Polyphosphoinositides, tyrosine kinase and sea urchin egg activation

2020 ◽  
pp. 41-50
Author(s):  
Brigitte Ciapa ◽  
Beatrice Borg ◽  
David Epel
Keyword(s):  
Tyrosine Kinase ◽  
Sea Urchin ◽  
Egg Activation ◽  
Sea Urchin Egg

Ryanodine Activates Sea Urchin Eggs. (sea urchin egg/activation/ryanodine/inositol phosphate/heparin)

1992 ◽  
Vol 34 (1) ◽  
pp. 37-42 ◽  
Author(s):  
C. Sardet ◽  
I. Gillot ◽  
A. Ruscher ◽  
P. Payan ◽  
J.-P. Girard ◽  
...  
Keyword(s):  
Sea Urchin ◽  
Inositol Phosphate ◽  
Egg Activation ◽  
Sea Urchin Eggs ◽  
Sea Urchin Egg

Characterisation and role of integrins during gametic interaction and egg activation

Zygote ◽  
1996 ◽  
Vol 4 (1) ◽  
pp. 31-40 ◽  
Author(s):  
Céline de Nadai ◽  
Patrick Fenichel ◽  
Michèle Donzeau ◽  
David Epel ◽  
Brigitte Ciapa
Keyword(s):  
Sea Urchin ◽  
Cytoskeletal Proteins ◽  
Human Spermatozoa ◽  
Egg Activation ◽  
Western Blots ◽  
Sea Urchin Egg ◽  
Immunofluorescence Labelling ◽  
Β Chain

SummaryIt has recently been proposed that some of the processes induced by fertilisation in mammals may be mediated by integrins. By peforming immunofluorescence labelling and Western blots with antibodies directed against some of the α and β subunits of integrins, we show here the presence of some of these proteins in human and hamster oocytes. Among them, α2 and α5 were also present on in vitro preparations of sea urchin egg cortices. In addition, antibodies raised against these two proteins were the most effective at inhibiting attachment and fusion of human spermatozoa with hamster oocytes. We suggest that α2 and α5 integrin chains may be common mediators in adhesion-fusion mechanisms triggered by fertilisation. Using similar techniques, we show that eggs are rich three cytoskeletal proteins known to be linked to the β chain of integrins: talin, vinculin and α-actinin. Moreover, we found that talin and α-actinin were associated with proteins phosphorylated on tyrosine after fertilisation in sea urchin eggs. We suggest that integrins might be involved during fertilisation and trigger egg activation through cytoskeletal structures.

scholarly journals 2DE identification of proteins exhibiting turnover and phosphorylation dynamics during sea urchin egg activation

2008 ◽  
Vol 313 (2) ◽  
pp. 630-647 ◽  
Author(s):  
Michelle M. Roux ◽  
Monte J. Radeke ◽  
Manisha Goel ◽  
Arcady Mushegian ◽  
Kathy R. Foltz
Keyword(s):  
Sea Urchin ◽  
Egg Activation ◽  
Sea Urchin Egg
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