A simple and rapid method for RNA isolation from plant tissues with high phenolic compounds and polysaccharides

2007 ◽  
Author(s):  
Kam-Lock Chan ◽  
Chai-Ling Ho ◽  
Parameswari Namasivayam ◽  
Suhaimi Napis
Keyword(s):  
Phenolic Compounds ◽  
Rapid Method ◽  
Rna Isolation ◽  
Plant Tissues

scholarly journals Development of new total RNA isolation method for tissues with rich phenolic compounds

2020 ◽  
Vol 45 (4) ◽  
pp. 343-350
Author(s):  
Zafer Seçgin ◽  
Gökhan Gökdemir ◽  
Elif Seda Atabay ◽  
Aslıhan Kurt Kızıldoğan ◽  
Musa Kavas
Keyword(s):  
Phenolic Compounds ◽  
Rna Sequencing ◽  
Rna Isolation ◽  
Wall Structure ◽  
Isolation Method ◽  
High Quality ◽  
Ctab Method ◽  
Total Rna Isolation

AbstractBackgroundRNAs to be used in transcriptome analysis must be of high quality and pure in order to ensure maximum representation of the expressed genes. RNA isolation is difficult in hazelnut tissues containing large amounts of secondary metabolite, phenolic compounds and the cell wall structure. Commonly used protocols for RNA isolation are those that require a lot of labor and time and also do not allow sufficient RNA isolation when applied to tissues rich in phenolic compounds. This study was aimed to develop an efficient method for isolation of total RNAs from bud of hazelnut to be used in RNA sequencing.Materials and methodsAn optimized new method was successfully applied on three different hazelnuts genotypes (Çakıldak, Palaz, Tombul) and about 25 times higher amount of total RNAs per mg fresh tissues were obtained compared to classical CTAB method. Different methods have been tried for the isolation of RNA from hazelnut tissues and the determination of the quality of the obtained RNAs.ResultsThe quality and quantity of isolalated total RNAs were determined by spectrophotometer, electrophoresis and PCR. This success has been caught without any compromise of purity since A260/A280 ratios ranged from 1.90 to 2.04 and A260/A230 ratios were >2.0 in all purified RNAs.ConclusionThe total RNAs isolated with new protocol was found to be suitable for RNA sequencing and other molecular applications.

Tissue fixation and staining with osmium tetroxide: the role of phenolic compounds.

1978 ◽  
Vol 26 (2) ◽  
pp. 138-140 ◽  
Author(s):  
A J Nielson ◽  
W P Griffith
Keyword(s):  
Phenolic Compounds ◽  
Electron Density ◽  
Normal Tissue ◽  
Osmium Tetroxide ◽  
Direct Interaction ◽  
Plant Tissues ◽  
Tissue Fixation ◽  
Chelate Complexes ◽  
Animal Tissues

It has been postulated that phenol-containing areas of plant and animal tissues were osmiophilic, but proof of direct interaction between osmium tetroxide and phenolic materials, or the nature of such reactions, has been lacking. We find that, under conditions similar to those of normal tissue fixation, osmium tetroxide reacts rapidly with those phenols containing o-dihydroxy groups (including such species found in plant tissues) to give very stable chelate complexes. We conclude that these complexes are responsible for the observed electron-density in phenol-containing areas of tissue treated with osmium tetroxide, so that such phenols are indeed osmiophilic.

scholarly journals Silicon promotes the control of Meloidogyne incognita in lettuce by increasing ascorbic acid and phenolic compounds

2021 ◽  
Author(s):  
Tales Arthur de Souza Alonso ◽  
Dalila Lopes da Silva ◽  
Renato de Mello Prado ◽  
Pedro Luiz Martins Soares ◽  
Luis Felipe Lata Tenesaca ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Mechanisms Of Action ◽  
Plant Tissues ◽  
Barrier Effect ◽  
Physical Barrier ◽  
Chemical Action ◽  
Chemical Mechanisms ◽  
Second Stage ◽  
Chlorophyll Index

Abstract The use of silicon (Si) has a physical barrier effect on plant tissues, decreasing nematode infection in different crops. Notwithstanding, research on lettuce crop is lacking, especially regarding the chemical mechanisms of action of this beneficial element. Therefore, this study evaluates the effect of Si supply on lettuce plants infested with 0, 6000, and 12000 eggs and second stage juveniles of M. incognita, both in the absence and in the presence of Si (2 mM) in the nutrient solution. Silicon increases phenolic compounds and ascorbic acid, reducing the M. incognita population and decreasing oxidative stress. It also increases chlorophyll index and the quantum efficiency of the photosystem II (FV/FM), favoring the growth and production of lettuce plants. The use of Si decreased the number of nematodes and affected their reproduction, decreasing the number of eggs and galls in the roots of lettuce plants, being yet another sustainable alternative for the control of M. incognita. The Si benefit would be due to the combined effect of the physical barrier and the chemical action from the increase in phenolic compounds and ascorbic acid in plant tissues, improving the physiological aspects of plants.

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