A Comparative Study on the Genotoxic Effect of Pyrimethamine in Bone Marrow and Spermatogonial Mice Cells

2007 ◽  
Vol 62 (9-10) ◽  
pp. 679-683 ◽  
Author(s):  
Serap Çelikler ◽  
Nilüfer Aydemir ◽  
Rahmi Bilaloğlu
Keyword(s):  
Bone Marrow ◽  
Comparative Study ◽  
Chromosomal Aberrations ◽  
Acute Treatment ◽  
Bone Marrow Cells ◽  
Single Treatment ◽  
Mutagenic Potential ◽  
Antimalarial Agent ◽  
Dose Levels ◽  
Marrow Cells

Pyrimethamine is an antimalarial agent widely used in clinical therapy. We aimed to compare its mutagenic potential in mammalian spermatogonial and bone marrow cells. For studying chromosomal aberrations mice were treated acutely (single treatment) with 4 dose levels of pyrimethamine (5, 10, 20 and 40 mg/kg). Pyrimethamine was found to produce a significant increase in structural chromosomal aberrations after acute treatment in bone marrow cells of mice (p < 0.001). It also induced chromosome abnormalities in spermatogonial cells (p < 0.05) at the highest dose.

scholarly journals Brahmarasayana protects against Ethyl methanesulfonate or Methyl methanesulfonate induced chromosomal aberrations in mouse bone marrow cells

2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Kanive Parashiva Guruprasad ◽  
Advait Subramanian ◽  
Vikram Jeet Singh ◽  
Raghavendra Sudheer Kumar Sharma ◽  
Puthiya Mundyat Gopinath ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Chromosomal Aberrations ◽  
Bone Marrow Cells ◽  
Ethyl Methanesulfonate ◽  
Methyl Methanesulfonate ◽  
Mouse Bone Marrow ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells

Induction of chromosomal aberrations in bone marrow cells of asbestotic rats

1992 ◽  
Vol 57 (2) ◽  
pp. 175-180 ◽  
Author(s):  
Nahid Fatma ◽  
Sikandar G. Khan ◽  
Mohammad Aslam ◽  
Qamar Rahman
Keyword(s):  
Bone Marrow ◽  
Chromosomal Aberrations ◽  
Bone Marrow Cells ◽  
Marrow Cells

Beta-carotene as a modulator of chromosomal aberrations induced in mouse bone marrow cells

1992 ◽  
Vol 20 (3) ◽  
pp. 206-210 ◽  
Author(s):  
Daisy M. F. Salvadori ◽  
Lúcia R. Ribeiro ◽  
Marília D. M. Oliveira ◽  
Carlos A. B. Pereira ◽  
Willy Beçak
Keyword(s):  
Bone Marrow ◽  
Chromosomal Aberrations ◽  
Bone Marrow Cells ◽  
Beta Carotene ◽  
Mouse Bone Marrow ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells

scholarly journals Genotoxic action of the sesquiterpene lactone glaucolide B on mammalian cells in vitro and in vivo

1999 ◽  
Vol 22 (3) ◽  
pp. 401-406 ◽  
Author(s):  
Regislaine V. Burim ◽  
Renata Canalle ◽  
João L. Callegari Lopes ◽  
Catarina S. Takahashi
Keyword(s):  
Bone Marrow ◽  
Sesquiterpene Lactone ◽  
Chromosomal Aberrations ◽  
Mammalian Cells ◽  
Culture Medium ◽  
Bone Marrow Cells ◽  
Proliferation Index ◽  
Marrow Cells

Glaucolide B is a sesquiterpene lactone isolated from Vernonia eremophila Mart. (Vernonieae, Asteraceae) and has schistosomicidal, antimicrobial and analgesic activities. This study examined the cytotoxic and clastogenic activities of glaucolide B in human cultured lymphocytes and in bone marrow cells from BALB/c mice. The mitotic index (MI) and chromosomal aberrations were analyzed in both of the above systems, whereas sister chromatid exchanges (SCE) and the proliferation index (PI) were determined only in vitro. In human cultured lymphocytes, glaucolide B concentrations greater than 15 µg/ml of culture medium completely inhibited cell growth. At 4 µg/ml and 8 µg/ml of culture medium, glaucolide B significantly increased the frequency of chromosomal aberrations in lymphocytes and was also cytotoxic at concentrations ³8 µg/ml; there was no increase in the frequency of SCE. Glaucolide B (160-640 mg/kg) did not significantly increase the frequency of chromosomal aberrations in mouse bone marrow cells nor did it affect cell division. Since glaucolide B showed no clastogenic action on mammalian cells in vivo but was cytotoxic and clastogenic in vitro, caution is needed in its medicinal use.

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