The Use of Monoclonal Antibodies and Lectins to Identify Changes in Viral Glycoproteins That are Influenced by Glycosylation: The Case of Human Respiratory Syncytial Virus Attachment (G) Glycoprotein

ABSTRACT Human respiratory syncytial virus (RSV) F glycoprotein (RSV-F) can independently interact with immobilized heparin and facilitate both attachment to and infection of cells via an interaction with cellular heparan sulfate. RSV-glycosaminoglycan (GAG) interactions were evaluated using heparin-agarose affinity chromatography. RSV-F from A2- and B1/cp-52 (cp-52)-infected cell lysates, RSV-F derived from a recombinant vaccinia virus, and affinity-purified F protein all bound to and were specifically eluted from heparin columns. In infectivity inhibition studies, soluble GAGs decreased the infectivity of RSV A2 and cp-52, with bovine lung heparin exhibiting the highest specific activity against both A2 (50% effective dose [ED50] = 0.28 ± 0.11 μg/ml) andcp-52 (ED50 = 0.55 ± 0.14 μg/ml). Furthermore, enzymatic digestion of cell surface GAGs by heparin lyase I and heparin lyase III but not chondroitinase ABC resulted in a significant reduction in cp-52 infectivity. Moreover, bovine lung heparin inhibited radiolabeled A2 and cp-52 virus binding up to 90%. Taken together, these data suggest that RSV-F independently interacts with heparin/heparan sulfate and this type of interaction facilitates virus attachment and infectivity.

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Electron Microscopy of the Human Respiratory Syncytial Virus Fusion Protein and Complexes That It Forms with Monoclonal Antibodies

Virology ◽

10.1006/viro.2000.0279 ◽

2000 ◽

Vol 271 (1) ◽

pp. 122-131 ◽

Cited By ~ 77

Author(s):

Leslie J. Calder ◽

Luis González-Reyes ◽

Blanca García-Barreno ◽

Steve A. Wharton ◽

John J. Skehel ◽

...

Keyword(s):

Electron Microscopy ◽

Monoclonal Antibodies ◽

Respiratory Syncytial Virus ◽

Fusion Protein ◽

Human Respiratory Syncytial Virus ◽

Virus Fusion ◽

Syncytial Virus

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Preparation and Characterization of Monoclonal Antibodies Directed against Five Structural Components of Human Respiratory Syncytial Virus Subgroup B

Journal of General Virology ◽

10.1099/0022-1317-68-12-3125 ◽

1987 ◽

Vol 68 (12) ◽

pp. 3125-3135 ◽

Cited By ~ 61

Author(s):

C. Orvell ◽

E. Norrby ◽

M. A. Mufson

Keyword(s):

Monoclonal Antibodies ◽

Respiratory Syncytial Virus ◽

Human Respiratory Syncytial Virus ◽

Structural Components ◽

Syncytial Virus

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Bovine respiratory syncytial virus: immunohistochemichal detection in mouse and bovine tissues using a Mab against human respiratory syncytial virus

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/s0102-09352006000600001 ◽

2006 ◽

Vol 58 (6) ◽

pp. 973-981 ◽

Cited By ~ 1

Author(s):

R.S. Almeida ◽

F.R. Spilki ◽

P.M. Roehe ◽

L.M.C. Verinaud ◽

C.W. Arns

Keyword(s):

Monoclonal Antibody ◽

Monoclonal Antibodies ◽

Respiratory Syncytial Virus ◽

Respiratory Disease ◽

Cell Cultures ◽

Post Infection ◽

Bovine Respiratory Syncytial Virus ◽

Human Respiratory Syncytial Virus ◽

Clinical Samples ◽

Syncytial Virus

An immunoistochemical (IHC) test was developed to detect bovine respiratory syncytial virus (BRSV) in cell cultures and tissues of experimentally infected mice and calves, using a commercial monoclonal antibody (Mab) against human respiratory syncytial virus (HRSV), as a less expensive alternative, instead of producing specific monoclonal antibodies to BRSV. Clinical samples from calves suffering respiratory disease were also submitted to this test. IHC detected BRSV antigens in mouse tracheas (3, 5 and 7 days post-infection) and lungs (5 and 7 days post-infection), and in one of three lungs from experimentally infected calves. Lungs samples from two naturally infected calves were tested and resulted positive for BRSV by the IHC test. These results suggest that this test may be used in the future for diagnosis as well as a useful tool to assess the distribution of BRSV infections in Brazilian herds.

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