Effect of nitrogen and phosphorus on the development and differentiation of vegetative cells of Porphyra yezoensis on solid agar medium

2006 ◽  
Vol 49 (5/6) ◽  
Author(s):  
Songdong Shen ◽  
Lihong He ◽  
Pu Xu ◽  
Zhu Jianyi
Keyword(s):  
Agar Medium ◽  
Porphyra Yezoensis ◽  
Nitrogen And Phosphorus ◽  
Vegetative Cells ◽  
Solid Agar ◽  
Development And Differentiation

scholarly journals Impact of Temperature, Osmotic Potential, and Osmoregulant on the Growth of Three Ectotrophic Root-Infecting Fungi of Kentucky Bluegrass

Plant Disease ◽  
1997 ◽  
Vol 81 (8) ◽  
pp. 873-879 ◽  
Author(s):  
Karen A. Plumley ◽  
Ann B. Gould ◽  
Bruce B. Clarke
Keyword(s):  
Osmotic Potential ◽  
Agar Medium ◽  
Fungal Growth ◽  
Dry Weight ◽  
Kentucky Bluegrass ◽  
Magnaporthe Poae ◽  
Daily Rate ◽  
Solid Agar ◽  
Leptosphaeria Korrae ◽  
Osmotic Potentials

Two isolates each of Magnaporthe poae, Gaeumannomyces incrustans, and Leptosphaeria korrae were grown at 25°C in liquid shake culture in minimal salts medium (-0.12 MPa) or minimal salts medium adjusted to -0.5 MPa with KCl, MgCl2, or polyethylene glycol (PEG). Fungal dry weight of all three species was greater in minimal salts medium amended to -0.5 MPa with MgCl2 than in nonamended medium, and dry weight in medium amended with PEG was not different from dry weights in nonamended medium or medium amended with KCl. Fungi were incubated at varying temperatures on a minimal salts solid-agar medium (-0.12 MPa) adjusted to osmotic potentials ranging from -0.5 to -5.0 MPa with KCl or MgCl2. Optimum growth of M. poae, G. incrustans, and L. korrae on nonamended medium occurred at 30, 30, and 25°C, respectively. At optimum temperatures for each species, fungal growth was greatest at the higher osmotic potentials tested (-0.5 to -1.0 MPa) and decreased in a linear manner as osmotic potential decreased. In most cases, growth was detected at the lowest osmotic potential measured (-5.0 MPa). The relationship of fungal growth to osmotic potential depended on both temperature and osmoregulant. At temperatures optimal or nearly optimal for fungal development, the growth of all three fungi declined more rapidly with decreasing osmotic potential when grown on medium amended with MgCl2 than on medium amended with KCl. At the highest temperature evaluated for growth of M. poae and L. korrae (35 and 30°C, respectively), growth on medium amended with KCl was curvilinear and peaked at osmotic potentials of -2.5 to -3.0 MPa. Furthermore, between osmotic potentials of -2.0 and -5.0 MPa, M. poae grew best at 35°C. When maintained on nonamended minimal salts medium (-0.12 MPa) in liquid culture at 25°C or on nonamended solid-agar medium at temperatures optimal for growth, M. poae grew at a faster daily rate than L. korrae.

scholarly journals WdStuAp, an APSES Transcription Factor, Is a Regulator of Yeast-Hyphal Transitions in Wangiella (Exophiala) dermatitidis

2007 ◽  
Vol 6 (9) ◽  
pp. 1595-1605 ◽  
Author(s):  
Qin Wang ◽  
Paul J. Szaniszlo
Keyword(s):  
Transcription Factor ◽  
Agar Medium ◽  
Hyphal Growth ◽  
Surface Growth ◽  
Yeast Growth ◽  
Amino Terminal ◽  
Yeast Extract Peptone Dextrose ◽  
Development And Differentiation ◽  
Colony Surface ◽  
The Rich

ABSTRACT APSES transcription factors are well-known regulators of fungal cellular development and differentiation. To study the function of an APSES protein in the fungus Wangiella dermatitidis, a conidiogenous and polymorphic agent of human phaeohyphomycosis with yeast predominance, the APSES transcription factor gene WdSTUA was cloned, sequenced, disrupted, and overexpressed. Analysis showed that its derived protein was most similar to the APSES proteins of other conidiogenous molds and had its APSES DNA-binding domain located in the amino-terminal half. Deletion of WdSTUA in W. dermatitidis induced convoluted instead of normal smooth colony surface growth on the rich yeast maintenance agar medium yeast extract-peptone-dextrose agar (YPDA) at 37°C. Additionally, deletion of WdSTUA repressed aerial hyphal growth, conidiation, and invasive hyphal growth on the nitrogen-poor, hypha-inducing agar medium potato dextrose agar (PDA) at 25°C. Ectopic overexpression of WdSTUA repressed the convoluted colony surface growth on YPDA at 37°C, and also strongly repressed hyphal growth on PDA at 25°C and 37°C. These new results provide additional insights into the diverse roles played by APSES factors in fungi. They also suggest that the transcription factor encoded by WdSTUA is both a positive and negative morphotype regulator in W. dermatitidis and possibly other of the numerous human pathogenic, conidiogenous fungi capable of yeast growth.

scholarly journals Semi-Solid Agar Medium for Detection of Fungal Enzymes

2019 ◽  
Vol 47 (2) ◽  
pp. 99-119
Author(s):  
Naglaa Muhanna
Keyword(s):  
Agar Medium ◽  
Fungal Enzymes ◽  
Solid Agar ◽  
Semi Solid

Effect of nitrogen and phosphorus on the growth and amino acid contents of Porphyra yezoensis

2016 ◽  
Vol 48 (6) ◽  
pp. 2798-2802 ◽  
Author(s):  
Xinshu Li ◽  
Peiming He ◽  
Juntian Xu ◽  
Guanghui Fu ◽  
Yaobai Chen
Keyword(s):  
Amino Acid ◽  
Porphyra Yezoensis ◽  
Nitrogen And Phosphorus ◽  
Amino Acid Contents

scholarly journals Mycobacterium and Aerobic Actinomycete Culture: Are Two Medium Types and Extended Incubation Times Necessary?

