scholarly journals Detection and characterization of avian hepatitis E virus from broiler breeders and layers in Korea

2018 ◽  
Vol 58 (1) ◽  
pp. 45-49
Author(s):  
Hyun-Woo Moon ◽  
Haan Woo Sung ◽  
Hyuk Moo Kwon
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Broiler Breeders ◽  
Avian Hepatitis E Virus

Identification and characterization of avian hepatitis E virus genotype 2 from chickens with hepatitis-splenomegaly syndrome in Korea

Virus Genes ◽  
2016 ◽  
Vol 52 (5) ◽  
pp. 738-742 ◽  
Author(s):  
Hyun-Woo Moon ◽  
Byung-Woo Lee ◽  
Haan Woo Sung ◽  
Byung-Il Yoon ◽  
Hyuk Moo Kwon
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Genotype ◽  
Genotype 2 ◽  
Avian Hepatitis E Virus ◽  
Identification And Characterization

scholarly journals Distribution and molecular characterization of avian hepatitis E virus (aHEV) in domestic and wild birds in Burkina Faso

2019 ◽  
Vol 11 (2) ◽  
pp. 45-50
Author(s):  
B. Ouoba Jean ◽  
A. Traore Kuan ◽  
K. M’Bengue Alphonsine ◽  
Ngazoa Solange ◽  
Rouamba Hortense ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Molecular Characterization ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Wild Birds ◽  
Avian Hepatitis E Virus

Characterization of antigenic domains and epitopes in the ORF3 protein of a Chinese isolate of avian hepatitis E virus

2013 ◽  
Vol 167 (3-4) ◽  
pp. 242-249 ◽  
Author(s):  
Qin Zhao ◽  
Ya-ni Sun ◽  
Shou-bin Hu ◽  
Xin-jie Wang ◽  
Yi-hong Xiao ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Chinese Isolate ◽  
Orf3 Protein ◽  
Antigenic Domains ◽  
Avian Hepatitis E Virus

Infection of Pigs with Avian Hepatitis E Virus (HEV)

2005 ◽  
Author(s):  
C. Kasorndorkbua ◽  
P. J. Thomas ◽  
Patrick G. Halbur ◽  
D. K. Guenette ◽  
F. F. Huang ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Avian Hepatitis E Virus

scholarly journals Antigenic Characterization of ORF2 and ORF3 Proteins of Hepatitis E Virus (HEV)

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1385
Author(s):  
Giulia Pezzoni ◽  
Lidia Stercoli ◽  
Eleonora Pegoiani ◽  
Emiliana Brocchi
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Recombinant Antigen ◽  
Open Reading Frame ◽  
Reading Frame ◽  
E Coli ◽  
Antigenic Characterization ◽  
Antigenic Properties ◽  
Open Reading Frame 2

To evaluate the antigenic properties of Hepatitis E Virus (HEV) Open Reading Frame 2 and 3 (ORF2 and ORF3) codified proteins, we expressed different portions of ORF2 and the entire ORF3 in E. coli, a truncated ORF2, was also expressed in baculovirus. A panel of 37 monoclonal antibodies (MAbs) was raised against ORF2 (1–660 amino acids) and MAbs were mapped and characterized using the ORF2 expressed portions. Selected HEV positive and negative swine sera were used to evaluate ORF2 and ORF3 antigens’ immunogenicity. The MAbs were clustered in six groups identifying six antigenic regions along the ORF2. Only MAbs binding to the sixth ORF2 antigenic region (394–608 aa) were found to compete with HEV positive sera and efficiently catch the recombinant antigen expressed in baculovirus. The ORF2 portion from 394–608 aa demonstrated to include most immunogenic epitopes with 85% of HEV positive swine sera reacting against the region from 461–544 aa. Only 5% of the selected HEV sera reacted against the ORF3 antigen.

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