Phylogenetic Analyses of Phellinus s. l. and Inonotus s. l. (Hymenochaetales) Inferred from rDNA ITS Sequences and Morphological Data

AbstractItajahya galericulata (Phallales, Phallaceae) was previously reported from several countries in South America and Africa. Recently we found I. galericulata in the city of Vadodara, Gujarat State, India. To verify its identity we studied its morphology and performed molecular phylogenetic analyses using nuclear rDNA LSU and mitochondrial ATP6 loci. Here we also provide nuclear rDNA ITS sequences for the Indian collection, since up to now no sequences of this region have been available for I. galericulata in GenBank. This study furnishes the first evidence for the occurrence of I. galericulata in India and in Asia as a whole.

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Phylogenetic relationships among Elymus and related diploid genera (Triticeae: Poaceae) based on nuclear rDNA ITS sequences

Biologia ◽

10.1515/biolog-2015-0019 ◽

2015 ◽

Vol 70 (2) ◽

Cited By ~ 6

Author(s):

Gang Gao ◽

Jia-bin Deng ◽

Xue-mei Gou ◽

Qian Wang ◽

Chun-bang Ding ◽

...

Keyword(s):

Phylogenetic Relationships ◽

Evolutionary History ◽

Phylogenetic Analyses ◽

Its Sequences ◽

Neighbor Joining ◽

Rdna Its ◽

Genome Donor ◽

Rdna Its Sequences ◽

History Of ◽

Elymus Species

AbstractTo investigate the phylogenetic relationships among Elymus and related diploid genera, the genome donor of Elymus, and the evolutionary history of polyploid Elymus species, nuclear ribosomal internal transcribed spacer (ITS) sequences were analyzed for 10 Elymus species, together with 17 diploid taxa from 5 monogenomic genera. The phylogenetic analyses (Neighbor-Joining) supported two major clades (St and H). Sequence diversity and genealogical analysis suggested that (1) Elymus species were unambiguously closely related to Pseudoroegeria; (2) Pse. stipifolia might be serve as the St genome donor of polyploid Elymus species; (3) the Y genome might be originated from ancestral lineage of Pseudoroegneria (St); (4) the ITS sequences of Elymus were evolutionarily distinct and may clarify parental lineages and phylogenetic relationships in Elymus.

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DNA From Ancient Cedar Wood From King Midas’ Tomb, Turkey, and Al-Aksa Mosque, Israel

Silvae Genetica ◽

10.1515/sg-2006-0009 ◽

2006 ◽

Vol 55 (1-6) ◽

pp. 54-62 ◽

Cited By ~ 9

Author(s):

S. O. Rogers ◽

Z. Kaya

Keyword(s):

Phylogenetic Analyses ◽

Wood Specimen ◽

Archaeological Site ◽

Internal Transcribed Spacers ◽

Its Sequences ◽

Its2 Region ◽

Its1 Region ◽

Rdna Its ◽

Lebanon Cedar ◽

Rdna Its Sequences

Abstract Ancient Taurus cedar (Cedrus libani A. Rich) wood samples from the Tumulus of King Midas at the Gordion archaeological site (about 2700 years old), near Ankara, Turkey, and from the Al-Aksa Mosque (about 1500 to 1900 years old), Jerusalem, Israel, were characterized by studying the sequences of ribosomal DNA (rDNA) internal transcribed spacers (ITS1 and ITS2). After extraction of the DNA, the ITS regions were amplified utilizing the polymerase chain reaction, followed by sequencing, BLAST searches for similar sequences, and phylogenetic analyses. Fifty-six sequences were obtained. In BLAST searches of existing sequence databases, most were closest to those from humans and fungi. However, two sequences exhibited similarities with conifer ITS sequences. One was an ITS1 region from the Gordion wood specimen, and the other one was an ITS2 region from the Al-Aksa wood specimen. Phylogenetic analyses indicated that both were closest to Taurus cedar (C. libani, also known as Lebanon cedar) ITS sequences from three recent samples of Taurus cedar from two sites in Turkey. However, they exhibited many differences from the recent C. libani rDNA ITS sequences from Turkey, probably due to degradation of the DNA in the ancient samples. The implications of the results on future studies are discussed.

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Cortinarius alpinus as an example for morphological and phylogenetic species concepts in ectomycorrhizal fungi

Sommerfeltia ◽

10.2478/v10208-011-0009-1 ◽

2008 ◽

Vol 31 (1) ◽

pp. 161-177 ◽

Cited By ~ 8

Author(s):

U. Peintner

Keyword(s):

Ectomycorrhizal Fungi ◽

Related Species ◽

Dry Weight ◽

Species Concepts ◽

Its Sequences ◽

Phylogenetic Species ◽

Closely Related Species ◽

Rdna Its ◽

Rdna Its Sequences ◽

Distinguishing Character

Cortinarius alpinus as an example for morphological and phylogenetic species concepts in ectomycorrhizal fungiExtensive morphological and molecular analyses of closely related species from alpine, subalpine and montane habitats should enable a comparison of ecological, morphological and phylogenetic species concepts in ectomycorrhizal mushrooms. One fundamental question of this study was whether alpine species really exist, and which criteria, besides the specific habitat, could reliably be used for the de-limitation of such taxa. For this reason, 56 rDNA ITS sequences were generated or downloaded from GenBank for 10 closely related species of Cortinarius subgenus Myxacium, section Myxacium. Several collections were sequenced for each of the following taxa: Cortinarius absarokensis, C. alpinus, C. favrei, C. fennoscandicus, C. grallipes, C. mucosus, C. muscigenus, C. septentrionalis, C. trivialis and C. vernicosus. Moreover, spore statistics were carried out for 38 collections of alpine and subalpine taxa. These data provide clear evidence for C. favrei being a synonym of C. alpinus. C. absarokensis and C. alpinus can clearly be delimited based on pileus diameter and average dry weight per basidiome, even in overlapping habitats, but spore size and shape is not a good distinguishing character. Phylograms have very short branches, and base differences between ITS sequences are generally very low in this group, and give no resolution for the included taxa of this section. Based on these results, species concepts of ectomycorrhizal mushrooms are discussed in detail.

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