scholarly journals Physical Mapping of the 18S Ribosomal RNA Gene of Peach (Prunus persica (L.) Batsch) Chromosomes by Fluorescence in situ Hybridization.

1999 ◽  
Vol 49 (1) ◽  
pp. 49-51 ◽  
Author(s):  
Masashi Yamamoto ◽  
Takehiko Shimada ◽  
Takashi Haji ◽  
Nobuko Mase ◽  
Yoshihiko Sato
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Rna ◽  
Physical Mapping ◽  
Prunus Persica ◽  
Ribosomal Rna Gene ◽  
18S Ribosomal Rna ◽  
18S Ribosomal Rna Gene

scholarly journals Detection of the Ribosomal RNA Gene in Pear (Pyrus spp.) using Fluorescence in situ Hybridization

2010 ◽  
Vol 79 (4) ◽  
pp. 335-339 ◽  
Author(s):  
Masashi Yamamoto ◽  
Shingo Terakami ◽  
Toshiya Yamamoto ◽  
Norio Takada ◽  
Tatsuya Kubo ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Rna ◽  
Ribosomal Rna Gene

scholarly journals Phylogenetic analysis based on 18S ribosomal RNA gene sequences supports the existence of class polyacanthocephala (acanthocephala)

2002 ◽  
Vol 23 (2) ◽  
pp. 288-292 ◽  
Author(s):  
Martı́n Garcı́a-Varela ◽  
Michael P Cummings ◽  
Gerardo Pérez-Ponce de León ◽  
Scott L Gardner ◽  
Juan P Laclette
Keyword(s):  
Phylogenetic Analysis ◽  
Ribosomal Rna ◽  
Gene Sequences ◽  
Ribosomal Rna Gene ◽  
18S Ribosomal Rna ◽  
18S Ribosomal Rna Gene

scholarly journals Application of PCR-RFLP and MASA Analyses on 18S Ribosomal RNA Gene Sequence for the Identification of Three Ginseng Drugs.

1997 ◽  
Vol 20 (7) ◽  
pp. 765-769 ◽  
Author(s):  
Hirotoshi FUSHIMI ◽  
Katsuko KOMATSU ◽  
Masaharu ISOBE ◽  
Tsuneo NAMBA
Keyword(s):  
Ribosomal Rna ◽  
Gene Sequence ◽  
Ribosomal Rna Gene ◽  
18S Ribosomal Rna ◽  
18S Ribosomal Rna Gene ◽  
Pcr Rflp

Detection of rRNA and phaseolin genes on polytene chromosomes of Phaseolus coccineus by fluorescence in situ hybridization after pepsin pretreatment

Genome ◽  
1994 ◽  
Vol 37 (6) ◽  
pp. 1018-1021 ◽  
Author(s):  
M. Nenno ◽  
K. Schumann ◽  
W. Nagl
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Rna ◽  
Storage Protein ◽  
Seed Storage Protein ◽  
Polytene Chromosomes ◽  
Seed Storage ◽  
Phaseolus Coccineus ◽  
Rna Genes

This is the first report of fluorescence in situ hybridization (FISH) on plant polytene chromosomes. Different protease pretreatments have been tested to improve fluorescence in situ hybridization FISH on polytene chromosomes of a plant, Phaseolus coccineus, with the aim to enable the detection of low-copy genes. The structural preservation of the chromosomes and the distinctness of the FISH signals were comparatively analysed with a probe for the ribosomal RNA genes after digestion with pepsin and trypsin. The pepsin pretreatment resulted in a general loosening of chromatin with good conservation of chromosome morphology and an increased number and density of signal points. The six nucleolus organizers exhibited significant differences in condensation. The pretreatment with pepsin enabled the detection of the low-copy genes encoding the seed storage protein phaseolin.Key words: plant, Leguminosae, ribosomal RNA genes, seed storage protein genes, protease.

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