Characterization of new N,O-dialkyl phosphoramidate-bonded stationary phases for reversed-phase HPLC – retention and selectivity

The Analyst ◽  
2013 ◽  
Vol 138 (18) ◽  
pp. 5221 ◽  
Author(s):  
Szymon Bocian ◽  
Magdalena Paca ◽  
Boguslaw Buszewski
Keyword(s):  
Stationary Phases ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Bonded Stationary Phases

scholarly journals Raman Spectroscopic Characterization of RP-18-Type Chemically Bonded Stationary Phases for Liquid Chromatography

1999 ◽  
Vol 82 (2) ◽  
pp. 297-304 ◽  
Author(s):  
Gabriela Kowalik ◽  
Krystyna Rogosz ◽  
Teresa Kowalska
Keyword(s):  
Raman Spectra ◽  
High Power ◽  
Stationary Phases ◽  
Spectroscopic Characterization ◽  
Reversed Phase ◽  
Neodymium Laser ◽  
Raman Spectroscopic ◽  
Bonded Stationary Phases ◽  
Laser Raman Spectra

Abstract This study is the first Raman spectroscopic characterization of conventional chemically bonded liquid chromatographic (LC) stationary phases of the RP-18 type using the high-power Nd:WV04 neodymium laser. Raman spectra were obtained for octadecyl (C18) chemically bonded silica-based stationary phases, as well as for silica, aminopropyl (NH2), cyanopropyl (CN), diol (OH), octyl (C8), and methyl (C1) phases for comparison. This study demonstrates that Raman spectroscopy using the high-power neodymium laser can be used to study RP-18-type LC stationary phases and to quantitate the density of coverage of the silica matrix with octadecyl ligands. The paper presents the experimental setup and conditions usedto obtain Raman spectra of commercial RP-18-type chemically bonded stationary phases and examines the spectroscopic similarities and differences observed for different bonded ligands typically used in reversed-phase and normal-phase LC separations.

Retention properties of novel β-CD bonded stationary phases in reversed-phase HPLC mode

Talanta ◽  
2009 ◽  
Vol 78 (3) ◽  
pp. 916-921 ◽  
Author(s):  
Yanyan Zhao ◽  
Zhimou Guo ◽  
Yongping Zhang ◽  
Xingya Xue ◽  
Qing Xu ◽  
...  
Keyword(s):  
Stationary Phases ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Bonded Stationary Phases

Immobilized Polysaccharide-Based Stationary Phases for Enantioseparation in Normal Versus Reversed Phase HPLC

2014 ◽  
Vol 78 (13-14) ◽  
pp. 909-915 ◽  
Author(s):  
Radim Geryk ◽  
Květa Kalíková ◽  
Jiří Vozka ◽  
Eva Tesařová
Keyword(s):  
Stationary Phases ◽  
Reversed Phase ◽  
Reversed Phase Hplc

Characterization of in vitro proteolytic processing of β-endorphin by reversed-phase HPLC

Peptides ◽  
1984 ◽  
Vol 5 (6) ◽  
pp. 1037-1042 ◽  
Author(s):  
Thomas P. Davis ◽  
Hans Schoemaker ◽  
Alison J. Culling-Berglund
Keyword(s):  
Reversed Phase ◽  
Proteolytic Processing ◽  
Reversed Phase Hplc

Chiral Chromatography Studies of Chemical Behavior of Cinacalcet on Polysaccharide Chiral Reversed-Phase HPLC Stationary Phases

2012 ◽  
Vol 95 (6) ◽  
pp. 1639-1643 ◽  
Author(s):  
Michal Douša ◽  
Jiřiacute; Břicháč
Keyword(s):  
Chiral Stationary Phase ◽  
Stationary Phases ◽  
Active Pharmaceutical Ingredient ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc ◽  
Chiral Chromatography ◽  
Chemical Behavior ◽  
Analytical Resolution

Abstract A rapid HPLC method for the analytical resolution of cinacalcet enantiomers was developed. Four chiral columns (two amylose and two cellulose type) were evaluated in RP systems. Excellent enantioseparation with a resolution of more than 6 was achieved on Chiralpak AY (amylose 5-chloro-2-methylphenylcarbamate chiral stationary phase) using 10 mM triethylamine (pH 8.0)–acetonitrile (40 + 60, v/v) mobile phase. Validation of the HPLC method, including linearity, LOD, LOQ, precision, accuracy, and selectivity, was performed according to the International Conference on Harmonization guidelines. The method was successfully applied for the determination of (S)-cinacalcet in enantiopure active pharmaceutical ingredient (R)-cinacalcet.

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