Competitive Ligand-Binding Assay for Measurement of Sex Hormone-Binding Globulin (SHBG)

1973 ◽  
Vol 37 (6) ◽  
pp. 873-881 ◽  
Author(s):  
DAN TULCHINSKY ◽  
INDER J. CHOPRA
Keyword(s):  
Ligand Binding ◽  
Binding Assay ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Ligand Binding Assay ◽  
Hormone Binding ◽  
Competitive Ligand

A Competitive Ligand Binding Assay for Measurement of Thyroid Hormone-Binding Inhibitor in Serum and Tissues*

1984 ◽  
Vol 58 (4) ◽  
pp. 619-628 ◽  
Author(s):  
INDER J. CHOPRA ◽  
TIEN-SHANG HUANG ◽  
ROBERT E. HURD ◽  
ANGELITA BEREDO ◽  
DAVID H. SOLOMON
Keyword(s):  
Thyroid Hormone ◽  
Ligand Binding ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Hormone Binding ◽  
Competitive Ligand

TBG MEASUREMENTS BY COMPETITIVE LIGAND BINDING ASSAY (CLBA)

1975 ◽  
Vol 80 (1_Suppla) ◽  
pp. S15
Author(s):  
K. H. Rudorff ◽  
H. J. Kröll ◽  
J. Herrmann
Keyword(s):  
Ligand Binding ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Competitive Ligand

A Method for the Determination of Sex Hormone Binding Globulin Using Concanavalin A-Sepharose

1992 ◽  
Vol 29 (2) ◽  
pp. 168-171 ◽  
Author(s):  
J A Whittaker ◽  
M L Cawood ◽  
R E Oakey
Keyword(s):  
Concanavalin A ◽  
Binding Assay ◽  
Solid Phase ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Research Use ◽  
Hormone Binding

A binding assay for sex hormone binding globulin (SHBG) has been developed in which SHBG is saturated with tritiated dihydrotestosterone (DHT). Separation of bound and free DHT is achieved by using Concanavalin A-Sepharose as a solid phase matrix. The method is described and its performance, including linearity, imprecision and comparison with other methods, is assessed. The assay is simple and robust and is suitable for analysis of samples of plasma or serum for clinical or research use.

scholarly journals Interaction of Lignans with Human Sex Hormone Binding Globulin (SHBG)

1997 ◽  
Vol 52 (11-12) ◽  
pp. 834-843 ◽  
Author(s):  
Matthias Schöttner ◽  
Dietmar Ganßer ◽  
Gerhard Spitelle
Keyword(s):  
Binding Affinity ◽  
Binding Assay ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Protecting Groups ◽  
Hormone Binding ◽  
Substitution Pattern ◽  
Structure And Activity

Abstract Lignans bind to sex hormone-binding globulin (SHBG ). The lignan with the highest binding affinity is (±)-3,4-divanillyltetrahydrofuran. In a double Stobbe condensation - without use of protecting groups - a wide variety of lignans with different substitution pattern in the aromatic and aliphatic part of the molecule was synthesized. These lignans were tested in a SHBG -binding assay which allowed to deduce the following relationship between structure and activity: 1) (±)-diastereoisomers are more active than meso compounds 2.) the 4-hydroxy- 3-methoxy (guajacyl) substitution pattern in the aromatic part is most effective 3.) the activity increases with the decline in polarity of the aliphatic part of the molecule.

Syntheses and ligand-binding studies of 1α- and 17α-aminoalkyl dihydrotestosterone derivatives to human sex hormone-binding globulin

Steroids ◽  
2003 ◽  
Vol 68 (7-8) ◽  
pp. 629-639 ◽  
Author(s):  
Hagen Hauptmann ◽  
Jochen Metzger ◽  
Andreas Schnitzbauer ◽  
Claude Y Cuilleron ◽  
Elisabeth Mappus ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
Binding Studies
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