Parathyroid hormone secretory function and serum levels of carboxyl-terminal flanking peptide (PDN-21) of the human calcitonin gene in normal subjects and osteoporotic patients

1986 ◽  
Vol 113 (1_Suppl) ◽  
pp. S185-S186
Author(s):  
J. ITTNER ◽  
M.A. DAMBACHER ◽  
R. MUFF ◽  
J.A. FISCHER
Keyword(s):  
Parathyroid Hormone ◽  
Normal Subjects ◽  
Serum Levels ◽  
Human Calcitonin ◽  
Secretory Function ◽  
Calcitonin Gene ◽  
Carboxyl Terminal

Parathyrin radioimmunoassay: diagnostic utility of antisera produced against carboxyl-terminal fragments of the hormone from the human.

1978 ◽  
Vol 24 (3) ◽  
pp. 451-454 ◽  
Author(s):  
F P Di Bella ◽  
J M Kehrwald ◽  
K Laakso ◽  
L Zitzner
Keyword(s):  
Renal Failure ◽  
Parathyroid Hormone ◽  
Chronic Renal Failure ◽  
Guinea Pigs ◽  
Normal Subjects ◽  
Terminal Region ◽  
Diagnostic Utility ◽  
Human Origin ◽  
Widespread Availability ◽  
Carboxyl Terminal

Abstract Antisera directed toward the carboxyl-terminal region of human parathyrin (parathyroid hormone), for use in daignostically applicable radioimmunoassays of the hormone in serum, are scarce, largely because of the lack of suitable immunogens of human origin. We produced four antisera in goats and guinea pigs by immunization with recently discovered carboxyl-terminal fragments of human parathyrin extracted from parathyroid tumors. Here, we report results of radioimmunoassays of nearly 200 normal and pathological sera with one of these antisera; we observed almost complete differentiation between concentrations of parathyrin in serum of healthy normal subjects and patients with primary, secondary (due to chronic renal failure), or "ectopic" hyperparathyroidism (due to nonparathyroid cancer). The availability of a new immunogen should now make possible the deliberate production of large quantities of diagnostically applicable parathyrin antisera directed toward the carboxyl-terminal region of human parathyrin. This should, in turn, lead to more widespread availability of this useful radioimmunoassay.

Circulating levels of calciotropic hormones during treatment with nasal salmon calcitonin

1991 ◽  
Vol 125 (2) ◽  
pp. 127-131 ◽  
Author(s):  
Gorm Thamsborg ◽  
Tommy L. Storm ◽  
Henrik Daugaard ◽  
Søren Schifter ◽  
Ole H. Sørensen
Keyword(s):  
Vitamin D ◽  
Parathyroid Hormone ◽  
Nasal Spray ◽  
Salmon Calcitonin ◽  
Serum Levels ◽  
Treated Group ◽  
Human Calcitonin ◽  
Vitamin D Metabolites ◽  
Calciotropic Hormones ◽  
Circulating Levels

Abstract. Circulating levels of calciotropic hormones were measured during one year of treatment with either 200 IU of salmon calcitonin daily or placebo as a nasal spray in 20 postmenopausal women with a former Colles' fracture. A supplement of 0.5 gram elemental calcium was given to all participants. Serum levels of parathyroid hormone and human calcitonin were determined with radioimmunoassays, and serum levels of vitamin D metabolites were determined with protein binding assays. We did not find any significant differences between the two groups with respect to serum levels of calciotropic hormones. In the salmon calcitonin treated group there was a tendency towards a small decrease in serum levels of human calcitonin and an increase in serum levels of calcitriol. Our results suggest that treatment with 200 IU of salmon calcitonin daily as a nasal spray does not markedly affect fasting serum levels of parathyroid hormone, human calcitonin, and vitamin D metabolitis.

Human calcitonin gene related peptide: a potent endogenous vasodilator in man

1986 ◽  
Vol 70 (4) ◽  
pp. 389-393 ◽  
Author(s):  
A. D. Struthers ◽  
M. J. Brown ◽  
D. W. R. Macdonald ◽  
J. L. Beacham ◽  
J. C. Stevenson ◽  
...  
Keyword(s):  
Diastolic Pressure ◽  
Normal Subjects ◽  
Calcitonin Gene Related Peptide ◽  
Plasma Calcium ◽  
Human Calcitonin ◽  
High Concentration ◽  
Plasma Adrenaline ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Perivascular Nerves

