scholarly journals Retraction: Incidence Study of Brucella abortus and Brucella melitensis in Bovine and Buffalo Semen Samples by Real-Time PCR Assay in Iran

2012 ◽  
Author(s):  
Farhad SAFARPOOR DEHKORDI
Keyword(s):  
Real Time ◽  
Incidence Study ◽  
Brucella Abortus ◽  
Real Time Pcr ◽  
Brucella Melitensis ◽  
Pcr Assay ◽  
Buffalo Semen

scholarly journals PP-040 Real-time PCR assay for identification of Brucella abortus in bulk milk samples in Iran

2010 ◽  
Vol 14 ◽  
pp. S36-S37 ◽  
Author(s):  
S. Moshkelani ◽  
M. Javaheri Koupaei ◽  
S. Rabiei ◽  
A. Doosti
Keyword(s):  
Real Time ◽  
Brucella Abortus ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Milk Samples ◽  
Bulk Milk

scholarly journals Evidence of Brucella abortus in Non-Preferred Caprine and Ovine Hosts by Real-time PCR Assay

2019 ◽  
Vol 51 (3) ◽  
Author(s):  
Muhammad Zain Saleem ◽  
Raheela Akhtar ◽  
Asim Aslam ◽  
Muhammad Imran Rashid ◽  
Zafar Iqbal Chaudhry ◽  
...  
Keyword(s):  
Real Time ◽  
Brucella Abortus ◽  
Real Time Pcr ◽  
Pcr Assay

scholarly journals Detection of Brucella Abortus in Caprine and Ovine by Real-Time PCR Assay

2021 ◽  
Vol 9 (5) ◽  
pp. 141
Author(s):  
Muhammad Bilawal Arain ◽  
Abdullah Babar ◽  
Muhammad Ibrahim Panhwar ◽  
Khush Hal ◽  
Muhammad Mubashir Farooq ◽  
...  
Keyword(s):  
Real Time ◽  
Brucella Abortus ◽  
Real Time Pcr ◽  
Pcr Assay

Real time PCR assay for differentiation of Brucella abortus and Brucella melitensis

2017 ◽  
Author(s):  
Vinay Kumar ◽  
Sushila Maan ◽  
Aman Kumar ◽  
Kanisht Batra ◽  
Deepika Deepika ◽  
...  
Keyword(s):  
Real Time ◽  
Brucella Abortus ◽  
Real Time Pcr ◽  
Brucella Melitensis ◽  
Economic Losses ◽  
Conventional Pcr ◽  
Serological Tests ◽  
Detectable Difference ◽  
Pcr Assays ◽  
Respective Species

Brucellosis is one of the zoonotic diseases of major concern and can cause huge economic losses to livestock industry. Serological tests and bacterial isolation are considered as the gold standard assay for diagnosis of Brucella spp. but they are time-consuming, hazardous and lack specificity. To control and eradicate a disease, a confirmatory diagnostic method which is sensitive, quick and specific is the foremost requirement. Therefore in this study, we evaluated the performances of two newly designed TaqMan real-time PCR assays targeting the BruAB_0168 gene and BMEII0466 gene for Brucella abortus and Brucella melitensis (respectively). Both the assays were found to be highly specific in differentiation of respective species. Both the assays can detect as low as 0.02 fg of DNA and there was no detectable difference found in sensitivity of these two tests. R2 value and efficiency of these tests ranged from 0.992 - 0.998 and 100- 106%, respectively showing that these assays are highly efficient. Compared to conventional PCR assays these qPCR assays were 100 times higher sensitive. In conclusion, the present study showed that the developed real-time PCR assays are more sensitive, specific, have high reproducibility and repeatability and are faster than serological and conventional PCR methods for differentiation of Brucella abortus and Brucella melitensis.

Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique

2016 ◽  
Vol 2 (1) ◽  
pp. 7 ◽  
Author(s):  
Yaran Majid ◽  
Najafi Somayeh ◽  
Shoaei Parisa ◽  
Ataei Behrooz ◽  
Fadaei Reza ◽  
...  
Keyword(s):  
Real Time ◽  
Brucella Abortus ◽  
Real Time Pcr ◽  
Brucella Melitensis ◽  
Dairy Product ◽  
Raw Milk

scholarly journals A novel real-time PCR assay for specific detection of Brucella melitensis

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Rene Kaden ◽  
Sevinc Ferrari ◽  
Erik Alm ◽  
Tara Wahab
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Brucella Melitensis ◽  
Specific Detection ◽  
Pcr Assay
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