Polarization Characterization of LEDs by Stokes Parameters Measurements

Stokes parameters have been measured by using a polarimeter consisting of a rotating phase plate before a fixed polarizer for bullet-shaped red, green and blue LEDs at 3 different directions of 0°, 45° and 90° from the principal axis. The degree of polarization is minimum at the observation angle 0° (observed head-on) for all colors as expected but has non-zero values (1-9%). As for the possible cause for the partial polarization, it is likely to be brought by striae inside the transparent epoxy resin that can be easily visible. Data at observation angle 90° have features common for all colors; the degree of polarization is highest, the long axis azimuth of the polarization ellipse is nearly in the horizontal direction, and the ellipticity is small. These features can be explained as follows. At observation angle 90°, only small fraction of the beam emitted nearly horizontally is detected possibly through multireflection (the plane of incidence is in the vertical plane) inside the top- and bottom-surfaces (in the horizontal direction) of the chip substrate. Since the reflectance for s-polarization (horizontal component) is higher than that for p-polarization, the emerging beam becomes horizontally polarized. The hypotheses that geometrical asymmetry generates polarization is experimentally supported.

Fried’s coherence length measurement of dynamic Kolmogorov type turbulence using autocorrelation function

A new method to find Fried’s coherence length of a dynamic Kolmogorov type turbulence in laboratory environment is reported in this paper. This method utilises autocorrelation function obtained from the quantitative characteristics of a rotating pseudo random phase plate in one of the arms of Mach-Zehnder interferometer. Theoretical formalism and experimental verification are presented.

Structure of ABCB1/P-Glycoprotein in the Presence of the CFTR Potentiator Ivacaftor

ABCB1/P-glycoprotein is an ATP binding cassette transporter that is involved in the clearance of xenobiotics, and it affects the disposition of many drugs in the body. Conformational flexibility of the protein within the membrane is an intrinsic part of its mechanism of action, but this has made structural studies challenging. Here, we have studied different conformations of P-glycoprotein simultaneously in the presence of ivacaftor, a known competitive inhibitor. In order to conduct this, we used high contrast cryo-electron microscopy imaging with a Volta phase plate. We associate the presence of ivacaftor with the appearance of an additional density in one of the conformational states detected. The additional density is in the central aqueous cavity and is associated with a wider separation of the two halves of the transporter in the inward-facing state. Conformational changes to the nucleotide-binding domains are also observed and may help to explain the stimulation of ATPase activity that occurs when transported substrate is bound in many ATP binding cassette transporters.

Development of computer-controlled atmospheric pressure plasma structuring for 2D/3D pattern on fused silica

Fused silica with structured and continuous patterns is increasingly demanded in advanced imaging and illumination fields because of its excellent properties and functional performance. Atmospheric pressure plasma, based on pure chemical etching under atmospheric pressure, is developed as a promising fabrication technique for fused silica due to its deterministic high material removal rate, controllable removal imprint and no mechanical load. The stable and controllable Gaussian-shape removal function makes computer-controlled plasma tool potential to generate complex structures with high accuracy, efficiency and flexibility. In the paper, computer-controlled atmospheric pressure plasma structuring (APPS) is proposed to fabricate 2D/3D patterns on fused silica optics. The capacitively coupled APPS system with a double-layer plasma torch and its discharge characteristics are firstly developed. By means of multi-physics simulation and process investigation, the stable and controllable Gaussian-shape removal function can be achieved. Two different structuring modes, including discrete and continuous APPS, are explored for 2D/3D patterns. A series of structuring experiments show that different kinds of 2D patterns (including square lens array, hexagon lens array and groove array) as well as complex 3D phase plate patterns have been successfully fabricated, which validates the effectiveness of the proposed APPS of 2D/3D patterns on fused silica optics.

A modified multiplexed vortex helico-conical petal-like zone plate

A modified multiplexed vortex helico-conical petal-like zone plate (MMVHPZP) is proposed to generate a polygon-like beam or light-arm beam with an adjustable opening. The MMVHPZP consists of the modified helico-conical petal-like zone plate (MHPZP) with the topological charge l and exponent n, and the multiplexed vortex spiral phase plate (MVSPP) with the inner topological charge l1 and outer topological charge l2. Moreover, when l1 is equal or unequal to l2, the MMVHPZP has the adjustable polygon-like beam or light-arm beam, respectively. In addition, when n is small or large, the number of arms is equal to the absolute difference between l1 and l2 or the sum of one and the absolute difference between l1 and l2, respectively. Furthermore, for the different l1 or l2, the opening is constant. With the increase of the n or l, the opening is larger. When l1 is greater or less than l2, the rotation direction of arms is the anticlockwise or clockwise direction, respectively.

Simultaneous orientation and 3D localization microscopy with a Vortex point spread function

Estimating the orientation and 3D position of rotationally constrained emitters with localization microscopy typically requires polarization splitting or a large engineered Point Spread Function (PSF). Here we utilize a compact modified PSF for single molecule emitter imaging to estimate simultaneously the 3D position, dipole orientation, and degree of rotational constraint from a single 2D image. We use an affordable and commonly available phase plate, normally used for STED microscopy in the excitation light path, to alter the PSF in the emission light path. This resulting Vortex PSF does not require polarization splitting and has a compact PSF size, making it easy to implement and combine with localization microscopy techniques. In addition to a vectorial PSF fitting routine we calibrate for field-dependent aberrations which enables orientation and position estimation within 30% of the Cramér-Rao bound limit over a 66 μm field of view. We demonstrate this technique on reorienting single molecules adhered to the cover slip, λ-DNA with DNA intercalators using binding-activated localization microscopy, and we reveal periodicity on intertwined structures on supercoiled DNA.