2016 ◽  
Vol 54 (4) ◽  
pp. 1089-1093 ◽  
Author(s):  
Patricia J. Simner ◽  
Kelly A. Doerr ◽  
Lory K. Steinmetz ◽  
Nancy L. Wengenack
Keyword(s):  
Liquid Medium ◽  
Chart Review ◽  
Solid Medium ◽  
Nontuberculous Mycobacteria ◽  
Agar Medium ◽  
Content Type ◽  
Medical Chart Review ◽  
Solid Agar ◽  
Incubation Periods ◽  
Aerobic Actinomycetes

Mycobacterial cultures are historically performed using a liquid medium and a solid agar medium with an incubation period of up to 60 days. We performed a retrospective analysis of 21,494 mycobacterial and aerobic actinomycetes cultures performed over 10 months to determine whether two medium types remain necessary and to investigate whether culture incubation length can be shortened. Specimens were cultured using Bactec MGIT liquid medium and Middlebrook 7H11/S7H11 solid medium with incubation periods of 42 and 60 days, respectively. Time-to-positivity and the identity of isolates recovered from each medium were evaluated. A total of 1,205/21,494 cultures (6%) were positive on at least one medium. Of the 1,353 isolates recovered, 1,110 (82%) were nontuberculous mycobacteria, 145 (11%) were aerobic actinomycetes, and 98 (7%) wereMycobacterium tuberculosiscomplex. Assessing medium types, 1,121 isolates were recovered from solid medium cultures, 922 isolates were recovered from liquid medium cultures, and 690 isolates were recovered on both media. Liquid cultures were positive an average of 10 days before solid cultures when the two medium types were positive (P< 0.0001). Isolates detected on solid medium after 6 weeks of incubation included 65 (5%) nontuberculous mycobacteria, 4 (0.3%) aerobic actinomycetes, and 2 (0.2%) isolates from theM. tuberculosiscomplex. Medical chart review suggested that most of these later-growing isolates were insignificant, as the diagnosis was already known, or they were considered colonizers/contaminants. This study reaffirms the need for both liquid medium and solid medium for mycobacterial and aerobic actinomycetes culture and demonstrates that solid medium incubation times may be reduced to 6 weeks without significantly impacting sensitivity.

Morphological and photosynthetic variations in the process of spermatia formation from vegetative cells in Porphyra yezoensis Ueda (Bangiales, Rhodophyta) and their responses to desiccation

Planta ◽  
2011 ◽  
Vol 235 (5) ◽  
pp. 885-893 ◽  
Author(s):  
Rui-Ling Yang ◽  
Wei Zhou ◽  
Song-Dong Shen ◽  
Guang-Ce Wang ◽  
Lin-Wen He ◽  
...  
Keyword(s):  
Porphyra Yezoensis ◽  
Vegetative Cells

scholarly journals Comparison of Agar and an Agitated, Thin-film, Liquid System for Micropropagation of Ornamental Elephant Ears

HortScience ◽  
2004 ◽  
Vol 39 (5) ◽  
pp. 1088-1092 ◽  
Author(s):  
Jeffrey Adelberg ◽  
Joe Toler
Keyword(s):  
Thin Film ◽  
Growth Medium ◽  
Agar Medium ◽  
Dry Weight ◽  
Liquid System ◽  
Multiplication Rate ◽  
Film Culture ◽  
Agar Culture ◽  
Solid Agar ◽  
Semi Solid

Micropropagation of black-stemmed elephant ear (C. esculenta (L.) Schott `Fontanesii')' and upright elephant ear (A. macrorrhizos G. Don) were compared in semi-solid agar media and agitated, liquid thin-film bioreactor vessels at four explant densities (33, 100, 165, and 330 explants/L of media) using two growth regulator combinations: 1) 1 μm benzylaminopurine (BA)—growth medium, and 2) 3 μm BA plus 3 μm ancymidol—multiplication medium. The thin-film liquid system outperformed agar culture for most measured responses. Some exceptions were relative dry weights at higher explant densities and multiplication rate of Colocasia. When the thin-film liquid system was compared to agar culture, Alocasia explants produced their greatest biomass and had the least residual sugar at the highest explant density. Alocasia explants multiplied most rapidly and had the greatest relative dry weight on liquid media at the low explant densities. Alocasia plants were larger in growth medium than multiplication medium and larger in liquid medium than agar medium. When compared to agar, Colocasia in the thin-film liquid system produced the greatest biomass at the highest explant density in growth medium, had the greatest relative dry weight at the lowest explant density, and used the most sugar at the highest explant density. Alocasia and Colocasia would likely produce greater fresh and dry weight at the highest explant density if additional sugar were supplied during thin-film culture. Greater growth in thin-film culture of Alocasia and Colocasia is due in part, to greater availability of sugar in liquid compared to agar medium.

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