1. In addition to calcitonin and katacalcin, it is now known that the human calcitonin gene encodes a novel peptide called calcitonin gene related peptide (CGRP). In experimental animals, CGRP produces vasodilatation and complex changes in plasma calcium. 2. We have now assessed its biological activity in man by infusing human CGRP (hCGRP) into six normal volunteers. hCGRP (545 pmol/min) caused the diastolic pressure to fall from 64 ± 5 to 55 ± 7mmHg (P < 0.05), the heart rate to increase from 61 ± 7 to 87 ± 5 beats/min (P < 0.05) and the skin temperature to increase from 33.7 ± 0.9 to 34.9 ± 0.5°C. Plasma noradrenaline increased from 481 ± 126 to 835 ± 65 pg/ml (P < 0.05) and plasma adrenaline from 57 ± 17 to 82 ± 12 pg/ml (P < 0.05). There were no significant changes in the albumin-corrected plasma calcium. 3. hCGRP is thus a potent endogenous vasodilator in man and is in fact more potent than any other known vasodilator. Together with the observations that CGRP circulates in normal subjects at relatively high concentration (approximately 25 pmol/l) and that CGRP is present in perivascular nerves, this study suggests a possible role for CGRP in controlling peripheral vascular tone in man.

Diagnostic Evaluation of Measurements of Carboxyl-Terminal Flanking Peptide (PDN-21) of the Human Calcitonin Gene in Human Serum*

1985 ◽  
Vol 61 (6) ◽  
pp. 1133-1137 ◽  
Author(s):  
J. ITTNER ◽  
M.A. DAMBACHER ◽  
W. BORN ◽  
J-M. KETELSLEGERS ◽  
M. BUYSSCHAERT ◽  
...  
Keyword(s):  
Human Serum ◽  
Diagnostic Evaluation ◽  
Human Calcitonin ◽  
Calcitonin Gene ◽  
Carboxyl Terminal

scholarly journals Short carboxyl terminal parathyroid hormone peptides modulate human parathyroid hormone signaling in mouse osteoblasts

2021 ◽  
Vol 572 ◽  
pp. 15-19
Author(s):  
Kittrawee Kritmetapak ◽  
Ravinder J. Singh ◽  
Theodore A. Craig ◽  
Jolaine M. Hines ◽  
Rajiv Kumar
Keyword(s):  
Parathyroid Hormone ◽  
Hormone Signaling ◽  
Human Parathyroid Hormone ◽  
Carboxyl Terminal

scholarly journals Calcitonin gene-related peptide receptor antagonist human CGRP-(8-37)

1989 ◽  
Vol 256 (2) ◽  
pp. E331-E335 ◽  
Author(s):  
T. Chiba ◽  
A. Yamaguchi ◽  
T. Yamatani ◽  
A. Nakamura ◽  
T. Morishita ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Adenylate Cyclase ◽  
Calcitonin Gene Related Peptide ◽  
Human Calcitonin ◽  
Liver Plasma Membrane ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Liver Membranes ◽  
Calcitonin Receptors ◽  
Stimulation Of

From this study, we predicted that the human calcitonin gene-related peptide (hCGRP) fragment hCGRP-(8-37) would be a selective antagonist for CGRP receptors but an agonist for calcitonin (CT) receptors. In rat liver plasma membrane, where CGRP receptors predominate and CT appears to act through these receptors, hCGRP-(8-37) dose dependently displaced 125I-[Tyr0]rat CGRP binding. However, hCGRP-(8-37) had no effect on adenylate cyclase activity in liver plasma membrane. Furthermore, hCGRP-(8-37) inhibited adenylate cyclase activation induced not only by hCGRP but also by hCT. On the other hand, in LLC-PK1 cells, where calcitonin receptors are abundant and CGRP appears to act via these receptors, the bindings of 125I-[Tyr0]rat CGRP and 125I-hCT were both inhibited by hCGRP-(8-37). In contrast to liver membranes, interaction of hCGRP-(8-37) with these receptors led to stimulation of adenosine 3',5'-cyclic monophosphate (cAMP) production in LLC-PK1 cells, and moreover, this fragment did not inhibit the increased production of cAMP induced not only by hCT but also by hCGRP. Thus hCGRP-(8-37) appears to be a useful tool for determining whether the action of CGRP as well as that of CT is mediated via specific CGRP receptors or CT receptors.

Differences in postexercise serum levels of parathyroid hormone and osteocalcin before and after 6 weeks of endurance training in elderly men

1996 ◽  
Vol 6 (S1) ◽  
pp. 296-296
Author(s):  
E. Zerath ◽  
X. Holy ◽  
P. Douce ◽  
C. Y. Guezennec ◽  
J. C. Chatard
Keyword(s):  
Parathyroid Hormone ◽  
Endurance Training ◽  
Serum Levels ◽  
Elderly Men ◽  
Before And After
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