scholarly journals The spindle pole body of Aspergillus nidulans is asymmetrical and contains changing numbers of γ-tubulin complexes

2019 ◽  
Vol 132 (24) ◽  
pp. jcs234799 ◽  
Author(s):  
Xiaolei Gao ◽  
Marjorie Schmid ◽  
Ying Zhang ◽  
Sayumi Fukuda ◽  
Norio Takeshita ◽  
...  
Keyword(s):  
Aspergillus Nidulans ◽  
Spindle Pole Body ◽  
Spindle Pole ◽  
Pole Body

scholarly journals The spindle pole-body localization of activated cytoplasmic dynein is cell cycle-dependent in Aspergillus nidulans

2021 ◽  
Vol 148 ◽  
pp. 103519
Author(s):  
Baronger Dowell Bieger ◽  
Aysha H. Osmani ◽  
Xin Xiang ◽  
Martin J. Egan
Keyword(s):  
Cell Cycle ◽  
Aspergillus Nidulans ◽  
Spindle Pole Body ◽  
Cytoplasmic Dynein ◽  
Spindle Pole ◽  
Pole Body ◽  
Cycle Dependent

γ-tubulin is a component of the spindle pole body that is essential for microtubule function in Aspergillus nidulans

Cell ◽  
1990 ◽  
Vol 61 (7) ◽  
pp. 1289-1301 ◽  
Author(s):  
Berl R. Oakley ◽  
C.Elizabeth Oakley ◽  
Yisang Yoon ◽  
M.Katherine Jung
Keyword(s):  
Aspergillus Nidulans ◽  
Spindle Pole Body ◽  
Spindle Pole ◽  
Pole Body ◽  
Microtubule Function

scholarly journals BIMA, a TPR-containing protein required for mitosis, localizes to the spindle pole body in Aspergillus nidulans.

1993 ◽  
Vol 120 (4) ◽  
pp. 959-968 ◽  
Author(s):  
P M Mirabito ◽  
N R Morris
Keyword(s):  
Aspergillus Nidulans ◽  
Spindle Pole Body ◽  
Apparent Molecular Weight ◽  
Spindle Pole ◽  
Nuclear Staining ◽  
Loss Of Function ◽  
Independent Manner ◽  
Pole Body ◽  
A Cell ◽  
Arrest Growth

The Aspergillus nidulans bimA gene is required for mitosis. Loss of function mutations in bimA cause cells to arrest growth with condensed chromatin and a short, metaphaselike mitotic spindle. bimA is a member of a gene family defined by a repeated motif called the Tetratrico Peptide Repeat (TPR), which is found in genes from bacteria, yeast and insects. Several yeast TPR genes are also required for mitosis, including Saccharomyces cerevisiae CDC27 and Schizosaccharomyces pombe nuc2+, which appear to be functional homologs of bimA. We have developed antisera specific to the bimA protein (BIMA) and have characterized BIMA by western blot and immunocytochemical analyses. BIMA is heterogeneous in apparent molecular weight, consisting of a major 90-kD species and at least two minor species of approximately 105 kD. The results of BIMA localization by immunofluorescence microscopy depend on the level of BIMA expression. Overexpression of BIMA, which had no deleterious affect on growth or mitosis, resulted in localization of BIMA on or throughout most nuclei. Nuclear staining was granular, and overlapped but was not completely coincident with DNA staining by DAPI. In contrast, when expressed at normal levels, BIMA colocalized with the spindle pole body (SPB). BIMA localized to the SPB in a cell cycle independent manner. These results show that BIMA is either associated with or is a component of the SPB, and they suggest that BIMA functions at the spindle poles to promote the onset of anaphase.

Sign in / Sign up

Export Citation Format

Share Document