive with at least one of the four haemopathogens, of which E. canis predominated (20%) followed by B. gibsoni and B. vogeli both with detection of 7%, and A. platys (12%). In tick samples, 5 (3.57%) of the collected samples were positive with at least one of the three haemopathogens. Low detection of E. canis and A. platys were present (n = 2; 1.43%) as well as B. vogeli shows only 1 sample positive (0.71%) and no detection of B. gibsoni . For co-infection in dogs, single infection is common (24%), while co-infection with two haemopathogens (10%) was also observed. The occurrence of infection with three TBH was also observed in the sampled dogs (1%).Conclusion E. canis is the most common TBH affecting shelter dogs in Peninsular Malaysia. Co-infection is quite common and the most common co-infection present was E. canis and A. platys. The study highlighted the first extensive molecular detection of TBH in dogs and R. sanguineus ( sensu lato ) ticks in Malaysia