A COMPARISON OF NOMARSKI DIFFERENTIAL INTERFERENCE CONTRAST AND PHASE CONTRAST MICROSCOPY OF THE GUINEA PIG ORGAN OF CORTI

1971 ◽  
Vol 81 (7) ◽  
pp. 1090-1118 ◽  
Author(s):  
Frederick A. Kuhn ◽  
Ruediger Thalmann ◽  
William F. Marovitz
Keyword(s):  
Guinea Pig ◽  
Phase Contrast ◽  
Organ Of Corti ◽  
Phase Contrast Microscopy ◽  
Differential Interference Contrast ◽  
Nomarski Differential Interference Contrast ◽  
Contrast Microscopy

Quantitative Visible-Light and Electron Phase Microscopy

1998 ◽  
Vol 4 (S2) ◽  
pp. 408-409
Author(s):  
A. Barty ◽  
K.A. Nugent ◽  
D. Paganin ◽  
A Roberts
Keyword(s):  
Phase Structure ◽  
Quantitative Data ◽  
Phase Contrast ◽  
Phase Distribution ◽  
Differential Interference Contrast ◽  
Phase Microscopy ◽  
Intensity Measurements ◽  
Nomarski Differential Interference Contrast ◽  
Contrast Microscopy ◽  
The University

At the University of Melbourne we have been pursuing an ongoing program of investigation into the recovery of phase information from intensity measurements [1-9]. In this paper we consider the implications of this work in optical and electron microscopy.Many objects of interest to biologists are phase objects which means that light is slowed and refracted in the object but not absorbed. Techniques such as phase-contrast microscopy and Nomarski differential interference contrast (DIC) microscopy are traditionally used to render the phase structure visible but do not directly map the phase distribution and are unable to produce quantitative data. These techniques also entangle the phase and amplitude information and so have limitations where both phase and amplitude information is required. In this paper, we demonstrate quantitative non-interferometric recovery of microscopic phase structure using incoherent illumination of the type commonly found in optical microscopes, and show that our results correlate with structure observed using Nomarski DIC techniques.

The Bacteroides glycocalyx as visualized by differential interference contrast microscopy

1988 ◽  
Vol 34 (11) ◽  
pp. 1189-1195 ◽  
Author(s):  
Dwight W. Lambe Jr. ◽  
Kaethe P. Ferguson ◽  
Donald A. Ferguson Jr.
Keyword(s):  
Phase Contrast ◽  
Agar Medium ◽  
Single Cells ◽  
Visual Observation ◽  
Phase Contrast Microscopy ◽  
Differential Interference Contrast ◽  
Differential Interference Contrast Microscopy ◽  
India Ink ◽  
Contrast Microscopy ◽  
Interference Contrast Microscopy

The glycocalyx of eight strains representing six species of Bacteroides was examined by differential interference contrast microscopy. Wet mounts in India ink were prepared from bacteria cultured in broth and on an agar medium; the wet mounts were observed by phase-contrast microscopy and differential interference contrast microscopy. With differential interference contrast microscopy, all bacteria demonstrated a glycocalyx, which included capsules surrounding single cells and microcolonies, strands of glycocalyx connecting cells and microcolonies, detached slime, and solid masses of glycocalyx in which innumerable bacteria were enmeshed. Bacteria showed comparable amounts of glycocalyx by visual observation with differential interference contrast microscopy whether grown on plates or in broth. Serial transfers of cultures did not diminish the amount of glycocalyx. Differential interference contrast microscopy proved to be a superior method to phase contrast for examining wet preparations of Bacteroides.

