scholarly journals Artificial Receptors in Serologic Tests for the Early Diagnosis of Dengue Virus Infection

2006 ◽  
Vol 52 (8) ◽  
pp. 1486-1491 ◽  
Author(s):  
Dar-Fu Tai ◽  
Chung-Yin Lin ◽  
Tzong-Zeng Wu ◽  
Jyh-Hsiung Huang ◽  
Pei-Yun Shu
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Nonstructural Protein ◽  
Sample Pretreatment ◽  
Virus Infections ◽  
Serum Samples ◽  
Dengue Virus Infection ◽  
Artificial Receptors ◽  
Nonstructural Protein 1 ◽  
Sensitive Sensor

Abstract Background: Because of the range and nonspecificity of clinical presentations of dengue virus infections, we felt there was a need to create diagnostic tests. We used artificial receptors for the virus to develop serologic assays to detect dengue virus infection. Methods: We coated a quartz crystal microbalance (QCM) with molecularly imprinted polymers specific for nonstructural protein 1 of flavivirus. These artificial receptors were specifically created on a QCM chip by polymerization of monomers and were cross-linked in the presence of the epitope site of nonstructural protein 1. We tested serum samples from patients with confirmed cases of dengue reported to the Center for Disease Control in Taipei. Samples were diluted 100-fold; no other sample pretreatment was used. The QCM response was compared with results of monoclonal ELISA. Results: QCM signals were >15 Hz in 18 of 21 (86%) of dengue samples and in 0 of 16 control samples. The correlation (r2) of the QCM response and the ELISA result was 0.73. Within-run and run-to-run imprecisions (CV) were 4%–28% and 10%–32%, respectively. Conclusions: The described assay offers a serologic technique for diagnosis of early viremia. The results illustrate the potential of well-organized polymers on the highly sensitive sensor system for diagnostic and biotechnological applications.

scholarly journals Immunoglobulin A Antibody Responses in Dengue Patients: a Useful Marker for Serodiagnosis of Dengue Virus Infection

2005 ◽  
Vol 12 (10) ◽  
pp. 1235-1237 ◽  
Author(s):  
M. Nawa ◽  
T. Takasaki ◽  
M. Ito ◽  
S. Inoue ◽  
K. Morita ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Immunoglobulin A ◽  
Antibody Responses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Infections ◽  
Serum Samples ◽  
Dengue Virus Infection ◽  
Iga Antibody ◽  
Antibody Capture

ABSTRACT We determined the usefulness of an immunoglobulin A (IgA) antibody-capture enzyme-linked immunosorbent assay for serodiagnosis of dengue virus infections. The results indicate that the presence of IgA and IgM in serum samples assures recent primary dengue virus infection even with a single serum sample.

scholarly journals Prospective Study To Determine Accuracy of Rapid Serological Assays for Diagnosis of Acute Dengue Virus Infection in Laos

2007 ◽  
Vol 14 (11) ◽  
pp. 1458-1464 ◽  
Author(s):  
Stuart D. Blacksell ◽  
David Bell ◽  
James Kelley ◽  
Mammen P. Mammen ◽  
Robert V. Gibbons ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Patient Management ◽  
Limited Resource ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Infections ◽  
Serum Samples ◽  
Dengue Virus Infection ◽  
Resource Setting

ABSTRACT There is an urgent need for accurate and simple dengue virus infection diagnostic assays in limited-resource settings of dengue endemicity, to assist patient management. Using a panel of reference samples (S. D. Blacksell, P. N. Newton, D. Bell, J. Kelley, M. P. Mammen, D. W. Vaughn, V. Wuthiekanun, A. Sungkakum, A. Nisalak, and N. P. Day, Clin. Infect. Dis. 42:1127-1134, 2006), we recently evaluated eihgt commercially available immunochromatographic rapid diagnostic tests (RDTs) designed to detect dengue virus-specific immunoglobulin M (IgM) and/or IgG. We found that 6/8 RDTs had sensitivities of less than 50% (range, 6 to 65%), but specificities were generally high. Here, in conjuction with dengue virus serotyping by reverse transcriptase PCR and in the limited-resource setting of Laos, where dengue virus is endemic, we evaluated the same eight RDTs against a previously validated dengue IgM/IgG enzyme-linked immunosorbent assay for diagnosis of acute dengue virus infection. Paired serum samples were collected from 87 patients, of whom 38 had confirmed dengue virus infections (4 had primary infections, 33 had secondary infections, and 1 had an infection of indeterminate status). RDT sensitivity was low, with 7/8 RDTs having admission sample sensitivities of less than 20% (range, 4 to 26%). The majority (6/8) of the RDTs, demonstrated high specificity (>95%). Kappa statistic values ranged from 6 to 54% for the RDTs, demonstrating poor to moderate variation between three operators. No RDT adequately differentiated between primary and secondary dengue virus infections. The findings of this study suggest that currently available RDTs based on the detection of IgM antibodies for the diagnosis of acute dengue virus infections are unlikely to be useful for patient management.

