scholarly journals Dynamics of an Infectious Keratoconjunctivitis Outbreak by Mycoplasma conjunctivae on Pyrenean Chamois Rupicapra p. pyrenaica

PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e61887 ◽  
Author(s):  
MaríaCruz Arnal ◽  
Juan Herrero ◽  
Christian de la Fe ◽  
Miguel Revilla ◽  
Carlos Prada ◽  
...  
2003 ◽  
Vol 69 (4) ◽  
pp. 1913-1919 ◽  
Author(s):  
Luc Belloy ◽  
Martin Janovsky ◽  
Edy M. Vilei ◽  
Paola Pilo ◽  
Marco Giacometti ◽  
...  

ABSTRACT Mycoplasma conjunctivae is the etiological agent of infectious keratoconjunctivitis, a highly contagious ocular infection that affects both domestic and wild Caprinae species in the European Alps. In order to study the transmission and spread of M. conjunctivae across domestic and wild Caprinae populations, we developed a molecular method for subtyping and identifying strains of M. conjunctivae. This method is based on DNA sequence determination of a variable domain within the gene lppS, a gene that encodes an antigenic lipoprotein of M. conjunctivae. This domain of lppS shows variations among different strains but remains constant upon generations of individual strains on growth medium and thus allows identification of individual strains and estimation of their phylogenetic intercorrelations. The variable domain of lppS is amplified by PCR using primers that match conserved sequences of lppS flanking it. Sequence analysis of the amplified fragment enables fine subtyping of M. conjunctivae strains. The method is applicable both to isolated strains and to clinical samples directly without requiring the cultivation of the strain. Using this method, we show that M. conjunctivae was transmitted between domestic and wild animals that were grazing in proximate pastures. Certain animals also presented infections with two different strains simultaneously.


2001 ◽  
Vol 148 (8) ◽  
pp. 240-241 ◽  
Author(s):  
S. E. Baker ◽  
J. B. Bashiruddin ◽  
R. D. Ayling ◽  
R. A. J. Nicholas

2018 ◽  
Vol 44 (01) ◽  
pp. 33-39
Author(s):  
Wei-Hsun Chao ◽  
Jyh-Mirn Lai ◽  
Xiao-Xuan He ◽  
Yu-Hung Lin ◽  
Geng-Ruei Chang

Although infectious keratoconjunctivitis (IKC) has occurred worldwide, no report has mentioned an IKC case in a dairy goat farm in an indoor management system. Moreover, few clinicians follow the IKC syndrome development throughout the clinical course. The aim of the present study was to report an IKC outbreak caused by Mycoplasma conjunctivae (MC) in indoor dairy goat barns. Severe IKC breakouts had been found consecutively in two nearby goat barns in a cooperated farm. To confirm the etiology, samples were collected from the infected goats to perform hematological, biochemical, bacterial examinations and polymerase chain reactions. A treatment of 4% boric acid and 0.5% povidone iodine solution, and a preventive spray of quaternary ammonium at 09:00 AM and 1% Virkon-S[Formula: see text] solution at 17:00 PM were useful to control disease in the adult goat barn, wherein 60% (31/55) animals showed different IKC signs. A total of 12 (14%) goat kids, died, without treatment, showed variable degrees of bronchointerstitial pneumonia. In all cases, only the DNA fragments of MC, but not other pathogens implicated in IKC, were detected. Goats began to recover after 30 days in an episodic duration of 81 days resulting in an estimated 240,000 New Taiwan dollars loss. In conclusion, this is the first goat MC report in an indoor system. The treatment and the preventive measure described in the current study were useful to control the event.


2001 ◽  
Vol 32 (2) ◽  
pp. 155-164 ◽  
Author(s):  
Luc Belloy ◽  
Marco Giacometti ◽  
El-Mostafa Abdo ◽  
Jacques Nicolet ◽  
Margrit Krawinkler ◽  
...  

1999 ◽  
Vol 145 (20) ◽  
pp. 588-589 ◽  
Author(s):  
C. Grattarola ◽  
J. Frey ◽  
E.-M. Abdo ◽  
R. Orusa ◽  
J. Nicolet ◽  
...  

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