Use of phase contrast microscopy to determine the height of the organ of Corti in whole‐mount preparations

1987 ◽  
Vol 81 (5) ◽  
pp. 1499-1506 ◽  
Author(s):  
Patrick J. Baggot ◽  
Barbara A. Bohne ◽  
Denyse G. Bozzay ◽  
Gary W. Harding
Keyword(s):  
Phase Contrast ◽  
Organ Of Corti ◽  
Phase Contrast Microscopy ◽  
Whole Mount ◽  
Contrast Microscopy

scholarly journals Comparative Morphologic and Morphometric Study on the Developmental Aspects of In Vitro and In Vivo Reared Echino-coccus granulosus Sensu Stricto Using Differential Interference Contrast (DIC)/Nomarski and Phase Contrast Microscopy

2019 ◽  
Author(s):  
Seyedeh Faezeh SADJJADI ◽  
Mina MOTAMEDI ◽  
Tahereh MOHAMMADZADEH ◽  
Seyed Mahmoud SADJJADI
Keyword(s):  
Phase Contrast ◽  
Sensu Stricto ◽  
Whole Body ◽  
Phase Contrast Microscopy ◽  
Differential Interference Contrast ◽  
Morphometric Characters ◽  
Reproductive Structures ◽  
Contrast Microscopy

Background: Echinococcus granulosus is a zoonotic parasite with worldwide distribution. The present study focused on comparative morphologic and morphometric observations on the developmental aspects of whole body, more special the reproductive structures of in vitro reared adult worms (RAW) and in vivo reared adult worms in definitive host (AWIDH) using differential interference contrast (DIC)/Nomarski, phase contrast and routine optical microscopy. Methods: A total number of 10 in vitro and 10 in vivo reared adult worms of E. granulosus sensu stricto, G1 strain were selected. The worms were processed by Formaldehyde-Alcohol-Azocarmine-Lactophenol (FAAL). The details of morphological factors and reproductive structures of each worm including 25 biometrical parameters were studied by routine optical, phase contrast and Nomarski microscopy. The details of the samples were photographed, measured and analyzed. The fine structures of the parasite including the details of cirrus sac and developmental stages in different strobila were more obvious observing by Nomarski microscopy.  Results: The morphometric characters in the RAW and AWIDH showed that length of immature proglottid, length of mature proglottid, length of suckers are larger in RAW than AWIDH worms with statistical difference. Characters in E. granulosus of RAW and AWIDH showed that total number of segments, number of mature segments and the total number of testes were greater in RAW than AWIDH worms; while only the number of mature segments was statistically different is two groups. Conclusion: Application of DIC/Nomarski and phase contrast microscopy together with morphometric criteria are useful means for comparing the developmental aspects of in vitro and in vivo reared adults of E. granulosus.

Platelet Aggregation in Mammalians (Human, Rat, Rabbit, Guinea-Pig, Horse, Dog) A Comparative Study

1967 ◽  
Vol 17 (01/02) ◽  
pp. 099-111 ◽  
Author(s):  
Z Sinakos ◽  
J. P Caen
Keyword(s):  
Platelet Aggregation ◽  
Guinea Pig ◽  
Comparative Study ◽  
Phase Contrast ◽  
Photometric Method ◽  
Phase Contrast Microscopy ◽  
Bovine Thrombin ◽  
Contrast Microscopy ◽  
Induced Aggregation

SummaryA comparative study of mammalian (human, rat, rabbit, guinea-pig, horse, dog) platelet aggregation was done using phase contrast microscopy and photometric method and gave us the following results :1. The animal platelets do not spread spontaneously onto glass in contrast to human ones (in their own plasma).2. They do not aggregate in presence of adrenalin, noradrenalin, 5-HT but these amines increase the sensitivity of the platelets towards ADP.3. Although all the platelets aggregate in presence of ADP, bovine thrombin, collagen or thimerosal, there exists some quantitative differences from one species to another.4. Adenosine and AMP are inhibitors of ADP-induced aggregation for human, rabbit and dog platelets and not for guinea-pig and horse. As for the rat, if adenosine is not inhibitor, AMP can be inhibitor if used at strong doses.5. There exist great differences in the ability of the various platelet-poor plasmas studied to inactivate the aggregating property of ADP, as for instance the weakest being the human one and the strongest the rat one.6. The authors insist on the importance of ADP-inhibition by AMP and adenosine, and the ADP-inactivation by the plasmas for the mammalian platelet aggregation and almost disaggregation.

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