Defocus-based three-dimensional particle location with extended depth of field via color coding

2019 ◽  
Vol 58 (17) ◽  
pp. 4734
Author(s):  
Zhaolou Cao ◽  
Chunjie Zhai
2017 ◽  
Vol 392 ◽  
pp. 252-257 ◽  
Author(s):  
Zhaolou Cao ◽  
Chunjie Zhai ◽  
Jinhua Li ◽  
Fenglin Xian ◽  
Shixin Pei

2015 ◽  
Vol 23 (2) ◽  
pp. 787 ◽  
Author(s):  
Heng Li ◽  
Danni Chen ◽  
Gaixia Xu ◽  
Bin Yu ◽  
Hanben Niu

1999 ◽  
Vol 24 (4) ◽  
pp. 253 ◽  
Author(s):  
Daniel L. Marks ◽  
Ronald A. Stack ◽  
David J. Brady ◽  
Joseph van der Gracht

2018 ◽  
Vol 42 (3) ◽  
pp. 377-384 ◽  
Author(s):  
S. N. Koreshev ◽  
D. S. Smorodinov ◽  
O. V. Nikanorov ◽  
M. A. Frolova

A quantitative evaluation of the depth of field of optical systems is given. Results of the calculation of the distribution of the complex amplitude and intensity in a three-dimensional scattering pattern formed by the optical system for an on-axis point object are presented. The work was carried out as part of developing optical systems with an extended depth of field for a synthesized hologram of a point object located on a perpendicular constructed to the hologram center.


2012 ◽  
Vol 95 (11) ◽  
pp. 14-27 ◽  
Author(s):  
Keiichiro Kagawa ◽  
Kenji Yamada ◽  
Eiji Tanaka ◽  
Jun Tanida

Author(s):  
A.M. Jones ◽  
A. Max Fiskin

If the tilt of a specimen can be varied either by the strategy of observing identical particles orientated randomly or by use of a eucentric goniometer stage, three dimensional reconstruction procedures are available (l). If the specimens, such as small protein aggregates, lack periodicity, direct space methods compete favorably in ease of implementation with reconstruction by the Fourier (transform) space approach (2). Regardless of method, reconstruction is possible because useful specimen thicknesses are always much less than the depth of field in an electron microscope. Thus electron images record the amount of stain in columns of the object normal to the recording plates. For single particles, practical considerations dictate that the specimen be tilted precisely about a single axis. In so doing a reconstructed image is achieved serially from two-dimensional sections which in turn are generated by a series of back-to-front lines of projection data.


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