The Bradford Method for Protein Quantitation

2003 ◽  
pp. 25-32 ◽  
Author(s):  
John B. W. Hammond ◽  
Nicholas J. Kruger
Keyword(s):  
Protein Quantitation ◽  
Bradford Method

Protein Quantitation Using Isotope-Assisted Mass Spectrometry

2010 ◽  
Vol 39 (1) ◽  
pp. 291-308 ◽  
Author(s):  
Kelli G. Kline ◽  
Michael R. Sussman
Keyword(s):  
Mass Spectrometry ◽  
Protein Quantitation

Automated Chloroform-Methanol Protein Extraction on the Biomek-FX Liquid Handler System v2

2022 ◽  
Author(s):  
Yan Chen ◽  
Tad Ogorzalek ◽  
Nurgul Kaplan Lease ◽  
Jennifer Gin ◽  
Christopher J Petzold
Keyword(s):  
Sample Preparation ◽  
Protein Extraction ◽  
Tryptic Digestion ◽  
Gram Negative ◽  
Protein Quantitation ◽  
System P ◽  
Automated Protocol ◽  
Chloroform Methanol ◽  
Extract Protein

This protocol details steps to extract protein from Gram-negative bacterial or fungal cells (that have been pretreated with zymolyase) in quantitative proteomic workflows by using a Biomek FX liquid handler system. It is a semi-automated protocol that includes several 'pause' steps for centrifugation steps that are conducted manually "off-deck". This protocol works best as part of an automated proteomic sample preparation workflow with: Automated Protein Quantitation with the Biomek-FX liquid handler system and Automated Protein Normalization and Tryptic Digestion on a Biomek-NX Liquid Handler System

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