scholarly journals Protection against Dengue Virus Infection in Mice by Administration of Antibodies against Modified Nonstructural Protein 1

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e92495 ◽  
Author(s):  
Shu-Wen Wan ◽  
Yi-Tien Lu ◽  
Chia-Hui Huang ◽  
Chiou-Feng Lin ◽  
Robert Anderson ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Nonstructural Protein ◽  
Dengue Virus Infection ◽  
Nonstructural Protein 1

Dengue outbreak 2018 in district Shangla KPK; clinical features and laboratory markers of dengue virus infection

2020 ◽  
Vol 15 (10) ◽  
pp. 693-699
Author(s):  
Faheem Anwar ◽  
Muhammad Tayyab ◽  
Muhammad Salman ◽  
Abdullah ◽  
Misbahud Din ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Nonstructural Protein ◽  
Immunoglobulin M ◽  
Dengue Virus Infection ◽  
Female Ratio ◽  
Dengue Outbreak ◽  
Nonstructural Protein 1 ◽  
Laboratory Markers ◽  
Dengue Outbreaks

Aim: To analyze and quantify the 2018 dengue outbreak which occurred in district Shangla, Pakistan. Materials & methods: 964 suspected dengue samples were collected and examined for clinical manifestation and laboratory markers. Results: In all, 375 suspected cases were confirmed with dengue virus infection using nonstructural protein 1 (NS1) antigen, immunoglobulin M (IgM) & Immunoglobulin G (IgG) antibodies and real-time PCR whereas PCR was 92.2% sensitive. The most prevalent serotype was dengue virus 3 (60.26%). The male/female ratio was 1.84 and the most highly affected tehsil was Alpuri. The most affected age group was 16–40 years (70.4%). A significant number of cases were reported in September (48.54%). Conclusion: Recurrence of the dengue outbreaks in the study area could alarmingly increase the mortality rate, therefore, proper measures are essential to control dengue epidemics in the future.

scholarly journals Re-evaluation of the pathogenic roles of nonstructural protein 1 and its antibodies during dengue virus infection

2013 ◽  
Vol 20 (1) ◽  
pp. 42 ◽  
Author(s):  
Yung-Chun Chuang ◽  
Shu-Ying Wang ◽  
Yee-Shin Lin ◽  
Hong-Ru Chen ◽  
Trai-Ming Yeh
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Nonstructural Protein ◽  
Dengue Virus Infection ◽  
Nonstructural Protein 1

scholarly journals Full Serotype- and Group-Specific NS1 Capture Enzyme-Linked Immunosorbent Assay for Rapid Differential Diagnosis of Dengue Virus Infection

2011 ◽  
Vol 18 (3) ◽  
pp. 430-434 ◽  
Author(s):  
Xixia Ding ◽  
Dongmei Hu ◽  
Yue Chen ◽  
Biao Di ◽  
Jing Jin ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Virus Infection ◽  
Dengue Virus ◽  
Nonstructural Protein ◽  
Dengue Infection ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dengue Virus Infection ◽  
Effective Prevention ◽  
Nonstructural Protein 1

ABSTRACTDengue virus (DENV), a member of theFlavivirusfamily, has four distinct serotypes (DENV serotype 1 [DENV1], DENV2, DENV3, and DENV4) that require differentiation for the effective prevention of morbid disease. Early and rapid differentiation between flaviviruses remains challenging. Full assays combining four individual, serotype-specific and one group-specific nonstructural protein 1 (NS1) antigen capture enzyme-linked immunosorbent assays (ELISAs) based on monoclonal antibodies (MAbs) against DENV NS1 were developed and validated. The sensitivities and specificities of the full NS1 ELISAs were evaluated with viral cultures and dengue acute-phase sera. Four serotype-specific NS1 ELISAs displayed high specificities for the detection and differentiation of appropriate serotypes. The group-specific NS1 ELISA was broadly reactive with the four dengue virus serotypes. None of the NS1 ELISAs displayed cross-reactivity with the other flaviviruses or samples from febrile patients with non-dengue virus infections. The full serotype- and group-specific MAb-based NS1 capture ELISAs may provide tools for the early detection and typing of dengue infection, which is preferable to reverse transcriptase PCR (RT-PCR) for the rapid differential diagnosis of dengue virus infection in the field.

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