Determination and expression of platelet-derived growth factor-AA in bone cell cultures.

Endocrinology ◽  
1992 ◽  
Vol 130 (4) ◽  
pp. 1916-1922
Author(s):  
S Rydziel ◽  
C Ladd ◽  
T L McCarthy ◽  
M Centrella ◽  
E Canalis
Keyword(s):  
Growth Factor ◽  
Cell Cultures ◽  
Bone Cell ◽  
Platelet Derived Growth Factor ◽  
Bone Cell Cultures

Recombinant transforming growth factor type beta 3: biological activities and receptor-binding properties in isolated bone cells

1990 ◽  
Vol 10 (9) ◽  
pp. 4473-4479
Author(s):  
P ten Dijke ◽  
K K Iwata ◽  
C Goddard ◽  
C Pieler ◽  
E Canalis ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Cultures ◽  
Binding Sites ◽  
Transforming Growth Factor ◽  
Bone Cell ◽  
Scatchard Analysis ◽  
Tgf Beta ◽  
Surface Binding ◽  
Factor Type ◽  
Bone Cell Cultures

We have recently cloned the cDNA for transforming growth factor type beta 3 (TGF-beta 3), a new member of the TGF-beta gene family. We examined the biological effects of recombinant TGF-beta 3 protein in osteoblast-enriched bone cell cultures. In this report we demonstrate that TGF-beta 3 is a potent regulator of functions associated with bone formation, i.e., mitogenesis, collagen synthesis, and alkaline phosphatase activity. In a direct comparison between TGF-beta 3 and TGF-beta 1, TGF-beta 3 appeared to be three- to fivefold more potent than TGF-beta 1. Our cross-linking experiments with iodinated TGF-beta showed that in osteoblast-enriched bone cell cultures, both TGF-beta 3 and TGF-beta 1 associated with the same three cell surface binding sites. Scatchard analysis of receptor competition studies indicated the presence of high-affinity binding sites for TGF-beta 3 in the picomolar range. TGF-beta 3 showed an approximately fourfold-higher apparent affinity than TGF-beta 1 in overall binding.

Growth factors regulate the synthesis of insulin-like growth factor-I in bone cell cultures.

Endocrinology ◽  
1993 ◽  
Vol 133 (1) ◽  
pp. 33-38 ◽  
Author(s):  
E Canalis ◽  
J Pash ◽  
B Gabbitas ◽  
S Rydziel ◽  
S Varghese
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Cell Cultures ◽  
Bone Cell ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Growth Factor I ◽  
Bone Cell Cultures

scholarly journals Recombinant transforming growth factor type beta 3: biological activities and receptor-binding properties in isolated bone cells.

1990 ◽  
Vol 10 (9) ◽  
pp. 4473-4479 ◽  
Author(s):  
P ten Dijke ◽  
K K Iwata ◽  
C Goddard ◽  
C Pieler ◽  
E Canalis ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Cultures ◽  
Binding Sites ◽  
Transforming Growth Factor ◽  
Bone Cell ◽  
Scatchard Analysis ◽  
Tgf Beta ◽  
Surface Binding ◽  
Factor Type ◽  
Bone Cell Cultures

We have recently cloned the cDNA for transforming growth factor type beta 3 (TGF-beta 3), a new member of the TGF-beta gene family. We examined the biological effects of recombinant TGF-beta 3 protein in osteoblast-enriched bone cell cultures. In this report we demonstrate that TGF-beta 3 is a potent regulator of functions associated with bone formation, i.e., mitogenesis, collagen synthesis, and alkaline phosphatase activity. In a direct comparison between TGF-beta 3 and TGF-beta 1, TGF-beta 3 appeared to be three- to fivefold more potent than TGF-beta 1. Our cross-linking experiments with iodinated TGF-beta showed that in osteoblast-enriched bone cell cultures, both TGF-beta 3 and TGF-beta 1 associated with the same three cell surface binding sites. Scatchard analysis of receptor competition studies indicated the presence of high-affinity binding sites for TGF-beta 3 in the picomolar range. TGF-beta 3 showed an approximately fourfold-higher apparent affinity than TGF-beta 1 in overall binding.

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