scholarly journals Receptors Specific for the Carboxyl-Terminal Region of Parathyroid Hormone on Bone-Derived Cells: Determinants of Ligand Binding and Bioactivity

Endocrinology ◽  
2005 ◽  
Vol 146 (4) ◽  
pp. 1863-1870 ◽  
Author(s):  
P. Divieti ◽  
A. I. Geller ◽  
G. Suliman ◽  
H. Jüppner ◽  
F. R. Bringhurst
Keyword(s):  
Parathyroid Hormone ◽  
Ligand Binding ◽  
Terminal Region ◽  
Carboxyl Terminal

Parathyrin radioimmunoassay: diagnostic utility of antisera produced against carboxyl-terminal fragments of the hormone from the human.

1978 ◽  
Vol 24 (3) ◽  
pp. 451-454 ◽  
Author(s):  
F P Di Bella ◽  
J M Kehrwald ◽  
K Laakso ◽  
L Zitzner
Keyword(s):  
Renal Failure ◽  
Parathyroid Hormone ◽  
Chronic Renal Failure ◽  
Guinea Pigs ◽  
Normal Subjects ◽  
Terminal Region ◽  
Diagnostic Utility ◽  
Human Origin ◽  
Widespread Availability ◽  
Carboxyl Terminal

Abstract Antisera directed toward the carboxyl-terminal region of human parathyrin (parathyroid hormone), for use in daignostically applicable radioimmunoassays of the hormone in serum, are scarce, largely because of the lack of suitable immunogens of human origin. We produced four antisera in goats and guinea pigs by immunization with recently discovered carboxyl-terminal fragments of human parathyrin extracted from parathyroid tumors. Here, we report results of radioimmunoassays of nearly 200 normal and pathological sera with one of these antisera; we observed almost complete differentiation between concentrations of parathyrin in serum of healthy normal subjects and patients with primary, secondary (due to chronic renal failure), or "ectopic" hyperparathyroidism (due to nonparathyroid cancer). The availability of a new immunogen should now make possible the deliberate production of large quantities of diagnostically applicable parathyrin antisera directed toward the carboxyl-terminal region of human parathyrin. This should, in turn, lead to more widespread availability of this useful radioimmunoassay.

Characterization of a novel parathyroid hormone (PTH) receptor with specificity for the carboxyl-terminal region of PTH-(1-84)

Endocrinology ◽  
1995 ◽  
Vol 136 (11) ◽  
pp. 4732-4740 ◽  
Author(s):  
N Inomata ◽  
M Akiyama ◽  
N Kubota ◽  
H Jüppner
Keyword(s):  
Parathyroid Hormone ◽  
Terminal Region ◽  
Carboxyl Terminal

IMMUNOLOGICAL PROPERTIES OF SYNTHETIC HUMAN PARATHYROID HORMONE 53–84 FRAGMENT

1979 ◽  
Vol 80 (1) ◽  
pp. 153-155 ◽  
Author(s):  
G. N. HENDY ◽  
R. M. MANNING ◽  
M. ROSENBLATT ◽  
G. W. TREGEAR ◽  
H. T. KEUTMANN ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Synthetic Peptide ◽  
Terminal Region ◽  
Human Parathyroid Hormone ◽  
Enzymic Digestion ◽  
Immunological Properties ◽  
Carboxyl Terminal

The immunological properties of a synthetic peptide comprising the carboxyl-terminal 53–84 region of human parathyroid hormone (PTH) have been studied. The immunoreactivity of the synthetic human PTH-(53–84) peptide paralleled that of a 53–84 fragment of the native human hormone prepared by enzymic digestion, in both a standard radioimmunoassay, which was not region-specific, and also a radioimmunoassay specific for the carboxyl-terminal region of PTH. However, in both types of radioimmunoassay the synthetic human PTH-(53–84) peptide was four to five times more reactive than the native human PTH-(53–84) fragment.

Human Parathyroid Hormone: Synthesis and Chemical, Biological, and Immunological Evaluation of the Carboxyl-Terminal Region*

Endocrinology ◽  
1978 ◽  
Vol 103 (3) ◽  
pp. 978-984 ◽  
Author(s):  
MICHAEL ROSENBLATT ◽  
GINO V. SEGRE ◽  
GEOFFREY W. TREGEAR ◽  
GARY L. SHEPARD ◽  
GEORGE A. TYLER ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Terminal Region ◽  
Human Parathyroid Hormone ◽  
Hormone Synthesis ◽  
Carboxyl Terminal ◽  
Immunological Evaluation
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