Separation of Dietary Folates by Gradient Reversed-Phase HPLC: Comparison of Alternative and Conventional Silica-Based Stationary Phases

2005 ◽  
Vol 62 (1-2) ◽  
pp. 33-40 ◽  
Author(s):  
M. Johansson ◽  
J. Jastrebova ◽  
A. Grahn ◽  
M. Jägerstad
Keyword(s):  
Stationary Phases ◽  
Reversed Phase ◽  
Reversed Phase Hplc

Immobilized Polysaccharide-Based Stationary Phases for Enantioseparation in Normal Versus Reversed Phase HPLC

2014 ◽  
Vol 78 (13-14) ◽  
pp. 909-915 ◽  
Author(s):  
Radim Geryk ◽  
Květa Kalíková ◽  
Jiří Vozka ◽  
Eva Tesařová
Keyword(s):  
Stationary Phases ◽  
Reversed Phase ◽  
Reversed Phase Hplc

Chiral Chromatography Studies of Chemical Behavior of Cinacalcet on Polysaccharide Chiral Reversed-Phase HPLC Stationary Phases

2012 ◽  
Vol 95 (6) ◽  
pp. 1639-1643 ◽  
Author(s):  
Michal Douša ◽  
Jiřiacute; Břicháč
Keyword(s):  
Chiral Stationary Phase ◽  
Stationary Phases ◽  
Active Pharmaceutical Ingredient ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc ◽  
Chiral Chromatography ◽  
Chemical Behavior ◽  
Analytical Resolution

Abstract A rapid HPLC method for the analytical resolution of cinacalcet enantiomers was developed. Four chiral columns (two amylose and two cellulose type) were evaluated in RP systems. Excellent enantioseparation with a resolution of more than 6 was achieved on Chiralpak AY (amylose 5-chloro-2-methylphenylcarbamate chiral stationary phase) using 10 mM triethylamine (pH 8.0)–acetonitrile (40 + 60, v/v) mobile phase. Validation of the HPLC method, including linearity, LOD, LOQ, precision, accuracy, and selectivity, was performed according to the International Conference on Harmonization guidelines. The method was successfully applied for the determination of (S)-cinacalcet in enantiopure active pharmaceutical ingredient (R)-cinacalcet.

Chemically Bonded Silica Stationary Phases:  Synthesis, Physicochemical Characterization, and Molecular Mechanism of Reversed-Phase HPLC Retention

1997 ◽  
Vol 69 (16) ◽  
pp. 3277-3284 ◽  
Author(s):  
Bogusław Buszewski ◽  
Renata M. Gadzała-Kopciuch ◽  
Michał Markuszewski ◽  
Roman Kaliszan
Keyword(s):  
Molecular Mechanism ◽  
Stationary Phases ◽  
Physicochemical Characterization ◽  
Reversed Phase ◽  
Reversed Phase Hplc
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