The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds

2004 ◽  
pp. 65-74 ◽  
Author(s):  
William S. Pierpoint
Keyword(s):  
Phenolic Compounds ◽  
Plant Tissues

Tissue fixation and staining with osmium tetroxide: the role of phenolic compounds.

1978 ◽  
Vol 26 (2) ◽  
pp. 138-140 ◽  
Author(s):  
A J Nielson ◽  
W P Griffith
Keyword(s):  
Phenolic Compounds ◽  
Electron Density ◽  
Normal Tissue ◽  
Osmium Tetroxide ◽  
Direct Interaction ◽  
Plant Tissues ◽  
Tissue Fixation ◽  
Chelate Complexes ◽  
Animal Tissues

It has been postulated that phenol-containing areas of plant and animal tissues were osmiophilic, but proof of direct interaction between osmium tetroxide and phenolic materials, or the nature of such reactions, has been lacking. We find that, under conditions similar to those of normal tissue fixation, osmium tetroxide reacts rapidly with those phenols containing o-dihydroxy groups (including such species found in plant tissues) to give very stable chelate complexes. We conclude that these complexes are responsible for the observed electron-density in phenol-containing areas of tissue treated with osmium tetroxide, so that such phenols are indeed osmiophilic.

scholarly journals Silicon promotes the control of Meloidogyne incognita in lettuce by increasing ascorbic acid and phenolic compounds

2021 ◽  
Author(s):  
Tales Arthur de Souza Alonso ◽  
Dalila Lopes da Silva ◽  
Renato de Mello Prado ◽  
Pedro Luiz Martins Soares ◽  
Luis Felipe Lata Tenesaca ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Mechanisms Of Action ◽  
Plant Tissues ◽  
Barrier Effect ◽  
Physical Barrier ◽  
Chemical Action ◽  
Chemical Mechanisms ◽  
Second Stage ◽  
Chlorophyll Index

Abstract The use of silicon (Si) has a physical barrier effect on plant tissues, decreasing nematode infection in different crops. Notwithstanding, research on lettuce crop is lacking, especially regarding the chemical mechanisms of action of this beneficial element. Therefore, this study evaluates the effect of Si supply on lettuce plants infested with 0, 6000, and 12000 eggs and second stage juveniles of M. incognita, both in the absence and in the presence of Si (2 mM) in the nutrient solution. Silicon increases phenolic compounds and ascorbic acid, reducing the M. incognita population and decreasing oxidative stress. It also increases chlorophyll index and the quantum efficiency of the photosystem II (FV/FM), favoring the growth and production of lettuce plants. The use of Si decreased the number of nematodes and affected their reproduction, decreasing the number of eggs and galls in the roots of lettuce plants, being yet another sustainable alternative for the control of M. incognita. The Si benefit would be due to the combined effect of the physical barrier and the chemical action from the increase in phenolic compounds and ascorbic acid in plant tissues, improving the physiological aspects of plants.

Isolation of functional RNA from plant tissues rich in phenolic compounds

1991 ◽  
Vol 197 (1) ◽  
pp. 91-95 ◽  
Author(s):  
Alois Schneiderbauer ◽  
Heinrich Sandermann ◽  
Dieter Ernst
Keyword(s):  
Phenolic Compounds ◽  
Plant Tissues ◽  
Functional Rna

A simple method for extracting plant phenolic compounds

1992 ◽  
Vol 22 (5) ◽  
pp. 654-659 ◽  
Author(s):  
A. Sauvesty ◽  
F. Page ◽  
J. Huot
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Sugar Maple ◽  
Aqueous Ethanol ◽  
Quantitative Estimation ◽  
Plant Tissues ◽  
Simple Method ◽  
Extraction Solvent ◽  
Maple Leaves ◽  
Barley Leaves

Quantitative estimation of phenolic compounds in plant tissues remains uncertain, mainly because those substances are unstable and easily degradable. In this research we have developed and tested a new method for extracting phenolic compounds from sugar maple (Acersaccharum Marsh.) leaves. The research involved three steps: (i) various procedures currently used for extraction of phenolic compounds were tested with five pure phenolic acids; (ii) the extraction solvent, the procedure for dissolving the phenolic compounds, the temperature, and the duration of the treatment were tested on maple leaves; (iii) two methods that were found equally efficient for litter maple leaves were tested on maple leaves collected in June, on barley leaves, and on four pure phenolic acids. Based on those tests, the dissolution of phenolic compounds in 50% aqueous ethanol (v/v) at 40 °C for 3 h appeared to be the most reliable and the least destructive method. We also recommend the use of Polyclar AT, a resin that retains phenolics in solution, to assess the amount of reductive nonphenolic substances present in the plant material analyzed.

Demonstration of Phenolic Compounds in Plant Tissues by An Osmium-Iodide Postfixation Procedure

1989 ◽  
Vol 64 (6) ◽  
pp. 273-280 ◽  
Author(s):  
M. Scalet ◽  
E. Crivellato ◽  
F. Mallardi
Keyword(s):  
Phenolic Compounds ◽  
Plant Tissues
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